Enzyme-linked immunosorbent assay (ELISA) for direct quantification of surface-bound platelet immunoglobulins

Hansen, Ole Paaske; Overballe, C.; Hippe, Erik

doi:10.1080/00365518309168438

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…Both the immunoglobulins in the alpha granules and the surfacebound Ig are reported in the literature [2,4,6,7,9,11]. However antibodies against the glycoprotein IIb/IIIa in patients with IMF, as in our patient, have not previously been reported in the literature.…”

Section: Discussionmentioning

confidence: 40%

Myelofibrosis and idiopathic thrombocytopenic purpura

Seelen

Meijer

Posthuma

et al. 1997

Annals of Hematology

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Section: Discussionmentioning

confidence: 40%

Myelofibrosis and idiopathic thrombocytopenic purpura

Seelen

Meijer

Posthuma

et al. 1997

Annals of Hematology

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“…These preliminary results confirm the results of Cines & Schreiber (1979) who found that serum platelet antibody levels are less reliable in assessing the disease activity than the direct platelet IgG determination. The frequency of platelet-bound IgG was quite similar to that found by Paaske Hansen et al (1983) using an enzyme-linked immuno-assay.…”

Section: Discussionmentioning

confidence: 99%

Microplate enzyme immuno‐assay for detection of platelet antibodies

Taaning

1985

Tissue Antigens

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A simplified and sensitive enzyme immuno‐assay employing microtiter plates as the solid phase carrier for detection of circulating platelet antibodies and bound antiplatelet IgG has been described. The assay was done using fresh as well as frozen platelets and commercially available peroxidase labeled anti‐human IgG. The sensitivity of the enzyme immuno‐assay was found similar or superior to that of the platelet suspension immunofluorescence test and superior to the lymphocyte cytotoxicity test and the platelet complement fixation test. The use of platelets frozen in the wells of the microtiter plates (stored for up to 17 months at —20°C without loss of antigenicity) facilitates the performance of the test. In addition the small volumes of sera and platelets needed in using microtiter plates makes the assay particularly suitable in testing platelets from thrombocytopenic patients. In one of 31 sera from normal Zwa‐negative donors a weak anti‐Zwa (PIA1) was found only detectable by enzyme immuno‐assay. Alloantibodies were found in 14 of 23 patients suffering from non‐haemolytic transfusion reactions. All of three patients with post‐transfusion purpura had anti‐Zwa antibodies in serum. Anti‐Zwa antibodies were demonstrated in the sera from all of 9 Zwa‐negative mothers, who had given birth to children with alloimmune neonatal thrombocytopenia. In 8 of 12 Zwa‐positive mothers alloantibodies of other specificities were found in the serum. In one case the antibody was a known anti‐Baka. Six of 7 patients with autoimmune thrombocytopenic purpura had values of platelet‐bound IgG exceeding the normal range and circulating platelet antibodies were seen in 4 patients.

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Demonstration of human autoantibodies by quantitative enzyme immunoassays

Gripenberg¹,

Kurki²

1986

Journal of Immunological Methods

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Enzyme-linked immunosorbent assay (ELISA) for direct quantification of surface-bound platelet immunoglobulins

Cited by 3 publications

References 21 publications

Myelofibrosis and idiopathic thrombocytopenic purpura

Myelofibrosis and idiopathic thrombocytopenic purpura

Microplate enzyme immuno‐assay for detection of platelet antibodies

Demonstration of human autoantibodies by quantitative enzyme immunoassays

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