Enzymes as Tools in MIP-Sensors

Yarman, Aysu; Jetzschmann, Katharina J.; Neumann, Bettina; Zhang, Xiaorong; Wollenberger, Ulla; Cordin, Aude; Haupt, Karsten; Scheller, Frieder W.

doi:10.3390/chemosensors5020011

Cited by 16 publications

(9 citation statements)

References 111 publications

(138 reference statements)

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“…It should be noted that, in addition to their high affinity and selectivity for the target analyte 41 , MIPs are characterized by high thermal and chemical stability, low cost and easy preparation A further interesting feature of MIP-based electrochemical sensors is that they even allow detection of non-electroactive analytes, by exploiting the competition for the binding sites in the MIP between the analyte and a redox probe. The probe must have characteristics similar to the analyte as far as molecular size, ionic charge and binding interactions are concerned 42 ; when operating under these experimental conditions, the probe signal will scale inversely with the analyte concentration.…”

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confidence: 99%

Electrochemosensor for Trace Analysis of Perfluorooctanesulfonate in Water Based on a Molecularly Imprinted Poly(o-phenylenediamine) Polymer

et al. 2018

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This work is aimed at developing an electrochemical sensor for the sensitive and selective detection of trace levels of perfluorooctanesulfonate (PFOS) in water. Contamination of waters by perfluorinated alkyl substances (PFAS) is a problem of global concern due to their suspected toxicity and ability to bioaccumulate. PFOS is the perfluorinated compound of major concern, as it has the lowest suggested control concentrations. The sensor reported here is based on a gold electrode modified with a thin coating of a molecularly imprinted polymer (MIP), prepared by anodic electropolymerization of o-phenylenediamine (o-PD) in the presence of PFOS as the template. Activation of the sensor is achieved by template removal with suitable a solvent mixture. Voltammetry, a quartz crystal microbalance, scanning electron microscopy and elemental analysis were used to monitor the electropolymerization process, template removal, and binding of the analyte. Ferrocenecarboxylic acid (FcCOOH) has been exploited as an electrochemical probe able to generate analytically useful voltammetric signals by competing for the binding sites with PFOS, as the latter is not electroactive. The sensor has a low detection limit (0.04 nM), a satisfactory selectivity, and is reproducible and repeatable, giving analytical results in good agreement with those obtained by HPLC-MS/MS analyses.

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confidence: 99%

Electrochemosensor for Trace Analysis of Perfluorooctanesulfonate in Water Based on a Molecularly Imprinted Poly(o-phenylenediamine) Polymer

et al. 2018

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“…15 ). In this case, therefore, there is a “direct conversion” of the analyte into an electrochemically usable signal [ 119 , 197 ].…”

Section: Suitable Electrochemical Signals For Mip-mediated Macromolec...mentioning

confidence: 99%

Electrochemical sensing of macromolecules based on molecularly imprinted polymers: challenges, successful strategies, and opportunities

2022

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Looking at the literature focused on molecularly imprinted polymers (MIPs) for protein, it soon becomes apparent that a remarkable increase in scientific interest and exploration of new applications has been recorded in the last several years, from 42 documents in 2011 to 128 just 10 years later, in 2021 (Scopus, December 2021). Such a rapid threefold increase in the number of works in this field is evidence that the imprinting of macromolecules no longer represents a distant dream of optimistic imprinters, as it was perceived until only a few years ago, but is rapidly becoming an ever more promising and reliable technology, due to the significant achievements in the field. The present critical review aims to summarize some of them, evidencing the aspects that have contributed to the success of the most widely used strategies in the field. At the same time, limitations and drawbacks of less frequently used approaches are critically discussed. Particular focus is given to the use of a MIP for protein in the assembly of electrochemical sensors. Sensor design indeed represents one of the most active application fields of imprinting technology, with electrochemical MIP sensors providing the broadest spectrum of protein analytes among the different sensor configurations. Graphical abstract

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“…However, several parameters have to be carefully selected in order to make good exploitation of this methodology. Indeed, the selection of the adequate elements for the target biorecognition is very important and depends on several criteria such as the polymerization degree, the potential range for the electropolymerization, and the functional groups present in the selected polymer [27,28]. The family of polyaminophenols is advantageous for these effects with a high degree of permselectivity and simple control of the polymer density that can self-limit its growth.…”

Section: Choice Of the Platform Elementsmentioning

confidence: 99%

Novel Electrochemical Molecularly Imprinted Polymer-Based Biosensor for Tau Protein Detection

2021

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A novel electrochemical biosensor based on a molecularly imprinted polymer (MIP) was developed for the impedimetric determination of Tau protein, a biomarker of Alzheimer’s disease (AD). Indeed, a recent correlation between AD symptoms and the presence of Tau proteins in their aggregated form made hyperphosphorylated Tau protein (Tangles) a promising biomarker for Alzheimer’s diagnosis. The MIP was directly assembled on a screen-printed carbon electrode (C-SPE) and prepared by electropolymerization of 3-aminophenol (AMP) in the presence of the protein template (p-Tau-441) using cyclic voltammetry. The p-Tau-441 protein bound to the polymeric backbone was digested by the action of the proteolytic activity of proteinase K in urea and then washed away to create vacant sites. The performances of the corresponding imprinted and non-imprinted electrodes were evaluated by electrochemical impedance spectroscopy. The detection limit of the MIP-based sensors was 0.02 pM in PBS buffer pH 5.6. Good selectivity and good results in serum samples were obtained with the developed platform. The biosensor described in this work is a potential tool for screening Tau protein on-site and an attractive complement to clinically established methodologies methods as it is easy to fabricate, has a short response time and is inexpensive.

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Enzymes as Tools in MIP-Sensors

Cited by 16 publications

References 111 publications

Electrochemosensor for Trace Analysis of Perfluorooctanesulfonate in Water Based on a Molecularly Imprinted Poly(o-phenylenediamine) Polymer

Electrochemosensor for Trace Analysis of Perfluorooctanesulfonate in Water Based on a Molecularly Imprinted Poly(o-phenylenediamine) Polymer

Electrochemical sensing of macromolecules based on molecularly imprinted polymers: challenges, successful strategies, and opportunities

Novel Electrochemical Molecularly Imprinted Polymer-Based Biosensor for Tau Protein Detection

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