Demonstration of human autoantibodies by quantitative enzyme immunoassays

Gripenberg, Marianne; Kurki, Pekka

doi:10.1016/0022-1759(86)90160-2

Cited by 17 publications

(3 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is generally accepted for ELISA format assays that antigen should be coated to excess on the microtiter plate [15]. Results presented here indicate that different optimal coating concentrations were obtained for the one-step and two-step bridging assay formats.…”

Section: Discussionmentioning

confidence: 79%

Effect of double antigen bridging immunoassay format on antigen coating concentration dependence and implications for designing immunogenicity assays for monoclonal antibodies

Bourdage

Lee

Taylor

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 79%

Effect of double antigen bridging immunoassay format on antigen coating concentration dependence and implications for designing immunogenicity assays for monoclonal antibodies

Bourdage

Lee

Taylor

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

“…Few reports, concerning mainly SLE, appear in the literature [20]. Although associations with arthralgia, myalgia and cardiac lesions have been reported in autoimmune and cardiac diseases [21,22], the origin and clinical significance of these Abs in LE remain unclear. Interestingly, these Abs were detected also in DLE patients without systemic manifestations and low prevalence of other Abs (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Autoantibody profile in cutaneous lupus erythematosus subsets: correlations between various autoantibodies of the same isotype

Kroumpouzos

Tosca

Konstadoulakis

et al. 1994

Acad Dermatol Venereol

View full text Add to dashboard Cite

The objective of the present study was an extensive analysis of the autoantibody profile in the most eommon subsets of cutaneous lupus erythematosus (LE), acute and subacute cutaneous lupus erythematosus (ACLE, SCLE) as well as discoid lupus, localized or disseminatus (DLE loc, DLE dis). Antibodies (Abs) against glomerular extracts, actin, myosin and cardiolipin were examined in the various cutaneous lupus subgroups, in addition to ANA and Abs against DNA, ENA and IgG (rheumatoid factor). Abs against glomerular extracts were detected mainly in ACLE and SCLE, whereas Abs against actin, myosin and cardiolipin were found in all cutaneous lupus subsets. The detailed record of the autoantibody profile helped in the serological differential diagnosis of the various cutaneous lupus subsets, suggesting also that SCLE and DLE dis are distinct subsets in the spectrum of cutaneous LE. Finally, correlations between autoantibodies of the same isotype were carried out, providing implieations for regulation of autoantibody production.

show abstract

“…) say. Purified nuclei from goat liver were immobilized on polystyrene plates to detect antinuclear antibodies (ANA) 1 ) in patients with systemic lupus erythematosus. The effects of the nuclei concentration, storage of nuclei below 0 °C, and the length of the incubation period was investigated on the assay.…”

Section: Introductionmentioning

confidence: 99%

Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen

Ara¹,

Ali²

1993

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Summary: An enzyme-linked immunosorbent assay to detect antinuclear antibodies in the sera of patients with autoimmune diseases is described. Goat liver nuclei were immobilized on polystyrene plates and antinuclear antibodies were used to standardize the assay. The effects of variables, such as the nuclei concentration, conditions of nuclei storage, and the length of the incubation period were investigated on the assay. Prototype sera with known antibody specificity were used to evaluate the assay. The method described is highly sensitive, autoantibodies being detectable at serum dilutions of 1 : 1000 or higher. According to the intra-and inter-assay coefficient of variation, the results were highly reproducible.

show abstract

Demonstration of human autoantibodies by quantitative enzyme immunoassays

Cited by 17 publications

References 135 publications

Effect of double antigen bridging immunoassay format on antigen coating concentration dependence and implications for designing immunogenicity assays for monoclonal antibodies

Effect of double antigen bridging immunoassay format on antigen coating concentration dependence and implications for designing immunogenicity assays for monoclonal antibodies

Autoantibody profile in cutaneous lupus erythematosus subsets: correlations between various autoantibodies of the same isotype

Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen

Contact Info

Product

Resources

About