Enzyme Stabilization by Deposition of Silicone Coatings

Wiemann, Lars O.; Weißhaupt, P.; Nieguth, René; Thum, Oliver; Ansorge‐Schumacher, Marion B.

doi:10.1021/op9000059

Cited by 40 publications

(56 citation statements)

References 11 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[11] Accordingly, product purification, catalyst recovery and recycling were easily accomplished, so proving an excellent applicability of the system from a technical point of view.…”

Section: Increased Stability In Strmentioning

confidence: 94%

“…This is in agreement with the previously reported enhanced stability of silcoat-NZ435 against enzyme leaching. [11] While the native enzyme preparation achieved a half-life time of only about 8 days, silcoat-NZ435 revealed a half-life time up to 63 days. This corresponds to an increase in TTN from 329 g diester /g NZ435 to 2588 g diester /g NZ435 for native NZ435 and silcoat-NZ435, respectively.…”

Section: Application In a More Complex Systemmentioning

confidence: 97%

“…However, severe damage to the enzyme carrier was apparent, which is in agreement with the previously reported disintegration of NZ435 under mechanical stress. [11,12] As a consequence, during catalyst removal the filter was blocked by NZ435 fragments and abrasion, reducing the filtration efficiency and preventing the recovery of catalyst. In the end, a TTN of 100 g MM /g NZ435 could be obtained, a half-life time could not be determined.…”

Section: Increased Stability In Strmentioning

confidence: 99%

See 2 more Smart Citations

Enabling Industrial Biocatalytic Processes by Application of Silicone‐Coated Enzyme Preparations

et al. 2011

View full text Add to dashboard Cite

Composite particles of the commercial lipase preparation NZ435 and silicone (silcoat-NZ435) have recently been described as promising biocatalysts for synthetic use. In this study, their actual potential for enhanced performance in industrial applications was evaluated, focusing on scenarios where carrier disintegration and catalyst leaching constitute key limitations. All three investigated model reactions, the syntheses of myristyl myristate, poly(ethylene glycol) 400-coconut fatty acid monoester and ethylene oxide and propylene oxide co-A C H T U N G T R E N N U N G polymer (EO/PO)-oleic acid diester, were considerably improved in terms of the maximal number of reaction cycles performed with the same batch of catalyst, and consequently in terms of the obtainable product amount. The total turnover numbers (TTN) increased by a factor up to 50, making the realization of this type of reactions in an industrial process more feasible. The utility of silcoat-NZ435 for stereoselective transformations was demonstrated with the enantioselective acylation of 1-phenylethanol with vinyl butyrate, in which full retention of the excellent stereoselectivity of native NZ435 was observed. Moreover, it was demonstrated for the first time that the methodology by which silcoat-catalysts are obtained can be successfully transferred to alternative carriers and enzymes (e.g., protease, esterase and laccase), opening a broad field for the implementation and advancement of biocatalysis in industrial processes.

show abstract

“…[11] Accordingly, product purification, catalyst recovery and recycling were easily accomplished, so proving an excellent applicability of the system from a technical point of view.…”

Section: Increased Stability In Strmentioning

confidence: 94%

Section: Application In a More Complex Systemmentioning

confidence: 97%

Section: Increased Stability In Strmentioning

confidence: 99%

See 1 more Smart Citation

Enabling Industrial Biocatalytic Processes by Application of Silicone‐Coated Enzyme Preparations

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Therefore, as mentioned, enzyme leaching is a known issue with this product. The problem has been addressed by several authors [20,21] . Even though the enzyme performance has been satisfactory, there has been a need specifi cally for a non -leaching immobilized formulation.…”

Section: Immobilizationmentioning

confidence: 99%

Improved Immobilization Supports for Candida Antarctica Lipase B

Saunders

Brask

2010

Biocatalysis in Polymer Chemistry

View full text Add to dashboard Cite

“…Aus diesem Grund eigenen sich Blasensäulen oder Festbettreaktoren besser für Reaktionen mit Enzymträgern, da die Scherbeanspruchung hier geringer ist [1]. Trotzdem wird generell nach Möglichkeiten gesucht, die mechanische Stabilität von Enzymimmobilisaten beispielsweise durch die Beschichtung mit Silikon zu verbessern [2,3].…”

Section: Introductionunclassified

In Situ Microscopy for Online Monitoring of Enzyme Supports and Two‐Phase Systems

Prediger

Bluma

Höpfner

et al. 2011

Chemie Ingenieur Technik

View full text Add to dashboard Cite

In Situ Microscopy for Online Monitoring of Enzyme Supports and Two-Phase SystemsIn situ microscopy (ISM) is an optical analysis method that allows non-invasive gathering of image information from running processes. It is shown how this analysis technique can be used to determine the mechanical stability of enzyme carriers. Furthermore application possibilities of ISM for the characterization of two-phase systems are demonstrated.

show abstract

Enzyme Stabilization by Deposition of Silicone Coatings

Cited by 40 publications

References 11 publications

Enabling Industrial Biocatalytic Processes by Application of Silicone‐Coated Enzyme Preparations

Enabling Industrial Biocatalytic Processes by Application of Silicone‐Coated Enzyme Preparations

Improved Immobilization Supports for Candida Antarctica Lipase B

In Situ Microscopy for Online Monitoring of Enzyme Supports and Two‐Phase Systems

Contact Info

Product

Resources

About