Certain populations of lymphocytes have been shown to reduce tetrazolium salts, indicating that superoxide anion may be present in lymphocytes. These experiments were done to determine if nitroblue tetrazolium reduction by lymphocytes was due to the presence of superoxide anion. Mitogen-activated lymphocytes showed increased nitroblue tetrazolium reduction compared to unstimulated cells, and the cells reducing nitroblue tetrazolium were both T-cells and non-T-cells. Release of superoxide anion or hydrogen peroxide by either resting or stimulated lymphocytes was not detected. There was no difference between resting and stimulated lymphocytes in the amount of chemiluminescence produced in the presence of lucigenin, an agent which appears to be sensitive to intracellular and extracellular superoxide anion. The results, then, indicate superoxide anion is not present in lymphocytes.
The extracellular growth products of Micropolyspora faeni, Thermoactinomyces vulgaris and Thermoactinomyces candidus were prepared by three methods; (1) double dialysis; (2) growth in tryptone soya broth (TSB) and (3) synthetic broth (AOAC). It was found that double dialysis was superior to growth in TSB or AOAC for the production of M. faeni antigens, as determined by immunoblot. Double dialysis, TSB and AOAC were similar for antigens of T. vulgaris and T. candidus. Both organisms showed bands of the same mobility on immunoblot and also produced lines of identity on double diffusion.
The incidence of detection of antibodies against antigens derived from two thermophilic actinomycetes in patients clinically suspected of having Farmer's Lung was assessed. Approximately 25% of samples submitted over a three year period were found to be positive in either the ELISA test or the CIEP test for antigens derived from M. faeni and T. vulgaris. The ELISA test proved to be more sensitive than the CIEP test in the detection of antibodies directed against both organisms.
We have studied normal human lymphocytes stimulated by concanavalin A to determine the rate and extent of development of cytoplasmic granules. Morphological studies on stimulated lymphocytes were undertaken at the light and electron microscopic level. The percentage of T lymphocytes bearing different CD antigens (CD3, CD4, and CD8) that also contained cytoplasmic granules was also assessed. Granules were identified in a high percentage of lymphocytes bearing each of the three CD markers studied.
A patient with both chronic lymphatic leukemia and multiple myeloma is presented. Characterisation of the leukemic cells showed them to possess Fc receptors but to lack SmlG and C3 receptors. Intracellular immunoglobulin was not detected within the leukemic cells. The multiple myeloma presented as a soft tissue mass on the chest wall and produced an IgGK paraprotein. The possible relationship of the two disorders is discussed.
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