Creatine kinase and ATPase in human seminal fluid and prostatic fluid

Kavanagh, John P.; Darby, Charles P.

doi:10.1530/jrf.0.0680051

Cited by 22 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, BB-CK (located throughout the sperm tail), generates the required ATP from this CP and ADP. In contrast to seminal plasma from chicken which does not contain any CK, seminal plasma from man was found to contain significant amounts of BB-CK, the origin of which has been suggested to be multiglandular, namely ~rom the prostatic gland (Kavanagh & Darby, 1983) and the seminal vesicle (Soufir, 1979). It should be pointed out here that hens after mating can store spermatozoa over long periods of time and release them when needed for fertilization of the eggs; the lack of an additional external CK supply in seminal fluid of roosters may be related to this physiological speciality.…”

Section: Discussionmentioning

confidence: 93%

“…While considerable data on the presence of brain-type creatine kinase (BB-CK) in semen of man has already been documented and its multiglandular origin from the prostate and the seminal vesicle postulated (Kavanagh & Darby, 1983), little is known about the presence of this enzyme within spermatozoa. The only report on the presence of some CK activity in spermatozoa, incidentally from roosters, was published by Buckland (1970).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Creatine kinase isoenzymes in spermatozoa

Wallimann

Moser

Zurbriggen

et al. 1986

J Muscle Res Cell Motil

View full text Add to dashboard Cite

Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized by indirect immunofluorescence staining within the sperm tail but not in the head portion. MiMi-CK is confined to the midpiece region rich in mitochondria and has been localized directly by immunogold staining within the mitochondria. In contrast to chicken, seminal plasma from man was also found to contain considerable amounts of BB-CK. Total creatine content of spermatozoa (8-15 mM) and seminal plasma (3.8 +/- 0.4 mM) as well as preliminary experiments with metabolic blockers indicate a dependence of sperm motility on CK and phosphoryl creatine (CP). The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Creatine kinase isoenzymes in spermatozoa

Wallimann

Moser

Zurbriggen

et al. 1986

J Muscle Res Cell Motil

View full text Add to dashboard Cite

show abstract

“…Creatine kinase (E.C.2.7.3.2) catalyses the reversible phosphorylation of ADP to ATP or creatine to creatine phosphate, thus maintaining an immediately accessible energy reservoir in the cell 16. Creatine phosphokinase, which is a key enzyme in transport and energy synthesis, supplies ATP to the sperm 17. Sperm are frequently associated with elevated activities of certain key enzymes, including creatine kinase, which indicate male infertility 18-20…”

Section: Introductionmentioning

confidence: 99%

The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field

Kumar

Kesari

Behari

2011

Clinics

View full text Add to dashboard Cite

INTRODUCTION:Environmental exposure to man-made electromagnetic fields has been steadily increasing with the growing demand for electronic items that are operational at various frequencies. Testicular function is particularly susceptible to radiation emitted by electromagnetic fields.OBJECTIVES:This study aimed to examine the therapeutic effects of a pulsed electromagnetic field (100 Hz) on the reproductive systems of male Wistar rats (70 days old).METHODS:The experiments were divided into five groups: microwave sham, microwave exposure (2.45 GHz), pulsed electromagnetic field sham, pulsed electromagnetic field (100 Hz) exposure, and microwave/pulsed electromagnetic field exposure. The animals were exposed for 2 hours/day for 60 days. After exposure, the animals were sacrificed, their sperm was used for creatine and caspase assays, and their serum was used for melatonin and testosterone assays.RESULTS:The results showed significant increases in caspase and creatine kinase and significant decreases in testosterone and melatonin in the exposed groups. This finding emphasizes that reactive oxygen species (a potential inducer of cancer) are the primary cause of DNA damage. However, pulsed electromagnetic field exposure relieves the effect of microwave exposure by inducing Faraday currents.CONCLUSIONS:Electromagnetic fields are recognized as hazards that affect testicular function by generating reactive oxygen species and reduce the bioavailability of androgen to maturing spermatozoa. Thus, microwave exposure adversely affects male fertility, whereas pulsed electromagnetic field therapy is a non-invasive, simple technique that can be used as a scavenger agent to combat oxidative stress.

show abstract

“…Human seminal plasma contains a nucleoside triphosphate phosphohydrolase, the preferred substrate of which is ATP, in rather large quantities [1][2][3][4][5][6]. This ATPase was obligatorily dependent on Mg2+ and/or Ca2+ for activity and ouabain was inefficient as an inhibitor [2].…”

Section: Introductionmentioning

confidence: 99%

“…This ATPase was obligatorily dependent on Mg2+ and/or Ca2+ for activity and ouabain was inefficient as an inhibitor [2]. The ATPase system was intimately linked to membranes encasing small secretory granules and vesicles of prostatic origin [2,4,5]. These organelles, which apparently occurred freely in human seminal plasma under 'physiological' conditions, were later denoted 'prostasomes' [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of modulators on prostasome membrane‐bound ATPase in human seminal plasma

Ronquist

1987

Eur J Clin Investigation

View full text Add to dashboard Cite

The Mg2+- and Ca2+-dependent ATPase system in human seminal fluid is linked to membranes encasing small organelles denoted 'prostasomes'. This activity was completely pelleted after ultracentrifugation at 105,000 X g, provided the seminal plasma was diluted 1:10. On the contrary, prostatic acid phosphatase activity remained in the supernatant and was inhibited to about 75% by tartrate (0.5 mmol l-1) contrasting with the ATPase system that was not inhibited. Calmodulin, in the concentration interval 0.30-0.90 mumol l-1, did not further activate the ATPase system. Calmidazolium, which is a highly lipophilic substance, was a competitive inhibitor of the Mg2+- and Ca2+-dependent ATPase and half maximal inhibition was attained at 2-3 mumol l-1 calmidazolium. This inhibition was antagonized to a moderate degree (25%, P less than 0.02) by calmodulin, 0.60 mumol l-1. Quercetin, at concentrations of 30-60 mumol l-1, did not influence the prostasome membrane ATPase in any direction. Oleate was an inhibitor of the ATPase system at any concentration up to 50 mumol l-1. This inhibition was not counteracted to an appreciable extent by calmodulin. These results displayed a pattern giving support to the view that the Mg2+- and Ca2+-dependent ATPase of the prostasome membrane may be the molecular basis for vectorial transport of Ca2+ into prostasomes.

show abstract

Creatine kinase and ATPase in human seminal fluid and prostatic fluid

Cited by 22 publications

References 10 publications

Creatine kinase isoenzymes in spermatozoa

Creatine kinase isoenzymes in spermatozoa

The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field

Effect of modulators on prostasome membrane‐bound ATPase in human seminal plasma

Contact Info

Product

Resources

About