Enzyme-linked immunosorbent assay of epidermal growth factor receptor in lung cancer: comparisons with immunohistochemistry, clinicopathological features and prognosis

Abstract

Summary The prognostic role of epidermal growth factor receptor (EGFR)

Clinical significance of biological differences between cavitated and solid form of squamous cell lung cancer

“…In the literature, there is a disagreement about prognostic significance of EGFR expression in NSCLC patients. In most studies, EGFR expression was not correlated with patients' survival [20,22,23,30,32,33]. However, some authors found EGFR overexpression as a positive [34] and some as a negative [24] factor influencing patients' survival.…”

“…For example, data on the significance of tumour proliferation rate in NSCLC are controversial [15][16][17][18][19]. The same is true about the epidermal growth factor (EGF) [20][21][22][23][24]. It has become apparent that cancer cells are able to produce and respond to their own growth factors.…”

Prognostic significance of EGFR and Her-2 in oral cavity cancer in betel quid prevalent area

“…Although the ELISA technique is less used in clinical study, this method is also widely accepted to determine protein expression levels. Examples include using this technique in the studies of breast cancer, cervical cancer, lung cancer as well as head and neck cancer (Christensen et al, 1995;Pfeiffer et al, 1998;Contreras et al, 2002;Widschwendter et al, 2002). The key advantage of the ELISA method is to provide a quantitative result with relatively less bias.…”

“…The key advantage of the ELISA method is to provide a quantitative result with relatively less bias. When comparing these two methods, Pfeiffer et al (1998) have reported that significant correlation was found in the quantification of EGFR or Her-2 levels between ELISA and immunohistochemical methods studied in the lung and in breast cancers. These studies suggest that comparable evaluation results may be obtained by using these two assay techniques.…”

Activation state egfr and STAT-3 as prognostic markers in resected non-small cell lung cancer

“…However, total EGFR expression rarely relates to other clinical prognostic indicators in NSCLC [10]. Only 3 of 10 published studies have shown an association between EGFR expression and either relapse-free or overall survival rates [11][12][13][14][15][16][17][18][19][20]. Importantly studies have shown that total EGFR expression does not correlate with clinical response to tyrosine kinase inhibitors like gefitinib or erlotinib [21] suggesting other characteristics of EGFR beside expression level are important to the response.…”