Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, <i>Dendroctonus ponderosae</i> Hopkins (Coleoptera: Curculionidae), larvae

Fraser, Jordie D.; Bonnett, Tiffany R.; Keeling, Christopher I.; Huber, Dezene P. W.

doi:10.7287/peerj.preprints.2311v1

Cited by 6 publications

(8 citation statements)

References 38 publications

(57 reference statements)

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“…In addition to markers that have allowed extensive population genetics research, modest genomic resources exist for the investigation of MPB genomic architecture. Draft genomes for both a male and female MPB are available, but the sequences are distributed across 8,188 and 6,520 scaffolds, respectively (Keeling et al, ), and only a few gene families have been annotated (Fraser, Bonnett, Keeling, & Huber, ). Research into MPB gene function is aided by comparisons with resources for related species (McKenna et al, ; Richards et al, ; Vega et al, ), and the MPB genome has considerable synteny with that of the red flour beetle ( Tribolium castaneum Herbst) (Keeling et al, ).…”

Section: Introductionmentioning

confidence: 99%

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Trevoy

Janes

Muirhead

et al. 2018

Ecology and Evolution

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Genetic surveys of the population structure of species can be used as resources for exploring their genomic architecture. By adjusting filtering assumptions, genome‐wide single‐nucleotide polymorphism (SNP) datasets can be reused to give new insights into the genetic basis of divergence and speciation without targeted resampling of specimens. Filtering only for missing data and minor allele frequency, we used a combination of principal components analysis and linkage disequilibrium network analysis to distinguish three cohorts of variable SNPs in the mountain pine beetle in western Canada, including one that was sex‐linked and one that was geographically associated. These marker cohorts indicate genomically localized differentiation, and their detection demonstrates an accessible and intuitive method for discovering potential islands of genomic divergence without a priori knowledge of a species’ genomic architecture. Thus, this method has utility for directly addressing the genomic architecture of species and generating new hypotheses for functional research.

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Section: Introductionmentioning

confidence: 99%

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Trevoy

Janes

Muirhead

et al. 2018

Ecology and Evolution

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“…A critical trait affecting such persistence and expansion is cold‐hardening, which allows overwintering life stages to survive ambient temperatures well below freezing. Cold‐hardening in the MPB involves the production of antifreeze compounds, including glycerol, in response to thermoperiodic cues (Bentz and Mullins 1999, Fraser et al 2017, Thompson et al 2019), dynamic processes that occur with high energetic cost (Danks 1987, Lee 1989). Supercooling points in MPB, indicative of the extent of cold‐hardening, have been shown to differ geographically among populations in the field (Bentz and Mullins 1999), as has been observed in many other insect species with large geographic distributions (Kukal and Duman 1989, Shintani and Ishikawa 2002, Elkinton et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Translocation experiment reveals capacity for mountain pine beetle persistence under climate warming

Soderberg

Mock

Hofstetter

et al. 2020

Ecological Monographs

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Predicting species response to climate change is a central challenge in ecology, particularly for species that inhabit large geographic areas. The mountain pine beetle (MPB) is a significant tree mortality agent in western North America with a distribution limited by climate. Recent warming has caused large‐scale MPB population outbreaks within its historical distribution, in addition to migration northward in western Canada. The relative roles of genetic and environmental sources of variation governing MPB capacity to persist in place in a changing climate, and the migratory potential at its southern range edge in the United States, have not been investigated. We reciprocally translocated MPB populations taken from the core and southern edge of their range, and simultaneously translocated both populations to a warmer, low‐elevation site near the southern range boundary where MPB activity has historically been absent despite suitable hosts. We found genetic variability and extensive plasticity in multiple fitness traits that would allow both populations to persist in a warming climate that resembles the thermal regime of our low‐elevation site. We demonstrate, for the first time, that supercooling points in MPBs are influenced both by genetic and environmental factors. Both populations reproduced with seasonally appropriate univoltine generation times at all translocated sites, and bivoltinism was not observed. The highest reproductive success occurred at the warmest, out‐of‐range low‐elevation site, suggesting that southward migration may not be temperature limited.

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“…Larvae collected from Tête Juane Cache, BC in the autumn of 2008 as part of another study (Fraser, Bonnett, Keeling, & Huber, ) were used for expression studies of IAP . From this collection, we used larvae collected on October 3 and October 10 as these represent the time‐points immediately before and after the temperature dropped below 0°C.…”

Section: Methodsmentioning

confidence: 99%

“…These reference genes were previously identified by Fraser et al. () as effective for normalizing gene expression in D. ponderosae. Each time‐point was used as a separate experiment to account for any temporal changes in expression.…”

Section: Methodsmentioning

confidence: 99%

Selection of the sex‐linked inhibitor of apoptosis in mountain pine beetle (Dendroctonus ponderosae) driven by enhanced expression during early overwintering

Horianopoulos

Boone

Samarasekera

et al. 2018

Ecology and Evolution

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The mountain pine beetle (Dendroctonus ponderosae) is an insect native to western North America; however, its geographical range has recently expanded north in BC and east into Alberta. To understand the population structure in the areas of expansion, 16 gene‐linked microsatellites were screened and compared to neutral microsatellites using outlier analyses of F st and F ct values. One sex‐linked gene, inhibitor of apoptosis (IAP), showed a strong signature of positive selection for neo‐X alleles and was analyzed for evidence of adaptive variation. Alleles of IAP were sequenced, and differences between the neo‐X and neo‐Y alleles were consistent with neutral evolution suggesting that the neo‐Y allele may not be under functional constraints. Neo‐Y alleles were amplified from gDNA, but not effectively from cDNA, suggesting that there was little IAP expression from neo‐Y alleles. There were no differences in overall IAP expression between males and females with the common northern neo‐X allele suggesting that the neo‐X allele in males compensates for the reduced expression of neo‐Y alleles. However, males lacking the most common northern neo‐X allele thought to be selected for in northern populations had reduced overall IAP expression in early October—at a time when beetles are preparing for overwintering. This suggests that the most common allele may have more rapid upregulation. The reduced function of neo‐Y alleles of IAP suggested by both sequence differences and lower levels of expression may foster a highly selective environment for neo‐X alleles such as the common northern allele with more efficient upregulation.

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Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae

Cited by 6 publications

References 38 publications

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle (Dendroctonus ponderosae)

Translocation experiment reveals capacity for mountain pine beetle persistence under climate warming

Selection of the sex‐linked inhibitor of apoptosis in mountain pine beetle (Dendroctonus ponderosae) driven by enhanced expression during early overwintering

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