Enzyme-linked immunomagnetic electrochemical detection of Salmonella typhimurium

Gehring, Andrew G.; Crawford, Cynda; Mazenko, Ralph S.; Houten, Lisa J. Van; Brewster, Jeffrey D.

doi:10.1016/0022-1759(96)00076-2

Cited by 121 publications

(59 citation statements)

References 27 publications

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“…Doseresponse curves were created for spiked buffer samples to assess the MAAB's overall performance and to provide a basis for comparison with other so-called rapid methods of detection. The LODs determined here for both 15-min and 1-h protocols (ϳ10 4 CFU/ml) compare favorably with those for several other antibody-based systems (LODs in the range of mid-10 3 to 10 5 CFU/ml), including piezoelectric sensors (4,33,43,45,59), fiber optic sensors (44,71,72), an interferometer (57), surface plasmon resonance-based sensors (9,56), and an electrochemical detector (22). ORIGEN, a system based on electrochemiluminescence, has proven to be the most sensitive rapid detection system, with a detection limit of 1,000 CFU/ml (70).…”

Section: Discussionsupporting

confidence: 63%

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 ؋ 10 4 CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross-reactivity tests were performed with Escherichia coli and Campylobacter jejuni. To determine whether the MAAB has potential as a screening tool for the diagnosis of asymptomatic Salmonella infection of poultry, chicken excretal samples from a private, noncommercial farm and from university poultry facilities were tested. While the private farm excreta gave rise to signals significantly above the buffer blanks, none of the university samples tested positive for S. enterica serovar Typhimurium without spiking; dose-response curves of spiked excretal samples from university-raised poultry gave limits of detection of 8 ؋ 10 3 CFU/g.

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Section: Discussionsupporting

confidence: 63%

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Taitt

Shubin

Angel

et al. 2004

Appl Environ Microbiol

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“…The electrochemical techniques usually employed for immunoassays are mostly based on amperometric methods [16][17][18][19] and voltammetric methods. [20][21][22] For immunosensors based on electrochemical methods, it is also possible to miniaturize the electrode for field applications. 19 Wide varieties of electrodes such as the carbon paste electrode (CPE), 17,18,25,26 glassy carbon electrode, 20 platinum electrode 27 and gold electrode, 28 have been used for electrochemical immunosensors.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for the Detection of Vibrio cholerae O1 Using Disposable Screen-printed Electrodes

et al. 2006

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A disposable amperometric immunosensor was studied for the rapid detection of Vibrio cholerae (V. cholerae), the causative agent of cholera, employing an indirect sandwich enzyme linked immunosorbent assay (ELISA) principle. Screen-printed electrodes (SPEs) were fabricated (by using commercial and homemade carbon inks), electrochemically characterized and the assay conditions were optimized for capturing antibodies and antigen. Whole cell lysate (WCL) of V. cholerae was used to raise antibodies in rabbits and mice. The antibodies raised against WCL of V. cholerae were found to be specific, and no cross reactivity was observed with other enteric bacteria. 1-Naphthyl phosphate was used as a substrate with the amperometric detection of its enzymatic hydrolysis product 1-naphthol at a potential of +400 mV vs. Ag/AgCl reference electrode. A comparison between the amperometric detection technique and the standard ELISA was made in terms of the total assay time, the amount of biological materials used and the sensitivity of detection. The minimum detection limit of the amperometric immunosensor for V. cholerae was found to be 10 5 cells/ml in 55 min, while ELISA detected 10 6 cells/ml in 4 h.

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“…or specific serotypes in a variety of food (3,8,13,18,21,25). Other authors developed immunoelectrochemical assays or piezoelectric flow injection analysis (FIA) biosensors to detect Salmonella enterica serotype Typhimurium (2,7,26). However, although these biosensors have the potential to provide direct label-free detection of bacteria, these piezoelectric FIA systems have a low level of sensitivity, and neither has been applied to food samples.…”

mentioning

confidence: 99%

Comparison of PCR, Electrochemical Enzyme-Linked Immunosorbent Assays, and the Standard Culture Method for Detecting Salmonella in Meat Products

Croci

Delibato

Volpe

et al. 2004

Appl Environ Microbiol

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An electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with flow injection analysis (ELISA-FIA) and a PCR-based method using ST11 and ST15 primers for detecting salmonellae in meat were evaluated in comparison with the International Organization for Standardization (ISO) culture method. The methods were applied to experimentally contaminated and naturally contaminated meat samples. The results showed that both ELISA-FIA and PCR allowed detection of salmonella in a product contaminated with a low number of the microorganisms (1 to 10 salmonellae/25 g) after only 5 h of incubation of preenrichment broth, and they were just as effective as the ISO method.The incidence of foodborne infection by Salmonella continues to be an important problem in the United States (16) and in the European Community (4, 16).Raw and undercooked meat, eggs, milk, and especially poultry are the most commonly implicated vehicles of Salmonella spp. infection (10,15,16,20,24). For foods to be considered "salmonella free," European legislation requires that from 1 to 25 g be tested, depending on the type of food. The standard culture method (ISO 6579/2002) for detection of salmonella in food is rather sensitive and quite inexpensive, but it requires 4 to 5 days to generate results.Given that efforts to prevent salmonellosis must be applied at all phases of the food production chain, based on the Hazard Analysis Critical Control Point (HACCP) system (23), the methods for detecting salmonella need to be more rapid than the ISO method. To this end, new technologies have been used to develop rapid methods. Specifically, a number of antibodyantigen-based methods have been developed to detect Salmonella spp. or specific serotypes in a variety of food (3,8,13,18,21,25). Other authors developed immunoelectrochemical assays or piezoelectric flow injection analysis (FIA) biosensors to detect Salmonella enterica serotype Typhimurium (2, 7, 26). However, although these biosensors have the potential to provide direct label-free detection of bacteria, these piezoelectric FIA systems have a low level of sensitivity, and neither has been applied to food samples.Methods based on PCR for the detection of salmonella in food have also been developed (12,17,19).All of the above methods require that a preenrichment step be performed (5,9,14,18,22).The objective of the present study was to evaluate two rapid techniques for detection of salmonella: an electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with FIA (ELISA-FIA) (6) and a PCR method using ST11 and ST15 primers (1).The experiments were first performed with experimentally contaminated samples to reduce and optimize the preenrichment times; the two methods were then applied to naturally contaminated meat samples, and the results were compared to those obtained with the International Organization for Standardization (ISO) method. MATERIALS AND METHODSThe experiments were performed on the following: (i) experimentally contaminated meat samples, to establish the minimum preenrichment...

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Enzyme-linked immunomagnetic electrochemical detection of Salmonella typhimurium

Cited by 121 publications

References 27 publications

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Detection ofSalmonella entericaSerovar Typhimurium by Using a Rapid, Array-Based Immunosensor

Amperometric Immunosensor for the Detection of Vibrio cholerae O1 Using Disposable Screen-printed Electrodes

Comparison of PCR, Electrochemical Enzyme-Linked Immunosorbent Assays, and the Standard Culture Method for Detecting Salmonella in Meat Products

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