Standardisation of muscular biopsy ofgluteus mediusin French trotters

Abstract

Summary Morphometric measurements were taken from 41 French trotters of various ages and both sexes. Biopsy location was determined for the dorsal compartment as being one‐third of the distance from the tuber sacrale to the tuber coxae and for the ventral compartment as being one‐third of the distance from the tuber coxae to the caudal Cd1‐Cd2 intervertebral joint. Ten horses were biopsied at these 2 sites at a sampling depth equal to half the total depth of the compartment as measured by ultrasonography. The percentage of slow and fast myosin heavy chain fibres was measured by using an immunoenzymatic method. The depth of the dorsal and ventral compartments of the gluteus medius was significantly greater in males than in females. The depth of the ventral compartment was greater in the case of a straight hip than of a wide hip and was greater in young horses than in older horses. The ventral and the dorsal compartments were composed in the mid‐portion of 81.3 and 75.6% of fast myosin heavy chains, respectively. It was concluded that the locations and sampling depths in the gluteus medius could be standardised in French trotters by taking into account anatomical references, sex, age and width of the hip.

Effects of repeated biopsying on muscle tissue in horses

“…The importance of exact standardisation of biopsying for depth, site and anatomical compartment of the equine gluteus muscle has been stressed previously (Bruce and Turek 1985;Kline et al 1 9 8 7 ;Wood et al 1988;Rivero et al 1992Rivero et al , 1993Bruce et al 1 9 9 3 ;Linnane et al 1999;Valette et al 1999). The results of the present study showed, additionally, that for samples only 3 cm apart laterally there were large differences between muscle variables, especially for the samples taken at 6 cm depth.…”

Transcriptome analysis of muscle in horses suffering from recurrent exertional rhabdomyolysis revealed energetic pathway alterations and disruption in the cytosolic calcium regulation

“…All horses were sedated, and local anaesthesia was performed before making a small skin incision. The dorsal compartment of the gluteus medius biopsy was performed at the first third distance between the sacral and coxae tuber (Valette et al. 1999).…”

Muscular microRNA expressions in healthy and myopathic horses suffering from polysaccharide storage myopathy or recurrent exertional rhabdomyolysis

“…2008) or RER disease (Valberg et al . 1993) and (iii) the good standardisation of biopsy sampling (Valette et al . 1999).…”

“…For each horse, a microbiopsy of the gluteus medius muscle was performed under local anaesthesia. This muscle was chosen because of: (i) its important propulsion function in horse locomotion, (ii) its early and major histological involvement in the course of PSSM (Larcher et al 2008) or RER disease (Valberg et al 1993) and (iii) the good standardisation of biopsy sampling (Valette et al 1999). The horses were sedated and received a local anaesthesic before a small skin incision was made according to a standardised protocol used routinely for muscle disease diagnostis.…”