Enzyme-linked immunosorbent assay for myosin heavy chains in the horse

Barrey, Éric; Valette, Jean-Paul; Jouglin, Maggy; Picard, Brigitte; Geay, Y.; Robelin, J.

doi:10.1051/rnd:19950602

Cited by 11 publications

(11 citation statements)

References 22 publications

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“…SDS-PAGE analysis of histochemically pretyped single fibers was used in that study. Similar observations were reported in two more recent studies of equine skeletal muscles by using a 5-8% SDS-PAGE separating gel with 25-40% glycerol (Yamaguchi et al, 1993;Barrey et al, 1995). In the latter study, it was concluded that an enzyme-linked immunoassay method made it possible to measure a wide range of MyHC contents in equine muscles, but the technique only used two complementary monoclonal antibodies specific against slow and all fast MyHC isoforms.…”

Section: Discussionsupporting

confidence: 78%

“…Some studies have investigated the composition of MyHC isoforms in equine skeletal muscle by using immunohistochemistry (Snow et al, 1981;Sinha et al, 1992) or gel electrophoresis (Billeter et al, 1987;Sosnicki et al, 1989;Yamaguchi et al, 1993;Barrey et al, 1995) or a combination of both methods (Hermanson et al, 1991;Cobb et al, 1994). The content of MyHCs of five equine muscles has also been investigated by using enzyme-linked immunosorbent assay (Barrey et al, 1995).…”

mentioning

confidence: 99%

“…The content of MyHCs of five equine muscles has also been investigated by using enzyme-linked immunosorbent assay (Barrey et al, 1995). All of these investigations distinguished two different MyHCs: slow and fast.…”

mentioning

confidence: 99%

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Myosin heavy chain isoforms in adult equine skeletal muscle: An immunohistochemical and electrophoretic study

1996

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Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 99%

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Myosin heavy chain isoforms in adult equine skeletal muscle: An immunohistochemical and electrophoretic study

1996

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“…In the semitendinosus muscle we found a steady 13% type I fibres. Barrey et al (1995) found mean 2% type I fibres. On the other hand, Essén et al (1980) detected 12-15% and some investigators (Aberle et al 1976) an even higher percentage type I fibres (up to 30%).…”

Section: Fibre Type Composition Of the Gluteus Medius And Semitendinomentioning

confidence: 89%

Changes in fibre type composition of gluteus medius and semitendinosus muscles of Dutch Warmblood foals and the effect of exercise during the first year postpartum

Dingboom

Oudheusden

Eizema

et al. 2002

Equine Veterinary Journal

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Summary In order to obtain broader insights into the equine musculoskeletal system, we studied the fibre type composition of 2 locomotory muscles in biopsies from Dutch Warmblood foals taken at 3 different ages in the first year postpartum. The muscle fibre types were determined histochemically as well as immunohistochemically. ATPase‐characterised IIB fibres appear to express either IId or type IIa plus IId myosin heavy chain (MHC). A high percentage of fibres classified as IIA with ATPase expressed both fast types of MHC. The type I classification by the 2 methods matched almost completely. There was an increase with age of fibres expressing I and IIa MHC in the gluteus medius. At the same time, there was a decrease of fibres expressing IId MHC and fibres co‐expressing MHC IIa and IId. MHC expression of the semitendinosus muscle did not change over time at first, but from age 22–48 weeks there was a decrease in the percentage of type IId fibres. In general, the gluteus medius contained more type I fibres but fewer type IId fibres compared to the semitendinosus. At most ages the fibre type compositions of both muscles correlated with one another. To examine the effect of exercise, one‐third of the foals were given box rest, one‐third received training and one‐third kept at pasture during the first 22 weeks of life. The 3 exercise groups differed in their fibre type composition; however, these differences could not be attributed to the effect of exercise.

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“…A 10-20 mg muscle sample was collected from each compartment and immediately frozen into liquid nitrogen until analysis. For each sample, the percentages of slow (MHC 1) and of the 2 fast myosin heavy chains isoforms (fast MHCs) were measured by using 2 specific monoclonal antibodies (MAb 1, ref F36-5B92; MAb 2, ref NCL-MHCf3) with an enzymatic linked immunoassay (ELISA) method as previously described by Barrey et al (1995).…”

Section: Muscular Biopsy Technique and Myosin Heavy Chains Analysismentioning

confidence: 99%

Standardisation of muscular biopsy ofgluteus mediusin French trotters

Valette

Barrey

Jouglin

et al. 1999

Equine Veterinary Journal

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Summary Morphometric measurements were taken from 41 French trotters of various ages and both sexes. Biopsy location was determined for the dorsal compartment as being one‐third of the distance from the tuber sacrale to the tuber coxae and for the ventral compartment as being one‐third of the distance from the tuber coxae to the caudal Cd1‐Cd2 intervertebral joint. Ten horses were biopsied at these 2 sites at a sampling depth equal to half the total depth of the compartment as measured by ultrasonography. The percentage of slow and fast myosin heavy chain fibres was measured by using an immunoenzymatic method. The depth of the dorsal and ventral compartments of the gluteus medius was significantly greater in males than in females. The depth of the ventral compartment was greater in the case of a straight hip than of a wide hip and was greater in young horses than in older horses. The ventral and the dorsal compartments were composed in the mid‐portion of 81.3 and 75.6% of fast myosin heavy chains, respectively. It was concluded that the locations and sampling depths in the gluteus medius could be standardised in French trotters by taking into account anatomical references, sex, age and width of the hip.

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Enzyme-linked immunosorbent assay for myosin heavy chains in the horse

Cited by 11 publications

References 22 publications

Myosin heavy chain isoforms in adult equine skeletal muscle: An immunohistochemical and electrophoretic study

Myosin heavy chain isoforms in adult equine skeletal muscle: An immunohistochemical and electrophoretic study

Changes in fibre type composition of gluteus medius and semitendinosus muscles of Dutch Warmblood foals and the effect of exercise during the first year postpartum

Standardisation of muscular biopsy ofgluteus mediusin French trotters

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