Enzymic conversion of aldrin to dieldrin with subcellular components of pea plants

Lichtenstein, Emanuel P.; Corbett, J. R.

doi:10.1021/jf60163a038

Cited by 24 publications

(18 citation statements)

References 7 publications

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“…Thus, as a supplement to traditional studies with intact plants, in vitro methods seem to have considerable merit. Lichtenstein and Corbett (1969) were able to show only one product (dieldrin) when aldrin was incubated with various fractions of pea root homogenates. The system used did not epoxidize isodrin or heptachlor and did not require NADPH.…”

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confidence: 99%

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Oxidative metabolism of aldrin and isodrin by bean root fractions

Yu¹,

Kiigemagi²,

Terriere³

1971

J. Agric. Food Chem.

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confidence: 99%

“…form many of the pesticide metabolizing reactions exhibited by animal systems. These reactions include hydroxylation (Vaughan and Butt, 1969), M-demethylation (Frear et al, 1969), epoxidation (Lichtenstein and Corbett, 1969), and the "NIH shift" (Russell et al, 1968).…”

mentioning

confidence: 99%

Oxidative metabolism of aldrin and isodrin by bean root fractions

Yu¹,

Kiigemagi²,

Terriere³

1971

J. Agric. Food Chem.

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“…Engelhardt et al (4,5), working with Bacillus sphaericus grown on the phenylurea herbicide linuron, found that these induced cells produced an acylamidase capable of cellfree hydrolysis of the phenylureas, linuron, monolinuron, malaron, and several acylanilides, including the herbicides propanil, karsil, and dicryl. Even the persistent chlorinated hydrocarbons dichlorodiphenyl trichloro ethane (DDT) (2, 11) and aldrin (14) are susceptible to cell-free enzymatic degradation. parathion to p-nitrophenol (PNP) and diethylthiophosphoric acid.…”

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confidence: 99%

Enzymatic hydrolysis of organophosphate insecticides, a possible pesticide disposal method

Munnecke¹

1976

Appl Environ Microbiol

183

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A crude cell extract from a mixed bacterial culture growing on parathion, an organophosphate insecticide, hydrolyzed parathion (21 C) at a rate of 416 nmol/ min per mg of protein. This rate of enzymatic hydrolysis, when compared with chemical hydrolysis by 0.1 N sodium hydroxide at 40 C, was 2,450 times faster. Eight of 12 commonly used organophosphate insecticides were enzymatically hydrolyzed with this enzyme preparation at rates ranging from 12 to 1,360 nmol/ min per mg of protein. Seven pesticides were hydrolyzed at rates significantly higher (40 to 1,005 times faster) than chemical hydrolysis. The pH optimum for enzymatic hydrolysis of the eight pesticides ranged from 8.5 to 9.5, with less than 50%o of maximal activity expressed at pH 7.0. Maximal enzyme activity occurred at 35 C. The crude extract lost its activity at the rate of only 0.75%/day when stored at 6 C. Eight organic solvents, ranging from methanol to hexane, at low concentrations stimulated enzymatic hydrolysis by 3 to 20%, whereas at higher concentrations (1,000 mg/liter) they inhibited the reaction (9 to 50%). Parathion metabolites p-nitrophenol, hydroquinone, and diethylthiophosphoric acid, at up to 100-mg/liter concentrations, did not significantly influence enzyme activity.

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“…The in vitro epoxidation of the organochlorine insecticide, aldrin, to dieldrin was reported by Lichtenstein and Corbett [132] to be mainly associated with the soluble fraction after high speed centrifugation of active pea root homogenates; however, other investigations [133][134][135][136] have localized this epoxidase activity in the particulate fractions of plant root homogenates. The failure of these enzyme preparations to epoxidize the structurally-related insecticide, heptachlor [132][133][134], suggested a narrow substrate specificity.…”

Section: Specific Metabolism In Plantsmentioning

confidence: 94%

“…The failure of these enzyme preparations to epoxidize the structurally-related insecticide, heptachlor [132][133][134], suggested a narrow substrate specificity. Mehendale et al [134] found that dramatically increased levels of enzymatic activity could be observed if insoluble polyvinylpyrrolidone (PVP) was included during root homogenization.…”

Section: Specific Metabolism In Plantsmentioning

confidence: 99%