Involvement of regional lymph nodes after penetration of Schistosoma mansoni cercariae in naive and infected mice

Lozzi, Silene P.; Machado, Conceição R. S.; Gerken, S. E.; Mota-Santos, T. A.

doi:10.1590/s0074-02761996000400017

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…Despite this, relatively few studies can assign a clear in vivo function to eosinophils. In vitro, destruction of helminth larvae has been observed in several settings and eosinophils were shown to participate in an antibody-dependent manner (6,8,25). The direct involvement of various eosinophil-derived granule proteins against microfilariae in vitro was observed at a relatively high concentration (21).…”

Section: Discussionmentioning

confidence: 99%

“…Data supporting eosinophil function in vivo is mainly indirect since the evidence refers to interference in interleukin-5 (IL-5)-dependent responses, which are known for recruitment of eosinophils to the site of infection (1,25,29) Thus, in nonpermissive models of filarial infection, blockade of IL-5-dependent pathways resulted in a higher number of transient worm stages (23,26). In the permissive model of murine infection with Litomosoides sigmodontis, our group previously showed that IL-5 is a key cytokine for protection against invading L3 larvae, nodule formation, and control of microfilariae (2,43).…”

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confidence: 99%

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Lack of Eosinophil Peroxidase or Major Basic Protein Impairs Defense against Murine Filarial Infection

Specht¹,

Saeftel²,

Arndt

et al. 2006

Infect Immun

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Eosinophils are a hallmark of allergic diseases and helminth infection, yet direct evidence for killing of helminth parasites by their toxic granule products exists only in vitro. We investigated the in vivo roles of the eosinophil granule proteins eosinophil peroxidase (EPO) and major basic protein 1 (MBP) during infection with the rodent filaria Litomosoides sigmodontis. Mice deficient for either EPO or MBP on the 129/SvJ background developed significantly higher worm burdens than wild-type mice. Furthermore, the data indicate that EPO or MBP is involved in modulating the immune response leading to altered cytokine production during infection. Thus, in the absence of MBP, mice showed increased interleukin-10 (IL-10) production after stimulation of macrophages from the thoracic cavity where the worms reside. In addition to elevated IL-10 levels, EPO ؊/؊ mice displayed strongly increased amounts of the Th2 cytokine IL-5 by CD4 T cells as well as a significantly higher eosinophilia. Interestingly, a reduced ability to produce IL-4 in the knockout strains could even be seen in noninfected mice, arguing for different innate propensities to react with a Th2 response in the absence of either EPO or MBP. In conclusion, both of the eosinophil granule products MBP and EPO are part of the defense mechanism against filarial parasites. These data suggest a hitherto unknown interaction between eosinophil granule proteins, defense against filarial nematodes, and cytokine responses of macrophages and CD4 T cells.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Lack of Eosinophil Peroxidase or Major Basic Protein Impairs Defense against Murine Filarial Infection

Specht¹,

Saeftel²,

Arndt

et al. 2006

Infect Immun

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“…The opossum lymphatic-sinus mast cells (LSMC) differed from the opossum CTMC by their larger size and enlarged cytoplasmic granules that were also more heterogeneous in shape and staining properties (ChiariniGarcia and Machado 1992). The presence of a unique population of mast cells in the opposum lymph nodes is an interesting finding since no dissimilarity with CTMC has been reported for mast cells of eutherian lymph nodes (Sainte-Marie and Peng 1990; Lozzi et al 1996). The abundance of LSMC in opossum lymph nodes contrasts with the usually smaller proportion of mast cells in lymph nodes of eutherian mammals.…”

Section: Introductionmentioning

confidence: 92%

Mast cell types and cell-to-cell interactions in lymph nodes of the opossum Didelphis albiventris

Chiarini-Garcia¹,

Santos

Machado³

2000

Anatomy and Embryology

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Previous light-microscopic studies have shown a unique population of mast cells in lymphatic sinuses of lymph nodes located in the head, neck, axillary fossa and inguinal region of the opossum. In the present work, scanning and transmission electron-microscopic studies in the opossum mandibular and superficial axillary lymph nodes have strengthened the differences between connective-tissue mast cells (CTMC) and the lymphatic-sinus mast cells (LSMC). Further, close appositions of mast cells to other cells were described. At the nodal capsule, CTMC contacted fibroblast and granulocytes. In the lymphatic sinuses a few CTMC contacted LSMC, macrophages and reticular cells. The LSMC contacted macrophages, reticular cells and other LSMC. A few LSMC could be located in the medullary cord in close contact with plasma cells or other lymphoid cells, keeping the same ultrastructural features of those found in the lymphatic sinuses. An important new finding was provided by light-microscopic studies in nine abdominal lymph nodes. Most of them (para-aortic, common iliac, cardial, cecocolic and those of the body and tail of the pancreas) displayed numerous LSMC with the same distribution and histological features described herein. However, the mesenteric, pyloric and head-of-pancreas lymph nodes were virtually devoid of LSMC. Instead, their mast cells occurred mainly at the medullary cords and were very similar to the CTMC. Ultrastructural studies at the mesenteric lymph nodes confirmed the CTMC character of the mast cells located at both medullary cords and sinuses, and disclosed interactions with macrophages and lymphoid cells.

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“…According to Bloch (1984), the reason for the discrepancy may be that in vivo the parasites are metabolically more active and alter their surface membranes more rapidly, so that cell adherence is not so effective as in in vitro environment. Lozzi et al (1996) showed that S. mansoni primary and re-infection induced an influx of eosinophils to regional lymph nodes. However, only in the reinfected animals, were the eosinophils able to adhere to the larval surface, damaged larval being found inside eosinophilic infiltrates.…”

Section: Discussionmentioning

confidence: 99%

Immunological system and Schistosoma mansoni: co-evolutionary immunobiology. What is the eosinophil role in parasite-host relationship?

Lenzi¹,

Pacheco²,

Pelajo-Machado³

et al. 1997

Mem. Inst. Oswaldo Cruz

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Involvement of regional lymph nodes after penetration of Schistosoma mansoni cercariae in naive and infected mice

Abstract: The parotid lymph nodes of naive and previously infected Balb/c mice were studied after, respectively, infection and re-infection

Cited by 7 publications

References 25 publications

Lack of Eosinophil Peroxidase or Major Basic Protein Impairs Defense against Murine Filarial Infection

Lack of Eosinophil Peroxidase or Major Basic Protein Impairs Defense against Murine Filarial Infection

Mast cell types and cell-to-cell interactions in lymph nodes of the opossum Didelphis albiventris

Immunological system and Schistosoma mansoni: co-evolutionary immunobiology. What is the eosinophil role in parasite-host relationship?

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