Enzyme-linked immunosorbent assay utilizing thin-layered microfluidics

Abstract

An antibody-immobilized thin-layered glass microfluidic channel with a high surface-to-volume ratio was developed for rapid and sensitive enzyme-linked immunosorbent assay.

Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein

“…This coincides with the data obtained in previous work for a channel with a depth of 5.2 mm. 23 The parameters of analyte injection were selected based on these data. Those parameters were 35 and 20 s for 2.0 and 5.2 mm depth channels, which correspond to 0.08 and 0.2 mL of sample volume, respectively.…”

“…The previous unoptimized parameters were 0.1 mg mL −1 of HRP and 60 s of reaction time. 23 To conrm efficient surface recovery and reproducibility of the assay, we measured the signal both with and without antigen (CRP) over several repetition cycles (Fig. 4).…”

“…This protein has a well-established ELISA protocol for its detection, for which we used a non-competitive sandwich mode of immunoassay. In previous work 23 we studied the proof-ofconcept established for thin-layered ELISA and conrmed a uidic control in the introduction of the sample. Also, we veried the working principle of thin-layered ELISA by measuring a standard CRP solution and evaluated the detection performance.…”

AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

“…For example, the uorescent signal is oen disturbed by environment and suffered from undesirable photobleaching or self-uorescence, 15 while the enzyme or chemiluminescence reactions usually time consuming, require special substrates and have certain harm to environment. [16][17][18] Surface enhanced Raman scattering (SERS) is a fast, stable and highly sensitive spectroscopic technology, which has led to great achievements in environmental monitoring, food safety supervision and biological imaging. [19][20][21] Upon coupling SERS with immunoassay, the developed SERS-immunoassay method expanded the analyte scope, and thus attracted growing interest in medicine diagnosis.…”

Improved Size Uniformity of Microcapillaries in Microcapillary Films by Separate Gas Injection

“…First reported by Hallmark et al in 2005, [1] MCFs DOI: 10.1002/mame.202100499 have been employed in various applications such as microreactions, [2][3][4] micromixing, [5] droplet generation, [6] crystal generation, [7] nanoparticle capture, [8][9][10] and bioanalytical technologies. [11][12][13][14][15] Several kinds of polymers have been used to produce MCFs, including ethylene vinyl alcohol, [16] thermoplastic polyurethane, [5] polyethylene, [17] polylactic acid, [18] and fluorinated ethylene propylene. [19] MCFs can be produced with different numbers of microcapillaries, such as 7, 10, [20] 19, [21] and 28.…”