Development of sensitive immunoassays for detection of antibodies against hepatitis B surface antigen

Ionescu-Matiu, Irina; Sanchez, Yanuario; Fields, Howard A.; Dreesman, Gordon R.

doi:10.1016/0166-0934(83)90066-6

Cited by 16 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 1992 survey used SPRIA for HBV infection serologic markers, and the 2006 and 2014 surveys used ELISA. According to previous studies ( 38 , 39 ), SPRIA is less specific than ELISA for detecting HBsAg; this difference could have led to overestimation of the relative decrease in HBsAg prevalence because ELISA testing was used in 2006 and 2014. However, we believe that the effect on our results is modest, especially for the current estimates of HBsAg prevalence, because the ELISA tests used are considered acceptable at international standards.…”

Section: Discussionmentioning

confidence: 99%

Prevention of Chronic Hepatitis B after 3 Decades of Escalating Vaccination Policy, China

Cui¹,

Shen²,

Li³

et al. 2017

Emerg. Infect. Dis.

271

277

View full text Add to dashboard Cite

China’s hepatitis B virus (HBV) prevention policy has been evaluated through nationally representative serologic surveys conducted in 1992 and 2006. We report results of a 2014 serologic survey and reanalysis of the 1992 and 2006 surveys in the context of program policy. The 2014 survey used a 2-stage sample strategy in which townships were selected from 160 longstanding, nationally representative, county-level disease surveillance points, and persons 1–29 years of age were invited to participate. The 2014 sample size was 31,713; the response rate was 83.3%. Compared with the 1992 pre–recombinant vaccine survey, HBV surface antigen prevalence declined 46% by 2006 and by 52% by 2014. Among children <5 years of age, the decline was 97%. China’s HBV prevention program, targeted toward interrupting perinatal transmission, has been highly successful and increasingly effective. However, this progress must be sustained for decades to come, and elimination of HBV transmission will require augmented strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevention of Chronic Hepatitis B after 3 Decades of Escalating Vaccination Policy, China

Cui¹,

Shen²,

Li³

et al. 2017

Emerg. Infect. Dis.

271

277

View full text Add to dashboard Cite

show abstract

“…This step was essential to promote electrostatic bonding between the negatively charged substrate and the NH + site of the antibodies (33). The use of a divalent crosslinker, glutaraldehyde, also helped facilitate the antibody binding mechanism (34,35).…”

Section: Fabrication Of Ito-pani Biosensormentioning

confidence: 99%

Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization

Tahir

Alocilja

Grooms

2007

Sensors

View full text Add to dashboard Cite

Abstract:In this study, a novel indium tin oxide (ITO)-polyaniline (Pani) biosensor was designed, fabricated, and characterized. Initial testing was conducted for the detection of bovine viral diarrhea virus (BVDV). The biosensor design was based upon the specific nature of antibodies to capture the target virus, and the conductive properties of self-doped Pani to translate the antibody-antigen binding into a quantifying signal. The first part of the study was to assess the feasibility of the self-doped Pani to be incorporated into the biosensor design by evaluating its several parameters, such as conductivity, physical structure, thermogravimetric properties, and antibody-binding properties. The second part of the paper highlights the fabrication of the ITO-Pani biosensor to detect the presence of bovine viral diarrhea virus (BVDV) in pure culture. Although only BVDV culture was tested in this study, the biosensor is versatile for the detection of other pathogen of interest by changing the specificity of the antibodies.

show abstract

Label-Free Microbial Biosensors Using Molecular Nanowire Transducers

Alocilja

Muhammad-Tahir

Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems

View full text Add to dashboard Cite

Development of sensitive immunoassays for detection of antibodies against hepatitis B surface antigen

Cited by 16 publications

References 22 publications

Prevention of Chronic Hepatitis B after 3 Decades of Escalating Vaccination Policy, China

Prevention of Chronic Hepatitis B after 3 Decades of Escalating Vaccination Policy, China

Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization

Label-Free Microbial Biosensors Using Molecular Nanowire Transducers

Contact Info

Product

Resources

About