Eosinophil protein in airway macrophages: A novel biomarker of eosinophilic inflammation in patients with asthma

Kulkarni, Neeta; Hollins, Fay; Sutcliffe, Amanda; Saunders, Ruth; Sj, Shah; Siddiqui, Salman; Gupta, Sumit; Haldar, Pranabashis; Green, Ruth; Pavord, Ian D.; Wardlaw, Andrew J.; Brightling, Christopher E.

doi:10.1016/j.jaci.2010.03.026

Cited by 73 publications

(79 citation statements)

References 26 publications

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“…Sputum mediator measurements were performed using the sensitive Meso Scale Discovery Platform (Meso Scale Discovery, Gaithersburg, MD) for eight mediators that we had previously established to be valid after spiking experiments (28). In half of the bronchoscopy subjects (n ¼ 23) eosinophil proteins in sputum macrophages were assessed by quantifying the red hue of sputum macrophages on Romanovsky-stained cytospins as described previously (32).…”

Section: Sputum Processing and Cytokine Assessmentmentioning

confidence: 99%

Elevated Sputum Interleukin-5 and Submucosal Eosinophilia in Obese Individuals with Severe Asthma

Desai

Newby

Symon

et al. 2013

Am J Respir Crit Care Med

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Rationale:The relationship between airway inflammation and obesity in severe asthma is poorly understood. Objectives: We sought to determine the relationship between sputum mediator profiles and the distribution of eosinophilic inflammation and obesity in people with severe asthma. Methods: Clinical parameters and eight mediators in sputum were assessed in 131 subjects with severe asthma from a single center categorized into lean, overweight, and obese groups defined by their body mass index. In an independent group of people with severe asthma (n ¼ 45) and healthy control subjects (n ¼ 19) eosinophilic inflammation was enumerated in bronchial submucosa, blood, and sputum and related to their body mass index. Measurements and Main Results: Sputum IL-5 geometric mean (95% confidence interval) (pg/ml) was elevated in the obese (1.8 [1.2-2.6]) compared with overweight (1.1 [0.8-1.3]; P ¼ 0.025) and lean (0.9 [0.6-1.2]; P ¼ 0.018) subjects with asthma and was correlated with body mass index (r ¼ 0.29; P , 0.001). There was no relationship among body mass index, the sputum cell count, or other sputum mediators. In the bronchoscopy group the submucosal eosinophil number in the subjects with asthma was correlated with body mass index (Spearman rank correlation, r s ¼ 0.38; P ¼ 0.013) and the median (interquartile range) number of submucosal eosinophils was increased in obese (19.4 [11.8-31.2]) (cells per square millimeter) versus lean subjects (8.2 [5.4-14.6]) (P ¼ 0.006). There was no significant association between sputum or peripheral blood eosinophil counts and body mass index. Conclusions: Sputum IL-5 and submucosal eosinophils, but not sputum eosinophils, are elevated in obese people with severe asthma. Whether specific antieosinophilic therapy is beneficial, or improved diet and lifestyle in obese asthma has antiinflammatory effects beyond weight reduction, requires further study.Keywords: asthma; obesity; cytokines; phenotypes; eosinophil Asthma is a common, complex inflammatory disorder affecting about 5% of adults in the general population, of which approximately 5-10% suffer from severe disease (1, 2). Severe disease is often associated with comorbidities, such as obesity, and is particularly important because these patients suffer from substantial morbidity and consume a disproportionately high amount of the overall healthcare resources spent on asthma management (3-6). Asthma, and in particular severe asthma, is a heterogeneous disease as highlighted by the different phenotypes identified using cluster analysis of clinical data (7-10) and cytokine profiles of airway samples (11-13). The key benefit of dividing a multidimensional disease, such as asthma, into distinct phenotypes is expected to be more effective treatment targeting. This has been effectively shown with the success of eosinophilic airway inflammationdirected corticosteroid (14-16) and anti-IL-5 treatment (17-19) to prevent asthma exacerbations in eosinophilic subjects with asthma. The association of obesity and asthma has been evident Support...

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Section: Sputum Processing and Cytokine Assessmentmentioning

confidence: 99%

Elevated Sputum Interleukin-5 and Submucosal Eosinophilia in Obese Individuals with Severe Asthma

Desai

Newby

Symon

et al. 2013

Am J Respir Crit Care Med

Self Cite

202

138

View full text Add to dashboard Cite

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“…In asthma and chronic obstructive pulmonary disease, doctors are distinguishing patients with eosinophilic airway inflammation requiring more aggressive treatment and monitoring [7]; in heart failure, a panel of proteomic tests promises a similar distinction [8].…”

Section: Where Will Pm Arrive First and Where Later?mentioning

confidence: 99%

Is Precision Medicine the Future of Healthcare?

Barker

2017

Per. Med.

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“…Recent data have proved that eosinophil cells actively contribute to the pathogenesis of numerous [20] inflammatory diseases such as asthma and Crohn's disease [21].…”

Section: Medicine Science 2013;2(1):436-44 Eosinophil Cationic Proteimentioning

confidence: 99%

Serum and Amniotic Fluid Eosinophil Cationic Protein Levels in Misoprostol Induced Pregnancies

Çelik¹,

Oztekin²,

Çelik³

et al. 2013

Med-Science

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Eosinophil protein in airway macrophages: A novel biomarker of eosinophilic inflammation in patients with asthma

Abstract: Eosinophil proteins can be reliably measured in airway macrophages. In combination with sputum eosinophilia, the macrophage eosinophil protein content might further define the asthma phenotype and provide an additional tool to direct therapy.

Cited by 73 publications

References 26 publications

Elevated Sputum Interleukin-5 and Submucosal Eosinophilia in Obese Individuals with Severe Asthma

Elevated Sputum Interleukin-5 and Submucosal Eosinophilia in Obese Individuals with Severe Asthma

Is Precision Medicine the Future of Healthcare?

Serum and Amniotic Fluid Eosinophil Cationic Protein Levels in Misoprostol Induced Pregnancies

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