Enzymes of glycerol and glyceraldehyde metabolism in mouse liver: effects of caloric restriction and age on activities

Hagopian, Kevork; Ramsey, Jon J.; Weindruch, Richard

doi:10.1042/bsr20080015

Cited by 40 publications

(30 citation statements)

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“…Simultaneous operation of these two pentose pathways, however, does not explain the observed rapid growth of S. acidocaldarius on the pentoses. We also observed the upregulation of genes that coded for some components of the glycerol-3-phosphate shuttle pathway (21) in response to glucose. The expression of these glycerol-3-phosphate shuttle pathway genes was likely induced by the glyceraldehyde (or glyceraldehyde-3-phosphate) produced during glycolysis.…”

Section: Discussionmentioning

confidence: 68%

“…Saci_2032 and -2033, which encode glycerol-3-phosphate dehydrogenase and glycerol kinase, respectively, were always upregulated in the presence of glucose, whereas their paralogs (Saci_1117 and -1118) did not respond to the presence of glucose in the growth medium and were induced by xylose (Table 3). These genes are involved in the metabolism of glycerol and glyceraldehyde via the glycerol-3-phosphate pathway (21). Identification of a putative glucose ABC-transport system.…”

Section: Vol 193 2011 Catabolism Of Glucose and Xylose In S Acidocmentioning

confidence: 99%

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Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

et al. 2011

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Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilization. The organism utilized a mixture of 1 g/liter of each sugar simultaneously with a specific growth rate of 0.079 h ؊1 and showed no preference for the order in which it utilized each sugar. The organism grew faster on 2 g/liter xylose (0.074 h ؊1 ) as the sole carbon source than on an equal amount of glucose (0.022 h ؊1 ). When grown on a mixture of the two carbon sources, the growth rate of the organism increased from 0.052 h ؊1 to 0.085 h ؊1 as the ratio of xylose to glucose increased from 0.25 to 4. S. acidocaldarius appeared to utilize a mixture of glucose and xylose at a rate roughly proportional to their concentrations in the medium, resulting in complete utilization of both sugars at about the same time. Gene expression in cells grown on xylose alone was very similar to that in cells grown on a mixture of xylose and glucose and substantially different from that in cells grown on glucose alone. The mechanism by which the organism utilized a mixture of sugars has yet to be elucidated.Sulfolobus acidocaldarius is a hyperthermophilic archaeon that grows optimally at 75°C and pH 2.0 to 3.0 (10, 20, 24) and has been shown to utilize a broad range of sugars (20,24). Numerous studies have shown that bacteria as well as eukaryotes sequentially utilize individual sugars when grown on a mixture of sugars. These organisms preferentially utilize the sugar that best supports their growth (mostly glucose) by repressing the utilization of other sugars in the growth medium until the preferential sugar is completely consumed. This phenomenon, termed "carbon catabolite repression" (CCR), or "diauxie," is characterized by a diphasic growth pattern when an organism is grown on a mixture of glucose and other sugars. Most studies of CCR have focused mainly on bacterial or eukaryotic systems. However, a few studies have reported that the presence of glucose in a growth medium represses the metabolism of other sugars in species closely related to Sulfolobus solfataricus via a mechanism that is similar to CCR (22,23,25,34).S. acidocaldarius metabolizes the smallest number of sugars of all known Sulfolobus species, but the sugars metabolized by the organism include glucose and xylose, the key constituents of ligno-cellulose (cellulosic) biomass (20,24,37). The lack of diauxie on 5-and 6-carbon sugars and the ability to grow at high temperature and low pH are excellent characteristics for a host that produces biofuel from cellulosic biomass deconstructed by acidic and/or high-temperature pretreatment methods. The production of biofuels from cellulosic biomass as an alternative to fossil fuels has received increased attention in the past few years. However, the development of microbial system(s) that can efficiently and...

