Enzymes immobilized on montmorillonite K 10: Effect of adsorption and grafting on the surface properties and the enzyme activity

Bioelectroanalytical Determination of Rutin Based on bi‐Enzymatic Sensor Containing Iridium Nanoparticles in Ionic Liquid Phase Supported in Clay

“…This procedure has proven to be more advantageous for catalysis than the use of free enzymes, enabling acceptable control of the procedures, simple isolation of products, and appropriate stability under varying conditions of temperature and pH [24][25][26]. Immobilized enzymes have been efficiently used in food technology, biotechnology, and analytical chemistry, especially in the construction of biosensors.…”

“…MMT possesses excellent intercalation and swelling properties, strong adsorption, and a high affinity for several substances including heavy metal ions and biological materials [19][20][21]. These features make this a suitable support for the immobilization of chemical and biological catalysts, including metallic complexes [21], noble metal NPs [22,23] and enzymes [24].…”

Nanocomposites in Food Packaging – A Review

“…Nanoscale enzyme immobilization systems would have enhanced performance when compared to conventional ones, because of their much higher surface contact area and mass transfer rate, which are probably the most important factors affecting the effectiveness of such systems (Fernández et al, 2008). Approaches might be expected dealing with enzyme adsorption into nanoclays incorporated to polymers (Rhim & Ng, 2007), since nanoclays have a high affinity for protein adsorption, and have been reported to be efficient enzyme carriers (Sinegani et al, 2005;Gopinath & Sugunan, 2007). Conductive polymers may also be used as immobilizing matrices for biomolecules (Ahuja et al, 2007), as reported by Sharma et al (2004), who immobilized glucose oxidase onto films of poly(aniline-co-fluoroaniline).…”

Screening of supports for immobilization of commercial porcine pancreatic lipase

“…In the pillarization process, the basal spacing increases 2 times its value (from 9.0 to 18.6 Å). Comparing the diffractograms of natural and pillared clays, one can note that the peak corresponding to the basal spacing [19][20][21][22][23][24] . Table 2 (a) presents the results in terms of adsorbed protein, yield of immobilization, amount of lipase immobilized and esterification activity for the free and immobilized enzymes using several clays as support.…”

“…The authors reached yields of immobilization of 16 and 55%, respectively. Gopinath and Sugunan 23 and Sanjay and Sugunan 22 immobilized α-amylase, glucoamylase and invertase in montmorillonite K-10 by adsorption and covalent bound techniques. High yields of immobilization were obtained by analysis of nitrogen adsorption isoterms (BET).…”