Enzyme measurements by mass: an interim review of the clinical efficacy of some mass measurements of prostatic acid phosphatase and the isoenzymes of creatine kinase

Henderson, A R; Nealon, D A

doi:10.1016/0009-8981(81)90103-0

Cited by 8 publications

(3 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination, however, of CK activity in human seminal plasma seems to be of limited diagnostic value for male infertility (Gonzales- Buitrago et aI., 1980), while measurement of BB-CK activity in seminal plasma, prostatic fluid or urine has been suggested to be a valuable diagnostic tool for prostatic carcinoma (Chapman et al, 1980;Henderson & Nealon, 1981;Edwards et al, 1982). Based on our results we believe that the determination of CK activity of isolated spermatozoa as opposed to seminal plasma may be of more clinical potential in the diagnosis of male infertility.…”

Section: Discussionmentioning

confidence: 97%

Creatine kinase isoenzymes in spermatozoa

Wallimann

Moser

Zurbriggen

et al. 1986

J Muscle Res Cell Motil

View full text Add to dashboard Cite

Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized by indirect immunofluorescence staining within the sperm tail but not in the head portion. MiMi-CK is confined to the midpiece region rich in mitochondria and has been localized directly by immunogold staining within the mitochondria. In contrast to chicken, seminal plasma from man was also found to contain considerable amounts of BB-CK. Total creatine content of spermatozoa (8-15 mM) and seminal plasma (3.8 +/- 0.4 mM) as well as preliminary experiments with metabolic blockers indicate a dependence of sperm motility on CK and phosphoryl creatine (CP). The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle.

show abstract

Section: Discussionmentioning

confidence: 97%

Creatine kinase isoenzymes in spermatozoa

Wallimann

Moser

Zurbriggen

et al. 1986

J Muscle Res Cell Motil

View full text Add to dashboard Cite

show abstract

“…Consideration of this assay suggests that significant levels of ATPase could seriously interfere with creatine kinase estimation by this method. Observations of raised serum levels of creatine kinase (BB form) in men with prostatic cancer (reviewed by Henderson & Nealon, 1981) suggested that creatine kinase assays of prostatic fluid could be clinically useful in the diagnosis of prostatic carcinoma. We have therefore con¬ sidered various assays for creatine kinase activity.…”

Section: Introductionmentioning

confidence: 99%

Creatine kinase and ATPase in human seminal fluid and prostatic fluid

Kavanagh¹,

Darby²

1983

Reproduction

View full text Add to dashboard Cite

A creatine kinase assay based on estimation of creatine liberated from creatine phosphate was accurate and reproducible for use with seminal or prostatic fluid, after allowance was made for acid phosphatase interference. Comparison of this method with one which relies on enzymic coupling of ATP formation to NADP+ oxidation shows that the latter under-estimates creatine kinase activity by a factor of about 3. This discrepancy could be due to the high ATPase activity found in prostatic and seminal fluid. Uncritical use of the NADP+ assay might account for different seminal creatine kinase values reported in the literature.Interrelationships between ATPase, creatine kinase and zinc suggest that seminal ATPase is a prostatic secretory product while creatine kinase may be multiglandular in origin.

show abstract

“…This may reflect differences in antigen preparation since one of the most meticulously prepared antigens gave one of the lower decision thresholds. 44 The most recent publication utilizing the only commercially available monoclonal IRMA assay once again raises hopes of better diagnostic sensitivity at Stage A, PAC. The authors found 4/9 (44%) of the patients to be above the decision threshold by Tandem assay, but of the same 9 , only 2 (22%) were above this limit by RIA.…”

Section: E Comparison Of Pacp Methods In Prostatic Diseasementioning

confidence: 99%

The Laboratory Diagnosis of Prostatic Adenocarcinoma

1983

CRC Critical Reviews in Clinical Laboratory Sciences

View full text Add to dashboard Cite

At the close of each decade, we are reminded by medical statisticians that our longevity increases significantly. For the male, especially if he be of black race this statement has an ironic twist. Over age 70, prostatic "cancer" assumes the leadership list of "cancers" in general, supplanting lung and colo-rectal. One interesting point evolved by careful autopsy studies suggests that this incidence is found coincidentally and is not primarily responsible for the cause of death. This illustrates a different significance to other neoplasia, and offers useful opportunities to study the evolution of a neoplasm. In contrast to other "cancers" (for example pulmonary), the histological nature of the tumor is almost totally derived from the acinar lobules and designated adenocarcinoma. Neoplasia arising from the fibromuscular stroma (sarcoma) and metaplastic ductus (squamous cell carcinoma) constitute less than 1% of all prostate cancers. Histological appearances, however, are not as simple as hoped. As in many tumors the section may present a uniform well-differentiated adenocarcinoma in which the acinar structure is well maintained--yet at the opposing end of the spectrum show a fatally dedifferentiated picture whose organ origin is difficult to determine. Adding to the complication is the wide variation, far more commonly seen, of the mixed tumor with all variations presenting a composite panorama of histology. Indeed the pure type is rare. As with all neoplastic disease, early detection is critical, since opportunity for cure with the various forms of therapy from surgery through radiation to chemotherapy are increasing rapidly. The prostate gland is relatively accessible to the trained finger of the physician and later stages of the disease are palpable. However, the earliest Stage (I) is not discovered by rectal examination, hence provides an ideal opportunity for the serum tumor marker, to identify disease. Since 1938, disseminated prostatic adenocarcinoma has been associated with elevation of activity of an enzyme acid phosphatase. Although there are several isoenzymes the prostatic specific one has in the past been assayed by different spectrophotometric techniques using selective substrate and chemical inhibition. More recently various immunological methods have added a greater sensitivity and specificity to the early detection of prostatic adenocarcinoma. However is should be clearly stated that prostatic acid phosphatase is not cancer specific and can be associated with other nonmalignant lesions in the prostate gland.(ABSTRACT TRUNCATED AT 400 WORDS)

show abstract

Enzyme measurements by mass: an interim review of the clinical efficacy of some mass measurements of prostatic acid phosphatase and the isoenzymes of creatine kinase

Cited by 8 publications

References 66 publications

Creatine kinase isoenzymes in spermatozoa

Creatine kinase isoenzymes in spermatozoa

Creatine kinase and ATPase in human seminal fluid and prostatic fluid

The Laboratory Diagnosis of Prostatic Adenocarcinoma

Contact Info

Product

Resources

About