Enabling Industrial Biocatalytic Processes by Application of Silicone‐Coated Enzyme Preparations

Abstract

Composite particles of the commercial lipase preparation NZ435 and silicone (silcoat-NZ435) have recently been described as promising biocatalysts for synthetic use. In this study, their actual potential for enhanced performance in industrial applications was evaluated, focusing on scenarios where carrier disintegration and catalyst leaching constitute key limitations. All three investigated model reactions, the syntheses of myristyl myristate, poly(ethylene glycol) 400-coconut fatty acid monoester and ethylene oxide and propylene oxide co-A C H T U N G T R E N N U N G polymer (EO/PO)-oleic acid diester, were considerably improved in terms of the maximal number of reaction cycles performed with the same batch of catalyst, and consequently in terms of the obtainable product amount. The total turnover numbers (TTN) increased by a factor up to 50, making the realization of this type of reactions in an industrial process more feasible. The utility of silcoat-NZ435 for stereoselective transformations was demonstrated with the enantioselective acylation of 1-phenylethanol with vinyl butyrate, in which full retention of the excellent stereoselectivity of native NZ435 was observed. Moreover, it was demonstrated for the first time that the methodology by which silcoat-catalysts are obtained can be successfully transferred to alternative carriers and enzymes (e.g., protease, esterase and laccase), opening a broad field for the implementation and advancement of biocatalysis in industrial processes.

Hydrophilized Silicone Matrix for the Preparation of Stable Carbonyl Reductase Immobilizates

“…In accordance with the effect of silCoat preparation on NZ435,14, 15 a beneficial effect of HY silCoat formation on the mechanical strength of Fosep F24 was found (Figure 2). Stability against the exerted forces grows exponentially with the increase in the silicone content in the composite particles (Table 6).…”

“…A published example is silCoat ‐NZ435, which is a lipase preparation with considerably improved leaching stability in large‐scale esterification compared to the commercially available NZ435 (Novozymes, Denmark) 14. 15 In addition, the immobilizates show a drastically increased stability of the carrier material against mechanical disintegration.…”

Enzyme Immobilization by Microencapsulation: Methods, Materials, and Technological Applications

“…This method was at first used with lipophilic enzymes such as the lipase preparation NZ435. The enzyme preparation thus obtained showed improved performance in industrial applications in respect to catalyst stability and enzyme leakage as compared to the uncoated enzyme preparation (159,160). Synthesis of myristyl myristate was carried out as a model reaction at lab-scale in a stirred flask reactor, achieving a 23-fold increase of the total turnover number (mass of product produced per mass of enzyme used) compared to the native NZ435.…”

“…Synthesis of myristyl myristate was carried out as a model reaction at lab-scale in a stirred flask reactor, achieving a 23-fold increase of the total turnover number (mass of product produced per mass of enzyme used) compared to the native NZ435. This clearly broadens the range of applications of this lipase, which could be only used efficiently in fixed-bed reactors until then (159).…”

Mit Silikon zum robusten Enzym

“…Dass die Kombination mit Silikon die mechanische Belastbarkeit gängiger Enzymträgermaterialien enorm verbessert, zeigt das Kompositmaterial Silcoat 6. Dieses reduziert gleichzeitig die graduelle Desorption adsorptiv an Träger gebundener Enzyme 7.…”