Enzyme‐Initiated Free‐Radical Polymerization of Molecularly Imprinted Polymer Nanogels on a Solid Phase with an Immobilized Radical Source

Attieh, Mira Daoud; Zhao, Youliang; Elkak, Assem; Falcimaigne-Cordin, A.; Haupt, Karsten

doi:10.1002/anie.201612667

Cited by 41 publications

(15 citation statements)

References 29 publications

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“…Some ten percent of MIP papers describe artificial receptors for proteins [7,[10][11][12][13], including enzymes [13][14][15][16][17][18][19][20][21]. Molecularly imprinted polymers have been mostly developed for binding of targets, thus mimicking the function of antibodies.…”

Section: Preparation Of Surface Imprinted Mipsmentioning

confidence: 99%

“…Since then, the technique of self-polymerization has been employed in several studies for the imprinting of proteins on various platforms such as magnetic [51,52], gold [53], or silica nanoparticles [54,55], silicon nanowires [56], 4-vinylphenylboronic acid-based monolithic skeletons [57], gold electrodes [58,59], or multi-walled carbon nanotubes [60]. These studies showed that apart from the advantage of controllable thickness, MIPs comprised of polydopamine exhibit good to excellent binding properties, have high hydrophilicity, biocompatibility, as well as pH (3)(4)(5)(6)(7)(8)(9)(10)(11) and longtime stability [61].…”

Section: Electropolymerizationmentioning

confidence: 99%

“…Very recently, HRP-initiated radical polymerization has been introduced by Haupt's group as a tool for the preparation of MIPs for small molecules such as 2,4-dichlorophenoxyacetic acid (2,4-D) and salicylic acid, but also for the protein trypsin [10]. HRP with H 2 O 2 as the substrate and a mediator molecule such as acetylacetone generates radicals that can initiate the polymerization of vinyl and acrylate monomers (see Scheme 2).…”

Section: Enzyme-initiated Mip Synthesismentioning

confidence: 99%

“…For trypsin, the particles had a size of 25 nm and an IF of 4. Regarding their recognition properties, enzyme-initiated MIP nanoparticles were shown to be comparable to or even outperforming their photo-initiated counterparts [10]. …”

Section: Enzyme-initiated Mip Synthesismentioning

confidence: 99%

“…In fact, the choice of regeneration conditions is a trade-off between complete removal of the target and preservation of the integrity of the binding sites. Methods applied include the application of chaotropic agents, extraction by organic solvents, the use of highly acidic or basic solutions and/or surfactants such as sodium dodecylsulfate (SDS) or Tween 20, sometimes at elevated temperatures [7], and electroelution [10,13,31].…”

Section: Template Removal By Enzymesmentioning

confidence: 99%

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Enzymes as Tools in MIP-Sensors

et al. 2017

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Abstract:Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as "tracers" for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.

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Section: Preparation Of Surface Imprinted Mipsmentioning

confidence: 99%

Section: Electropolymerizationmentioning

confidence: 99%

Section: Enzyme-initiated Mip Synthesismentioning

confidence: 99%

Section: Enzyme-initiated Mip Synthesismentioning

confidence: 99%

Section: Template Removal By Enzymesmentioning

confidence: 99%

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Enzymes as Tools in MIP-Sensors

et al. 2017

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Oxidoreductase‐Initiated Radical Polymerizations to Design Hydrogels and Micro/Nanogels: Mechanism, Molding, and Applications

Wang

Chen

et al. 2018

Advanced Materials

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Due to their 3D cross-linked networks and tunable physicochemical properties, polymer hydrogels with different sizes are applied widely in tissue engineering, drug-delivery systems, pollution regulation, ionic conducting electrolytes, agricultural drought-resistance, cosmetics, and the food industry. Novel, environmentally friendly, and efficient oxidoreductase-initiated radical polymerizations to design hydrogels and micro/nanogels have gained increasing attention. Herein, the recent advances on the use of novel enzyme-initiated systems for hydrogel polymerization, including the mechanisms, and molding of polymeric and hybrid-polymeric networks are reviewed. Preliminary progress related to interfacial enzymatic polymerization for the generation of hybrid micro/nanogels is introduced as an emerging initiating approach. In addition, certain biological applications in tissue engineering, bioimaging, and therapy are demonstrated step by step. Finally, some perspectives on the safety profile of enzymatic formed hydrogels, new enzymatic systems, and potential theranostic applications are discussed.

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Molecularly Imprinted Nanoparticles for Biomedical Applications

2019

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Molecularly imprinted polymers (MIPs) are synthetic receptors with tailor‐made recognition sites for target molecules. Their high affinity and selectivity, excellent stability, easy preparation, and low cost make them promising substitutes to biological receptors in many applications where molecular recognition is important. In particular, spherical MIP nanoparticles (or nanoMIPs) with diameters typically below 200 nm have drawn great attention because of their high surface‐area‐to‐volume ratio, easy removal of templates, rapid binding kinetics, good dispersion and handling ability, undemanding functionalization and surface modification, and their high compatibility with various nanodevices and in vivo biomedical applications. Recent years have witnessed significant progress made in the preparation of advanced functional nanoMIPs, which has eventually led to the rapid expansion of the MIP applications from the traditional separation and catalysis fields to the burgeoning biomedical areas. Here, a comprehensive overview of key recent advances made in the preparation of nanoMIPs and their important biomedical applications (including immunoassays, drug delivery, bioimaging, and biomimetic nanomedicine) is presented. The pros and cons of each synthetic strategy for nanoMIPs and their biomedical applications are discussed and the present challenges and future perspectives of the biomedical applications of nanoMIPs are also highlighted.

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Enzyme‐Initiated Free‐Radical Polymerization of Molecularly Imprinted Polymer Nanogels on a Solid Phase with an Immobilized Radical Source

Cited by 41 publications

References 29 publications

Enzymes as Tools in MIP-Sensors

Enzymes as Tools in MIP-Sensors

Oxidoreductase‐Initiated Radical Polymerizations to Design Hydrogels and Micro/Nanogels: Mechanism, Molding, and Applications

Molecularly Imprinted Nanoparticles for Biomedical Applications

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