Enzyme linked immuno assay of cardiac troponin T for the detection of acute myocardial infarction in patients

Adaptation of the Troponin T ELISA Test to a Microplate Immunoassay Reader

“…In figure 4, typical changes of serum troponin T and enzyme values in a patient with acute myocardial infarction with early reperfiision are presented. This clinical result obtained with our modified method corresponds well to previous observations made with automated assays for troponin T in serum (3,4,7,11,16).…”

“…Initially the cytosolic troponin T, but later also the coniplexed troponin T are released from damaged muscle cells into the circulation (3). This release depends on the early reperfusion of the damaged area, and it can be utilized to follow the progress of a myocardial infarction (3,4). Troponin T is found in both myocardial and in skeletal muscle.…”

The Introduction and Clinical Use of Cardiac‐Specific Troponin Assays

“…The next major breakthrough came in 1989, with the dissemination of immunoassays for cTnT, ushering in the troponin era. 2 The troponin complex of proteins regulates contractile activity by controlling the timing and extent of myosin-actin interactions in response to changes in cytosolic calcium ion concentration. Troponin I and troponin T in the heart are coded by specific genes, resulting in the cardiac-specific isoforms cTnI and cTnT that are distinct from those in skeletal muscle.…”

Biochemical Markers of Myocardial Damage

“…The laboratory of Hugo Katus in Germany focused on developing an immunoassay for the measurement of cardiac troponin T (cTnT) [12] and our research group in Jack Ladenson's laboratory at Washington University concentrated on cardiac troponin I (cTnI) [13]. Phase one clinical studies demonstrated that both cTnI and cTnT will be elevated after a heart attack in patients [13], [14].…”