Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance

Wierschem, Matthias; Walz, Olga; Mitsos, Alexander; Termuehlen, Maren; Specht, Anna Lena; Kissing, Katrin; Skiborowski, Mirko

doi:10.1016/j.cep.2016.11.001

Cited by 13 publications

(23 citation statements)

References 23 publications

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“…This model was extended with physical properties of the used components [20] and kinetic data based on a recent publication [21]. Furthermore, the operating window is described in which the ERD is feasible for experimental investigations and simulation.…”

Section: Resultsmentioning

confidence: 99%

“…This leads to a higher ratio of the substrates inside the reactive section. In a recent publication [21] it was shown that for greater values of around 5 for the substrate ratio χ EtBu/BuOH the reaction rate is decreasing. Thus, a higher specific surface area leads to lower conversions.…”

Section: Sensitivity Analysismentioning

confidence: 99%

“…Kinetic Data: The kinetics of the transesterification of ethyl butyrate was investigated in a recent study [21]. Based on these experimental results we assumed that the catalyzed system is not mass transfer controlled so that the rate-determining step is the kinetic step.…”

Section: Model Descriptionmentioning

confidence: 99%

“…Note that, the mass per meter is not an indicator for the actual amount of enzyme inside or on the packing, since the coating mainly consists of solgel. The activity of the immobilizates was compared in a recent study [21]. To prevent the enzyme beads from repetitive shrinkage and swelling and therefore breakage, cold overflow was turned on twice a day for approximately 1 h to wet the enzyme beads inside the column.…”

Section: Enzymatic Reactive Distillationmentioning

confidence: 99%

“…The collection of fundamental data including property data [20] and the reaction kinetics [21] was presented recently. In this paper, we address the investigations of packing properties, we identify the operating window and we develop a mathematical model, followed by experiments which were carried out in a pilot-scale ERD column and a conventional distillation column.…”

Section: Problem Statementmentioning

confidence: 99%

See 4 more Smart Citations

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

Wierschem

Schlimper

Heils

et al. 2017

Chemical Engineering Journal

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Highlights• Butyl butyrate synthesis by Enzymatic Reactive Distillation is possible• Established rate-based model to describe Enzymatic Reactive Distillation • First time validation for an Enzymatic Reactive Distillation process model AbstractFor enzyme-catalyzed reactions, batch processes using stirred tank reactors are the state-ofthe-art production mode. The yield of the process may be limited by reaction equilibrium, product inhibition of the enzyme, low concentrations and possibly low reaction rates, while the recovery of the product may be limited due to thermodynamic constraints such as azeotropes. Using enzymes in an integrated reactive distillation process can overcome these limitations and provides a cost advantage over classic batch reactor processes.The aim of this paper is i) to report the successful pilot-scale experimental validation of an Enzymatic Reactive Distillation (ERD) process for the synthesis of butyl butyrate and ii) to Pilot-Scale Validation of Enzymatic Reactive Distillation for Butyl Butyrate ProductionWierschem et al.2 establish a rate-based model for conceptual process design which can be quickly adapted to other systems.The main novelty is the application of a continuous RD column with enzymes as a heterogeneous catalyst provided in two different types of catalytic packing: loosely filled immobilized enzyme beads in standard packings with catalyst pockets and gauze packings with catalytic coating.Experimental pilot-scale experiments show the feasibility of ERD and allow the comparison of the different packing types based on catalytic performance as well as stability.Furthermore, these experiments are used to validate a predictive rate-based model to describe ERD which can be used to check the sensitivity of process and design parameters as well as to provide a quick adaption to other systems for quick evaluation.

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Section: Resultsmentioning

confidence: 99%

Section: Sensitivity Analysismentioning

confidence: 99%

Section: Model Descriptionmentioning

confidence: 99%

Section: Enzymatic Reactive Distillationmentioning

confidence: 99%

Section: Problem Statementmentioning

confidence: 99%

See 3 more Smart Citations

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

Wierschem

Schlimper

Heils

et al. 2017

Chemical Engineering Journal

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Improvement of adhesion properties of enzyme‐loaded coating on random packing in transesterification

Wang

Liang

et al. 2020

Asia-Pacific J Chem Eng

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The stability of immobilized enzyme on packing is an important factor to limit the development of enzyme-catalyzed reaction distillation. The modified sol loaded with Candida antarctica lipase B (CALB) was fixed on random packing (316 L stainless steel wire mesh) by spraying method to form an enzymeloaded coating, which was applied to catalyze the esterification of ethyl acetate and n-butanol. The addition of 1-[3-(trimethoxysilyl)propyl]ureido (UPS) enhanced the stability of the CALB, and the mechanism of its combination was explored from a microscopic perspective. And then, it was confirmed by scratch resistance test that adhesion of enzyme-loaded coating in packing was improved 19%. The esterification reaction showed that the addition of UPS had little effect on the conversion rate while improving the stability of the coating.These results suggest that the effect of UPS treatment on the enzyme-loaded coating performance significantly enhanced the long-term effect of enzymeloaded packing.

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Reactive Separations for In Situ Product Removal of Enzymatic Reactions: A Review

Fellechner

Blatkiewicz

Смирнова

2019

Chemie Ingenieur Technik

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The topic of integrating enzymatic reactions with in situ product removal is addressed. Different integrated reactive separations structured accordingly to the corresponding unit operations, i.e., reactive distillation, reactive chromatography, reactive crystallization, and extractive biocatalysis, are discussed. Special attention is given to their realization with homogenous and heterogeneous biocatalysis. Various enzyme immobilization techniques are presented and distinct strategies for installation of heterogeneous catalysts into process equipment are discussed.

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Enzyme kinetics for the transesterification of ethyl butyrate with enzyme beads, coated packing and ultrasound assistance

Cited by 13 publications

References 23 publications

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

Improvement of adhesion properties of enzyme‐loaded coating on random packing in transesterification

Reactive Separations for In Situ Product Removal of Enzymatic Reactions: A Review

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