Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes

Fujinami, Aya; Obayashi, Hiroshi; Ohta, Kiyoe; Ichimura, Toshiko; Nishimura, Masataka; Matsui, Hidehito; Kawahara, Yuji; Yamazaki, Masahiro; Ogata, Masakazu; Hasegawa, Goji; Nakamura, Naoto; Yoshikawa, Toshikazu; Nakano, Koji; Ohta, Mitsuhiro

doi:10.1016/j.cccn.2003.09.009

Cited by 144 publications

(106 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, the physiological role of resistin on obesity and insulin resistance is unclear, with some studies reporting a significant association between resistin levels, obesity, and insulin resistance (Fujinami et al 2004), whereas others do not (Lee et al 2003). …”

Section: Discussionmentioning

confidence: 99%

The effects of acute exercise on serum adiponectin and resistin levels and their relation to insulin sensitivity in overweight males

et al. 2006

View full text Add to dashboard Cite

The purpose of this study was to investigate the effects of a submaximal aerobic exercise bout on adiponectin and resistin levels as well as insulin sensitivity, until 48 h post-exercise in healthy overweight males. Nine subjects performed an exercise bout at an intensity corresponding to approximately 65% of their maximal oxygen consumption for 45 min. Adiponectin, resistin, cortisol, insulin, glucose and insulin sensitivity were measured prior to exercise, immediately after exercise as well as 24 and 48 h after exercise. Data were analyzed using repeated measures ANOVA while Pearson's correlations were performed to identify possible relationship among the assessed variables. There were no significant differences for adiponectin (lg ml À1 ) [pre Insulin sensitivity increased and insulin concentration decreased significantly only immediately after exercise. Furthermore, no significant correlations were observed among the variables assessed except for the expected between insulin level and insulin sensitivity. These results indicate that a submaximal aerobic workout does not result in significant changes in adiponectin and resistin up to 48 h post-exercise. Furthermore, it appears that adiponectin or resistin is not associated with insulin sensitivity.

show abstract

Section: Discussionmentioning

confidence: 99%

The effects of acute exercise on serum adiponectin and resistin levels and their relation to insulin sensitivity in overweight males

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Monocytes and macrophages are thus thought to be the main source of circulating resistin in humans. Human resistin is also considered to be a biomarker or mediator of metabolic and inflammatory diseases, with increased resistin levels having been associated with metabolic disorders such as obesity, insulin resistance, type 2 diabetes and atherosclerotic cardiovascular disease [4][5][6][7][8]. Recent prospective studies such as the Framingham Offspring Study and Multi-Ethnic Study of Atherosclerosis (MESA) thus found that the circulating resistin level was independently associated with the incidence of atherosclerosis [9,10].…”

Section: Introductionmentioning

confidence: 99%

Epigenome-wide association study suggests that SNPs in the promoter region of RETN influence plasma resistin level via effects on DNA methylation at neighbouring sites

et al. 2015

View full text Add to dashboard Cite

Aims/hypothesis To investigate epigenetic regulation of the plasma concentration of resistin, we performed an epigenomewide association study for this variable and DNA methylation (DNAm) in an elderly Japanese cohort and then assessed the relation of single nucleotide polymorphisms (SNPs) associated with the plasma resistin concentration to DNAm level at identified sites. Methods The association of plasma resistin level with DNAm status was examined in 191 nondiabetic elderly men with the Illumina Infinium HumanMethylation450 BeadChip array. The association between DNAm status at specific sites in the flanking region of the resistin gene (RETN) and RETN mRNA abundance was then evaluated with a public data set for 1202 monocyte samples from a multi-ethnic cohort. Finally, the association of DNAm status and SNPs in the promoter region of RETN was assessed in two cohorts comprising a total of 478 Japanese individuals.Results DNAm status at cg02346997 located in the RETN promoter region showed a negative genome-wide significant association with the plasma resistin level (p=6.02×10 −10 ). Four DNAm sites in the RETN promoter region including cg02346997 (p=4.23×10 −70 ) showed a negative genome-wide significant association with RETN mRNA abundance in monocytes. Furthermore, the number of minor alleles of the RETN promoter SNPs rs34861192 and rs3219175 was negatively associated with DNAm level at cg02346997 (p=4.43×10 −17 ). Conclusions/interpretation Our results suggest that RETN promoter SNPs might influence the circulating resistin level through an effect on DNAm at cg02346997 and on RETN mRNA abundance in monocytes.

show abstract

“…Several theories have also evolved to explain the molecular pathway by which obesity leads to type 2 DM. For example, the medical literature is replete with the evidence-based role of serum resistin levels in this nexus between obesity and type 2 DM [41][42][43][44][45]. Increased obesity has been found to elevate serum resistin levels [41,42], which in turn directly correlate with insulin resistance [43].…”

Section: Obesity and Type 2 Diabetes Mellitus: The Nexusmentioning

confidence: 99%

Childhood Diabetes Mellitus and the Double Burden of Malnutrition: An Emerging Public Health Challenge in Developing Countries

Uwaezuoke¹

2015

J Diabetes Metab

View full text Add to dashboard Cite

The 'double burden of malnutrition' refers to the simultaneous existence of both undernutrition and overnutrition in the same population across the life course. Diabetes mellitus (DM) -a major non-communicable disease -has been linked to both chronic undernutrition and obesity; the latter is now assuming prominence in developing countries. The present review aims to highlight the global burden of this non-communicable disease in children and its relationship with the 'double burden of malnutrition'. Type 2 DM has rapidly evolved from a disease of the Western world to a global disease; from a disease of affluence to a disease that now afflicts the poor; and from an adult-onset disease to a disease that is gaining prominence in the pediatric population. Estimates from the International Diabetes Federation show that diabetes affects at least 285 million people worldwide out of which two-thirds occur in developing (low-to middle-income) countries. The global epicenter of diabetes epidemic is located in China and India as Asia has undergone rapid economic development, urbanization and transitions in nutritional status. Overweight and obesity are propelling the worldwide diabetes epidemic. Central obesity is correlated with both insulin resistance and type 2 DM. Moreover, the scourge of chronic undernutrition among children in tropical developing countries may predispose them to malnutrition-modulated diabetes mellitus (MMDM) later in life. Children in these regions are thus faced with a health-related dilemma. There is an urgent need to develop a comprehensive prevention program targeted at reducing diabetes risk and promoting proper nutrition among children in these countries.

show abstract

Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes

Cited by 144 publications

References 19 publications

The effects of acute exercise on serum adiponectin and resistin levels and their relation to insulin sensitivity in overweight males

The effects of acute exercise on serum adiponectin and resistin levels and their relation to insulin sensitivity in overweight males

Epigenome-wide association study suggests that SNPs in the promoter region of RETN influence plasma resistin level via effects on DNA methylation at neighbouring sites

Childhood Diabetes Mellitus and the Double Burden of Malnutrition: An Emerging Public Health Challenge in Developing Countries

Contact Info

Product

Resources

About