Enzyme Inhibition by Allicin, the Molluscicidal Agent ofAllium sativumL. (Garlic)

Singh, Vinay Kumar; Singh, Dinesh

doi:10.1002/(sici)1099-1573(199608)10:5<383::aid-ptr855>3.0.co;2-9

Cited by 30 publications

(18 citation statements)

References 11 publications

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“…Toxicity of nutmeg and mace extracts against L. acuminata is in the range of high molluscicidal activity, the LC 50 value being less than 100 mgL −1 (24,25). Thus, the 96-hour LC 50 of column-purified fraction of nutmeg (3.98 mgL −1 ) and mace (2.77 mgL −1 ) against L. acuminata are lower than those of 96-hour LC 50 values of synthetic molluscicides.…”

Section: Discussionmentioning

confidence: 93%

Molluscicidal Activity of Nutmeg and Mace (Myristica FragransHoutt.) Against the Vector SnailLymnaea Acuminata

Jaiswal

Singh

2009

Journal of Herbs, Spices & Medicinal Plants

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The molluscicidal activity of powdered nutmeg and mace (Myristica fragrans Houtt.) against the vector snail Lymnaea acuminata was evaluated. The toxicity of both the spices was time-and dosedependent. The toxicity of mace powder (96 h LC 50 = 28.61 mgL -1 ) was more pronounced than that of nutmeg powder (96 h LC 50 = 36.95 mgL -1 ). Ethanolic extracts of nutmeg and mace were more toxic than their other organic solvent extracts. The ethanolic extract of mace (24 h LC 50 = 13.33 mgL -1 ) was more effective than the ethanolic extract of nutmeg (24 h LC 50 = 18.04 mgL -1 ) in killing the test animals. The 96 h LC 50 of column purified fraction of mace was 2.77 mgL -1 whereas that of nutmeg was 3.98 mgL -1 . Nutmeg and mace may be used as potent molluscicides.

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Section: Discussionmentioning

confidence: 93%

Molluscicidal Activity of Nutmeg and Mace (Myristica FragransHoutt.) Against the Vector SnailLymnaea Acuminata

Jaiswal

Singh

2009

Journal of Herbs, Spices & Medicinal Plants

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“…However, SDH activity was not altered significantly. Singh and Singh27 have reported that allicin is an uncompetitive inhibitor of AChE and a competitive inhibitor of LDH and alkaline phosphatase in the nervous tissue of L acuminata . It seems that the higher inhibition (as evident from SR values) of AChE, LDH and alkaline phosphatase activity in nervous tissue of snails exposed to allicin + PB or ENT 8184 may be due to the inhibition of certain enzymes which detoxify allicin in the snail.…”

Section: Discussionmentioning

confidence: 99%

Toxicity to the snailLimnaea acuminata of plant-derived molluscicides in combination with synergists

Singh

2000

Pest Manag. Sci.

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The effects of combinations of plant-derived molluscicides with piperonyl butoxide (PB) or ENT 8184 (MGK-264) were studied on different enzyme activities and biogenic amine levels in the nervous tissue of Limnaea acuminata. 24-h in vivo exposure of the nervous tissue of L acuminata to 40% and 80% of the 24-h LC 50 of the plant-derived molluscicides Azadirachta indica oil, Allium sativum powder and oleoresin of Zingiber of®cinale rhizome and their active molluscicidal components azadirachtin, allicin and [6]-gingerol, alone or in combination (1 5) with PB or ENT 8184, caused a signi®cant reduction in the activity of acetylcholinesterase, lactic dehydrogenase, acid and alkaline phosphatases and sodium-potassium ATPase. There was a signi®cant increase in the activity of succinic dehydrogenase. In vivo exposure for 24 h to sub-lethal concentrations of azadirachtin, allicin and [6]-gingerol, singly and with PB or ENT 8184, signi®cantly altered the dopamine and 5-hydroxytryptamine levels in the nervous tissue of L acuminata. It is concluded that these active moieties singly or with PB or ENT 8184 adversely affect all known neurotransmission mechanisms in the snail, either separately or through a complex interaction between the different neurotransmitters.

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“…nerve synapses, so that the post synaptic membrane is in the state of permanent stimulation causing paralysis, ataxia, convulsion and death of animal. 23,24,35 T. indica seed extract caused dose dependent inhibition of phaspholipase A and other enzyme activity of snake venom. 25 Zhanq et al 26 reported the in vitro cytotoxic effect of procynadine on human hepatoma G2 cell.…”

Section: In Vitro Enzyme Inhibitionmentioning

confidence: 97%

Inhibition Kinetics of Acetylcholinesterase and Phosphatases by the Active Constituents of Terminalia arjuna and Tamarindus indica in the Cerebral Ganglion of Lymnaea acuminata

Soni¹,

Singh²,

Singh³

2017

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Introduction: Paper demonstrates effect of the active molluscicidal components arjunolic acid (Terminalia arjuna bark) and procynadine (Tamarindus indica seed) on the activity of acetylcholinesterase (AChE) and phosphatases (ACP/ALP) in the cerebral ganglion of snail Lymnaea acuminata. Materials and Methods: Kinetics of AChE/ACP/ ALP inhibition in the cerebral ganglion of snail Lymnaea acuminata was studied via in vivo (40% and 80% of 96 h LC 50 ) and in vitro treatments (0.3 µg to 7.0 µg) of the column purified fractions, arjunolic acid and procynadine. Results: In vivo exposure of procynadine and arjunolic acid significantly inhibit acetylcholinesterase (AChE), acid phosphatase (ACP) and alkaline phosphatase (ALP) activities in the cerebral ganglion of L. acuminata exposed to 80% of 96h LC 50 . In in vitro treatment maximum inhibition in AChE/ACP/ALP activities in the cerebral ganglion of snail were noted when exposed to 7.0 µg of arjunolic acid and 0.9 µg of procynadine. Column purified fraction of T. arjuna bark and T. indica seed caused non-competitive and uncompetitive inhibition of AChE activity, respectively. Column purified fraction and arjunolic acid of T. arjuna bark caused uncompetitive inhibition of ACP while column purified fraction and procynadine of T. indica seed caused competitive inhibition. Competitivenon-competitive inhibition of ALP activity in the cerebral ganglion of L. acuminata was observed after treatment of column purified active components of both plants. Conclusions: The molluscicidal activity of T. arjuna bark (arjunolic acid) and T. indica seed (procynadine) against snail L. acuminata is due to the inhibition of AChE/ACP/ ALP. Their inhibition kinetics against AChE/ACP/ALP, were different in cerebral ganglion of snail.

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Enzyme Inhibition by Allicin, the Molluscicidal Agent ofAllium sativumL. (Garlic)

Cited by 30 publications

References 11 publications

Molluscicidal Activity of Nutmeg and Mace (Myristica FragransHoutt.) Against the Vector SnailLymnaea Acuminata

Molluscicidal Activity of Nutmeg and Mace (Myristica FragransHoutt.) Against the Vector SnailLymnaea Acuminata

Toxicity to the snailLimnaea acuminata of plant-derived molluscicides in combination with synergists

Inhibition Kinetics of Acetylcholinesterase and Phosphatases by the Active Constituents of Terminalia arjuna and Tamarindus indica in the Cerebral Ganglion of Lymnaea acuminata

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