Enzyme‐linked immunosorbent assay (ELISA) for IgG antibodies in farmers' lung disease

Bamdad, S.

doi:10.1111/j.1365-2222.1980.tb02093.x

Cited by 23 publications

(6 citation statements)

References 21 publications

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“…Plates were coated with egg white extract (50 /ig/ml). Sera from Group B were examined in duplicate using test conditions described previously for detecting IgG antibodies in farmers' lung disease (Bamdad, 1980),…”

Section: Elisa For Egg-white-specific Iggmentioning

confidence: 99%

“…The enzyme-linked immunosorbent assay (ELISA) (Engvall & Perlmann, 1972) has been shown to be sensitive, specific and reliable assay for IgG antibodies to a variety of antigens, including viral and parasitic antigens (Voller, Bidwell & Bartlet, 1976). Micropolyspora faeni antigens in former lung disease (Bamdad, 1980) and to protein and polysaccharide antigens of Aspergillus fumigatus (Sepulveda, Longbottom & Pepys, 1979). It has also been used to detect total IgE (Hoffman, 1973) and specific IgE antibodies to some inhalant allergens (Eriksson & Ahlstedt, 1977).…”

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confidence: 99%

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IgE antibodies to food allergens detected by ELISA, RAST and monkey PCA

Bamdad

Goodwin

Hill

1983

Clin Experimental Allergy

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Section: Elisa For Egg-white-specific Iggmentioning

confidence: 99%

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confidence: 99%

IgE antibodies to food allergens detected by ELISA, RAST and monkey PCA

Bamdad

Goodwin

Hill

1983

Clin Experimental Allergy

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“…The comrnonest responsible aetinomyeetes are Micropolvspora facni (M, facni) and Thermoactinomyces vulgaris. Several methods are available for making a serologieal diagnosis of this disease: enzyme-linkedimmuno-sorbent assay (ELISA) [1,2]. Radioimmunoassay (RIA) [3], latex agglutination test [4,5].…”

mentioning

confidence: 99%

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Gari

Pinon

Poirriez

et al. 1986

Clin Experimental Allergy

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Abstract. Until now the detection of chymotrypsic or polysaccharidic arcs among precipitating systems indicated a positive diagnosis of Farmer's lung disease (FLD). It was shown that the occurrence of chymotrypsic activity is not absolutely correlated with the presence of clinical disease. The presence of different classes of specific precipitating antibodies was looked for and correlated with clinical symptoms in an attempt to find additional positive criteria for the diagnosis of FLD. The enzyme‐linked immunofiltration assay (ELIFA) was adapted to detect the different specific antibody classes. 1400 serum samples were studied from 1350 farmers exposed to mouldy hay. Of 1358 samples examined from asymptomatic subjects tested as part of a systematic screening of the profession. 1356 had either no precipitins or have precipitins detected solely against non‐chymotrypsic and non‐polysaccharidic antigens. Ten specimens with precipitins joined the study. Only two serum samples from two symptomless individuals showed positive serological criteria for FLD as evidenced by the presence of a chymotrypsic band. The remaining forty‐two serum samples from twenty‐four patients with clinical signs of extrinsic allergic alveolitis had serological criteria for FLD as either chymotrypsic or polysaccharidic arcs. Only specific IgG in asymptomatic subjects was demonstrated, and when a supplementary class of specific antibody (IgM, IgA or IgE) was detected, there was clinical evidence of FLD in all except one case. Thus ELIFA could prove to be a useful tool for the positive diagnosis and prognosis of patients with FLD. In addition this method allows the recognition of a particular functional antigenic fraction of M. faeni by all classes of specific antibodies between different serum samples.

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“…The IgA antibody showed fewer bands (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). But all sera showed an IgA response directed to at least one of the group of bands at 54-to 70-kD.…”

Section: Resultsmentioning

confidence: 99%

“…A double dialy sis method for the production of extracellular growth products of M. faeni was introduced some years ago [131 and was found to be superior to other methods in use at that time. Since then, Edwards' double dialysis has been used to produce antigens of the other organisms, T vulga ris, T. candidus and Saccharomonospora viridis [14][15][16] as sociated with farmer's lung.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Methods of Production of Farmer’s Lung Antigens

Melinn

McLaughlin²

1992

Int Arch Allergy Immunol

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The extracellular growth products of Micropolyspora faeni, Thermoactinomyces vulgaris and Thermoactinomyces candidus were prepared by three methods; (1) double dialysis; (2) growth in tryptone soya broth (TSB) and (3) synthetic broth (AOAC). It was found that double dialysis was superior to growth in TSB or AOAC for the production of M. faeni antigens, as determined by immunoblot. Double dialysis, TSB and AOAC were similar for antigens of T. vulgaris and T. candidus. Both organisms showed bands of the same mobility on immunoblot and also produced lines of identity on double diffusion.

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Enzyme‐linked immunosorbent assay (ELISA) for IgG antibodies in farmers' lung disease

Cited by 23 publications

References 21 publications

IgE antibodies to food allergens detected by ELISA, RAST and monkey PCA

IgE antibodies to food allergens detected by ELISA, RAST and monkey PCA

Enzyme‐linked immunofiltration assay (ELIFA) for the detection of specific antibodies (IgG‐IgM‐IgA‐IgE) in farmer's lung disease

Comparison of Methods of Production of Farmer’s Lung Antigens

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