Eosinophilia

We, Parish

doi:10.1111/j.1365-2222.1972.tb01302.x

Cited by 30 publications

(8 citation statements)

References 14 publications

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“…When a chemotactic factor which preferentially attracted eosinophils was shown to be released from human mast cells (8) and basophils (27,28), the activity was found to reside in peptides that could be distinguished from histamine by size, charge, and susceptibility to enzymes (6) . This factor was designated eosinophil chemotactic factor of anaphylaxis (ECF,) .…”

Section: Discussionmentioning

confidence: 99%

The selective eosinophil chemotactic activity of histamine.

Clark¹,

Gallin²,

Kaplan³

1975

The Journal of Experimental Medicine

246

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Histamine diphosphate was shown to selectively attract human eosinophils from mixed granulocyte populations when over 20% eosinophils were used in a modified Boyden chamber chemotactic assay system. This effect of histamine is abolished by incubation with diamine oxidase (histaminase) and was generated by decarboxylation of L-histidine. A linear dose dependent increase in eosinophil migration was observed between 3 X 10(-7) M and 1.25 X 10(-6) M, while higher concentrations of histamine inhibited the migration of eosinophils. The attractant activity of histamine was not inhibited by H-1 or H-2 receptor antagonists, however, the inhibition of migration observed at higher histamine concentrations was reversed by metiamine, an H-2 receptor antagonist. The effects of histamine upon eosinophil migration were demonstrable using three different assays: (a) counting cells that had traversed 5-mum pore, 12-mum thick polycarbonate filters, (b) counting cells that had migrated various distances into a 3-mum pore, 145-mum cellulose nitrate filters, or (c) measuring the number of cells that had traversed an upper polycarbonate filter and migrated into a lower cellulose nitrate filter using 15Cr-labeled cells. The ability of histamine to enhance eosinophil migration was shown to be dependent upon the presence of a concentration gradient; histamine did not cause a dose-dependent increase in random motility. Furthermore, preincubation of the eosinophils with histamine deactivate the cells to further stimulation by histamine or by C5a. It is concluded that in low doses histamine is a chemoattractant for human eosinophils, while in higher doses histamine inhibits eosinophil migration. These observations may relate to the influx and localization of eosinophils in immediate hypersensitivity reactions.

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Section: Discussionmentioning

confidence: 99%

The selective eosinophil chemotactic activity of histamine.

Clark¹,

Gallin²,

Kaplan³

1975

The Journal of Experimental Medicine

246

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“…Low molecular weight (300-500 daltons) eosinophil chemotactic factor (ECF) was first described in mast cells [1] and ba sophils [2], and since then, a similar ECF has been reported in human neutrophils and eosinophils (3] and more recently in murine macrophages [4).…”

Section: Introductionmentioning

confidence: 99%

Evidence for the Non-Peptide Nature of Neutrophil-Derived Eosinophil Chemotactic Factor

Czarnetzki¹,

Zimmermann²

1981

Int Arch Allergy Immunol

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Neutrophil-derived low molecular weight eosinophil chemotactic factor (ECF_PMN) was examined by a number of methods to prove its peptide nature. Sephadex G-10 eluates of ECF_PMN were analyzed by paper chromatography and thin-layer chromatography, and peptides were separated on a peptide analyzer. Peptides isolated by these three different methods have no neutrophil or eosinophil chemotactic activity over a wide range of concentrations. Enzyme digestion studies supported these findings. Other physical and biological properties of ECF_PMN suggest that this material is most likely lipid in nature.

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“…This study also indicates a similar fiuctuation in the presence of eosinophilic granulocytes in the sputum. Parish (1972) has suggested that basophils may elaborate a substance that is chemotactic for eosinophils. The elaboration of such a chemotactic substance may explain the observed parallel fiuctuation in basophil and eosinophil numbers in the sputa of bronchial asthmatics.…”

Section: Discussionmentioning

confidence: 99%

Appearance of basophils in the sputum of patients with bronchial asthma

Kimura

Tanizaki

Saito

et al. 1975

Clin Experimental Allergy

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Summary A total of 108 samples of sputum obtained from twenty patients with bronchial asthma were examined for appearance of basophils and eosinophils. Both cell types are present in sputum during an asthmatic attack and disappear at the conclusion of the attack. Their presence correlates with the severity of the disease. It has previously been demonstrated that the blood basophils count falls during attacks of bronchial asthma, and the present study suggests that basophils move from the blood stream into bronchial tissue during the acute phase of an asthmatic attack.

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Eosinophilia

Cited by 30 publications

References 14 publications

The selective eosinophil chemotactic activity of histamine.

The selective eosinophil chemotactic activity of histamine.

Evidence for the Non-Peptide Nature of Neutrophil-Derived Eosinophil Chemotactic Factor

Appearance of basophils in the sputum of patients with bronchial asthma

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