Enzyme-linked immunosorbent assay for diagnosis of brucellosis

Saz, José Vicente; Beltrán, María; Díaz, Ana Carolina; Agulla, A; Merino, F.; Villasante, P A; Velasco, A.

doi:10.1007/bf02097200

Cited by 22 publications

(14 citation statements)

References 11 publications

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“…According to recent studies by Smits et al (35), 11% of patients with brucellosis do not have detectable levels of specific IgM by immunoblot analysis. Similar results were found with enzyme immunoassay techniques (3,4,6,34). In these cases, seroconversion accompanied by a significant rise in antibody titer allows brucellosis to be diagnosed.…”

Section: Discussionsupporting

confidence: 78%

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Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

Orduña¹,

Almaraz²,

Prado³

et al. 2000

J Clin Microbiol

113

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We evaluated the validity and the usefulness of a new test for the diagnosis of human brucellosis based on an immunocapture-agglutination technique. A total of 315 sera from 82 patients with a diagnosis of brucellosis, 157 sera from patients in whom brucellosis was suspected but not confirmed, and 412 sera from people living in rural areas with endemic brucellosis were studied. The seroagglutination test (SAT), Coombs anti-Brucella test, and Brucellacapt test were evaluated. All the initial sera from the 82 patients proved to be positive in Brucellacapt and Coombs tests, while only 75 (91.4%) were positive in the SAT. If a ≥1/160 diagnostic threshold titer was defined for the Brucellacapt test, Coombs test, and SAT, the sensitivities were 95.1, 91.5, and 65.8%, respectively. Taking the same diagnostic threshold titer for the 157 sera from the unconfirmed but suspected patients, the specificities of the Brucellacapt, Coombs, and SAT were 81.5, 96.2, and 100%, respectively; for the 412 control sera, the specificities were 99.0, 99.8, and 100%. The diagnostic efficiency (area below the receiver operating characteristic curve) of Brucellacapt was 0.987852 (95% confidence interval [CI], 0.95109 to 0.99286), very similar to the diagnostic efficiency of the Coombs test (0.97611; 95% CI, 0.94781 to 0.99146) and higher than that of SAT (0.91013; 95% CI, 0.86649 to 0.94317). The results of the Brucellacapt test were compared with those of the Coombs test (correlation coefficient, 0.956; P= 0.000) and SAT (correlation coefficient, 0.866; P = 0.000). The study shows very good correlation between the Brucellacapt and Coombs tests, with a high concordance between titers obtained in the two tests. Nevertheless, lower correlation and concordance were found between the Brucellacapt and Coombs tests when the results for titers of ≥1/160 were compared (0.692; P = 0.000). In acute brucellosis, the Brucellacapt and Coombs tests render positive titers of ≥1/160. When the titers are lower, they increase significantly in the following 30 days, despite the evolution of SAT titers. In contrast, Brucellacapt and Coombs titers are always high (≥1/640) in brucellosis with long evolution, whether SAT titers are higher or lower than 1/160.

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Section: Discussionsupporting

confidence: 78%

“…The most commonly used tests are the serum agglutination test (SAT), the Coombs anti-Brucella test, the Rose Bengal test, and complement fixation. During the last decade, radioimmunoassay (24,28) and enzyme immunoassay (6,21,34) tests have also been used. These present technical difficulties since they require skilled personnel and high-cost material.…”

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confidence: 99%

Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

Orduña¹,

Almaraz²,

Prado³

et al. 2000

J Clin Microbiol

113

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“…In 13 of the 16 studies included in this review, standard RBT performance was compared to culture as the reference test [ 2 , 15 , 19 – 21 , 23 , 27 , 28 , 30 – 32 ], and in 3/16 studies [ 2 , 23 , 34 ], titrated RBT (involving serum dilutions made in phosphate-buffered saline and then tested with an equal volume of the RBT reagent) was compared to culture ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Utility of the Rose Bengal Test as a Point-of-Care Test for Human Brucellosis in Endemic African Settings: A Systematic Review

Ekiri

Kilonzo

Bird

et al. 2020

Journal of Tropical Medicine

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In endemic African areas, such as Tanzania, Brucella spp. cause human febrile illnesses, which often go unrecognized and misdiagnosed, resulting in delayed diagnosis, underdiagnosis, and underreporting. Although rapid and affordable point-of-care tests, such as the Rose Bengal test (RBT), are available, acceptance and adoption of these tests at the national level are hindered by a lack of local diagnostic performance data. To address this need, evidence on the diagnostic performance of RBT as a human brucellosis point-of-care test was reviewed. The review was initially focused on studies conducted in Tanzania but was later extended to worldwide because few relevant studies from Tanzania were identified. Databases including Web of Science, Embase, MEDLINE, and World Health Organization Global Index Medicus were searched for studies assessing the diagnostic performance of RBT (sensitivity and specificity) for detection of human brucellosis, in comparison to the reference standard culture. Sixteen eligible studies were identified and reviewed following screening. The diagnostic sensitivity (DSe) and specificity (DSp) of RBT compared to culture as the gold standard were 87.5% and 100%, respectively, in studies that used suitable “true positive” and “true negative” patient comparison groups and were considered to be of high scientific quality. Diagnostic DSe and DSp of RBT compared to culture in studies that also used suitable “true positive” and “true negative” patient comparison groups but were considered to be of moderate scientific quality varied from 92.5% to 100% and 94.3 to 99.9%, respectively. The good diagnostic performance of RBT combined with its simplicity, quickness, and affordability makes RBT an ideal (or close to) stand-alone point-of-care test for early clinical diagnosis and management of human brucellosis and nonmalarial fevers in small and understaffed health facilities and laboratories in endemic areas in Africa and elsewhere.

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“…However, care should be taken not to vaccinate pregnant animals. 5) To ensure a sound population of Holstein cows within the HPI scheme, this ELISA test should be repeated annually since it is a more sensitive test compared to the Rose Bengal, standard agglutination and Coombs test in the diagnosis of brucellosis (Saz et al 1987). 6) Artificial insemination with healthy semen should be imperative for the Heifer International scheme to avoid contamination of these valuable animals from infected bulls.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon

Bayemi

Webb

Nsongka

et al. 2008

Trop Anim Health Prod

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Holstein cattle of a small scale dairy production systems were screened for Brucella abortus antibodies in 21 villages in Cameroon by ELISA. Results show a general seroprevalence of 8.4% in Holstein cattle. Of the 192 cows tested, 14 were infected giving a within-sex seroprevalence of 7.3% while 6/74 bulls were infected with a seroprevalence of 8%. There was no evidence (P=0.11) of differences in seroprevalence between age groups although animals above one year and below three years accounted for nearly half of the infected animals. 64% of infected animals were found in three locations (P=0.015): Kutaba (32%), Bamendankwe (16%) and Finge (16%). A specific control programme should be organized at these locations. Measures should be taken to ensure the eradication of the disease within the population and sound control measures adopted to avoid a further spread of the disease to larger cattle populations. Infected animals should be slaughtered systematically. All farmers should be advised to boil milk before consumption. Vaccination against Brucella abortus should be instituted and use of artificial insemination propagated. In order to ensure a productive and healthy population of Holstein cows within the dairy production scheme, regular Brucella testing should be instituted.Abbreviations OIE World organization for animal health -ELISA enzyme-linked immunosorbent assay

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Enzyme-linked immunosorbent assay for diagnosis of brucellosis

Cited by 22 publications

References 11 publications

Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

Utility of the Rose Bengal Test as a Point-of-Care Test for Human Brucellosis in Endemic African Settings: A Systematic Review

Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon

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