Enzyme potentiated radioimmunoassay (EPRIA): A sensitive third-generation test for the detection of hepatitis B surface antigen

Ha, Fields; Davis, C. L.; Gr, Dreesman; Dw, Bradley; Je, Maynard

doi:10.1016/0022-1759(81)90115-0

Cited by 8 publications

(1 citation statement)

References 15 publications

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“…Thus, as test conditions and reagents vary, results vary. Although the choice of a constant multiple of the mean absorbance yielded by negative sera to be the threshold for positivity in an ELISA is common (3, 6, 12), some authors have chosen as threshold the mean absorbance of negative controls plus a constant multiple (usually 2, 3, or 5) of the standard deviation (5,14). In theory, this method avoids some of the problems mentioned above; however, unless an impractically large number of wells of negative controls are tested on each plate, the chance variability in the standard deviation is too large to make this method useful (12).…”

Section: Discussionmentioning

confidence: 99%

Comparison of methods for quantitating antigen-specific immunoglobulin M antibody with a reverse enzyme-linked immunosorbent assay

Siegel¹,

Remington²

1983

J Clin Microbiol

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We compared two methods for quantitating antigen-specific antibody by a reverse enzyme-linked immunosorbent assay. In the serial-dilution method, the result was determined to be the highest dilution of a serum yielding an absorbance above an established threshold. In the single-dilution method, the result was determined by comparing the absorbance yielded by the test serum at a standard dilution to that yielded by positive and negative reference sera at the same dilution. The results in the single-dilution method reflected the antigen-specific immunoglobulin M antibody activity as a proportion of total immunoglobulin M antibody in a serum, i.e., the immune load, whereas the results in the serialdilution method reflected the absolute concentration of antigen-specific immunoglobulin M antibody activity. Compared with results in the serial-dilution method, results in the single-dilution method had considerably greater reproducibility on a day-today basis and under various test conditions. The single-dilution method was more useful in discriminating between sera from patients in an early stage of clinical infection due to Toxoplasma gondii and sera from patients in a later stage of infection.

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Section: Discussionmentioning

confidence: 99%