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Section: Discussionmentioning

confidence: 68%

Section: Vol 193 2011 Catabolism Of Glucose and Xylose In S Acidocmentioning

confidence: 99%

Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

et al. 2011

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“…Increased PEPCK activity has been reported in cultured hepatocytes from fasting mammals (Azzout et al, 1986) and fasting mammalian liver tissues (Hagopian et al, 2008). Gluconeogenesis has been observed in other species of insects post-fasting (Zhou et al, 2004) and it is possible that increased PEPCK transcript accumulation in the mountain pine beetle larvae is induced by experiencing near-starvation during the winter in conjunction with, or even in spite of, declining fall temperatures.…”

Section: Transcript Accumulation Indicates That Glycerol Biosynthesismentioning

confidence: 99%

Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae

Fraser

Bonnett

Keeling

et al. 2016

Preprint

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Winter mortality is a major factor regulating population size of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae).Glycerol is the major cryoprotectant in this freeze intolerant insect. We report findings from a gene expression study on an overwintering mountain pine beetle population over the course of 35 weeks. mRNA transcript levels suggest glycerol production in the mountain pine beetle occurs through glycogenolytic, gluconeogenic and potentially glyceroneogenic pathways, but not from Abbreviations

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“…É utilizado como importante substrato gliconeogênico, principalmente no fígado e nos rins, com sua entrada ocorrendo na forma de gliceraldeído-3-fosfato, ao passo que seu ingresso na via glicolítica se realiza na forma de diidroxiacetona fosfato (HAGOPIAN et al, 2008).…”

Section: Introductionunclassified

Energia digestível e inclusão da glicerina bruta em dietas para juvenis de curimbatá

et al. 2017

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The curimbatá is an important fishery resource, however, little is known about its nutritional requirements. This work aimed to determine the apparent digestibility coefficient (ADC) and the effect of the inclusion of crude glycerin (CG) in the diet for curimbatá (Prochilodus lineatus) juveniles. Feces were collected from fish fed with reference diet based on ingredients of plant origin and a test diet, having the inert marker chromium (III) oxide (0.1%). In the growth experiment, 750 juveniles (18.71 ± 2.70 g) were used in a completely randomized design, with six treatments (diets containing 0, 4, 8, 12, 16, and 20% CG) and five repetitions, for 64 days. For P. lineatus, CG presented ADC of 0.76 for crude energy and digestible energy of 2,850.99 kcal kg -1 of the diet. The different levels of inclusion affected the final weight (FW), feed conversion rate (FCR), specific growth rate (SGR), and weight gain (WG), in addition to blood levels of glucose and triglycerides. Regression analysis showed a negative linear effect of the diets on FW, SGR, and WG, and a positive linear effect on FCR. Crude glycerin can be used as an energy source and its inclusion in 4% of the diet improves the productive performance of curimbatá.Key words: Characiformes; non-protein energy; glycerol; native fish RESUMO O curimbatá é um importante recurso pesqueiro, porém pouco se sabe sobre suas exigências nutricionais. O objetivo deste trabalho foi determinar o coeficiente de digestibilidade aparente (CDA) e o efeito da inclusão da glicerina bruta (GB) em dietas para juvenis do curimbatá Prochilodus lineatus. Foram coletadas fezes de peixes alimentados com dieta-referência, baseada em ingredientes de origem vegetal, e com dieta-teste, tendo o marcador inerte óxido de cromo III (0,1%). No experimento de crescimento foram utilizados 750 juvenis (18,71±2,70 g) em delineamento experimental inteiramente casualizado, com seis tratamentos (dietas contendo 0, 4, 8, 12, 16 e 20% GB) e cinco repetições, durante 64 dias. Para P. lineatus, a GB apresentou CDA de 0,76 para energia bruta e energia digestível de 2.850,99 kcal kg -1 da dieta. Os diferentes níveis de inclusão afetaram o peso final (PFM), conversão alimentar (CAA), taxa de crescimento específico (TCE) e ganho de peso (GPM), além dos níveis sanguíneos de glicose e de triglicerídeos. A análise de regressão mostrou efeito linear negativo das dietas sobre PFM, TCE e GPM e efeito linear positivo sobre CAA. A glicerina bruta pode ser utilizada como fonte de energia e sua inclusão em 4% da dieta promove um melhor desempenho produtivo do curimbatá. Palavras-chave:Characiformes; energia não-proteica; glicerol; peixe nativo.

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Enzymes of glycerol and glyceraldehyde metabolism in mouse liver: effects of caloric restriction and age on activities

Cited by 40 publications

References 64 publications

Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae

Energia digestível e inclusão da glicerina bruta em dietas para juvenis de curimbatá

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Enzymes of glycerol and glyceraldehyde metabolism in mouse liver: effects of caloric restriction and age on activities

Cited by 40 publications

References 64 publications

Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

Absence of Diauxie during Simultaneous Utilization of Glucose and Xylose bySulfolobus acidocaldarius

Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae

Energia digestí­­vel e inclusão da glicerina bruta em dietas para juvenis de curimbatá

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Energia digestível e inclusão da glicerina bruta em dietas para juvenis de curimbatá