Enzyme-linked immunosorbent assay using F(ab′)2 fragment for the detection of human pulmonary surfactant protein D in sera

Pulmonary Collectins Protect Macrophages against Pore-forming Activity of Legionella pneumophila and Suppress Its Intracellular Growth

“…Monoclonal antibodies were prepared and characterized as described previously (25,26). The bacterial suspension (10 6 CFU in 40 l of PBS/well) was put into the microtiter wells (Immulon 1B, Thermo) and dried under UV irradiation.…”

Serum surfactant proteins-A and -D as biomarkers in idiopathic pulmonary fibrosis

“…Human SP-D was measured by ELISA, which has been described previously [15]. Recombinant SP-D was used as the standard for this assay, as previously described [16].…”

Surfactant protein D in serum from patients with allergic bronchopulmonary aspergillosis

“…The concentration of SP-D in serum was assessed with an established enzyme-linked-immunosorbent-assay validated for measurements in serum (Yamasa Corporation, Choshicity, Japan) [11].…”