Enzyme immunoassays of eicosanoids using acetylcholine esterase as label:  an alternative to radioimmunoassay

Pradelles, Philippe; Grassi, Jacques; Maclouf, Jacques

doi:10.1021/ac00284a003

Cited by 679 publications

(297 citation statements)

References 17 publications

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“…LTE 4 in urine was measured by ACE TM Enzyme Immunoassay Kit (Cayman Chemical, Ann Arbor, MI, USA) a sensitive and specific enzyme immunoassay. This assay is based on the competition between free LTE 4 and a LTE 4 tracer (LTE 4 linked to an acetycholinesterase molecule) for a limited number of LTE 4 -specific rabbit antiserum binding sites [1]. All measurements were done in duplicate and the mean value was calculated.…”

Section: Subjectsmentioning

confidence: 99%

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Severien¹,

Artlich²,

Jonas³

et al. 2000

Eur Respir J

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Measurement of leukotriene E 4 (LTE 4 ) in urine is a noninvasive method for assessing changes in the rate of total body cysteinyl leukotriene production. Eosinophil protein X (EPX) has been used to assess eosinophil activity and monitor inflammation in bronchial asthma. The aim of the study was to look for differences in urinary LTE 4 and EPX concentrations between children with stable atopic asthma and healthy controls and to compare asthmatic children with different disease severity. In addition the relationship was evaluated between urinary LTE 4 amd EPX levels and lung function.LTE 4 was also measured (enzyme immunoassay) together with EPX (radioimmunoassay) in urine and lung function tests were carried out in children with mild asthma (steroid-naive) (n=49), moderate to severe asthma (using inhaled steroids) (n=31) and healthy control subjects (n=28).Urinary leukotriene E 4 (LTE 4 ) was significantly higher in children with asthma than in controls (median [25±75 percentile] 238.5 (126.5±375.7) SD 191.8 versus 189 (51±253.2) SD 131.7 pg . mg -1 creatinine; p=0.021). Urinary EPX was also significantly increased in asthmatic children compared with controls (85.5 [64±131.5] SD 76.2 versus 48.5 [43.2±90] 112.1 mg . mmol -1 creatinine; p=0.006). There were no differences in urinary LTE 4 and EPX between the group of mild and the group of moderate to severe asthmatic children. There were significant associations between the urinary LTE 4 and intrathoracic gas volume (ITGV), residual volume (RV), forced expiratory volume in one second (FEV1), forced expiratory capacity (FVC) and maximum expiratory flow rate at 25% of vital capacity (MEF25).Urinary EPX was only correlated with maximum expiratory flow rate at 75% of vital capacity (MEF75). Thus measurement of urinary LTE 4 may predict the degree of airflow obstruction in asthmatic children. Urinary LTE 4 and EPX are useful markers of airway inflammation and can be helpful in guiding asthma management. There was no correlation between LTE 4 and EPX levels. Eur Respir J 2000; 16: 588±592.

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Section: Subjectsmentioning

confidence: 99%

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Severien¹,

Artlich²,

Jonas³

et al. 2000

Eur Respir J

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“…O exsudato aspirado foi centrifugado (500g/10min) e a celularidade total e diferencial foi determinada, usando câmara de Neubauer e corante Diff-Quick, respectivamente. O sobrenadante desse material aspirado foi coletado e guardado à -20°C para posterior análise da concentração de prostaglandina E2 (PGE2), feita por enzima imunoensaio, usando estojo adquirido comercialmente, como descrito previamente (10) . Nós descrevemos previamente que a permeabilidade vascular na artrite por Zy é máxima às 3h de artrite (9) …”

Section: Methodsunclassified

“…Zy (1mg) foi injetado intra-articularmente (ia) e o IC foi medido na 6 a hora após a indução da artrite no exsudato articular usando câmara de Neubauer. LN (10,30 Figura 4a -Efeito da administração intra-articular de (i.a.) de azul de metileno (AM 500µg/joint) ou hemoglobina (30µmol/junta) sobre a incapacitação articular (IA) na artrite induzida por zymosan (Zy) em ratos.…”

Section: Efeito De Inibidores De Nos No Edema Articular E Naunclassified

Efeito de inibidores da sintase de óxido nítrico na dor inflamatória articular e influxo celular da artrite induzida por zymosan em ratos

Rocha¹,

Rocha²,

Peixoto³

et al. 2003

Rev. Bras. Reumatol.

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“…Analysis of prostanoids used solid-phase ETA; this analytical technique of eicosanoids is equivalent and simpler than standard RIAA (Pradelles et al,, 1985). All these ETA kits may be purchased from Cayman Chemical (Ann Arbor, MI, USA).…”

Section: Analytical Techniquementioning

confidence: 99%

“…A standard curve was obtained by plotting B/B0% (the absorbance measured on the bound fraction with prostanoid divided by the absorbance in the absence of competitor) vs picograms of standard, by well. Fitting the standard curve and calculating the quantity of prostanoid in samples were performed with a nonlinear curve-fitting program (Pradelles et a!., 1985). Sensitivities were, respectively, 0.45, 2, and 3 pg/well for 11-dehydro-TkB2, 6 keto-PGF1 , and LTC4; 11-dehydro-TxB 2 was selected because its reliability is better than that of TxB 2 , as it cannot be released from activated platelets (GranstrOm and Kumlin, 1987); indeed, conversion from glial TxB2 to 11-dyhydro-TxB2 is mostly extraneural, but GrandstrOm and Kumlin (1987) have shown that in some tissue this conversion takes place only when NAD is added.…”

Section: Analytical Techniquementioning

confidence: 99%

The influence of one hyperbaric oxygen-induced seizure on brain eicosanoid content

Mialon¹,

Barthelemy²

1991

Molecular and Chemical Neuropathology

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The levels of prostaglandin F 1 (6-keto-PGF1,j, thromboxane B2 (11-dehydro-TxB2), and peptidoleukotriene C 4 (LTC4) were measured (acetyicholinesterase immunoassay) in the frontal cortex (FC) and the striatum (SA) of the rat brain to study the possible role of eicosartoids in seizures induced by hyperbaric oxygen (HBO). The rats were exposed to (1) hyperbaric oxygen (HBO, 6 ATA 02) up to the first seizure (2) compressed air (6 ATA air, i.e., 1.25 ATA 02) or (3) atmospheric pressure (1 ATA air, i.e., 0.21 ATA 02); there was no seizure in groups 2 and 3. Transition from 6 ATA to atmospheric pressure was obtained in 15 mm; the rats were then decapitated and their heads frozen in liquid nitrogen before extraction and analysis of prostanoids. Whatever the conditions, cortical levels of 6-keto-PGF4c, and 11-dehydro-Tx B2 are higher than striatal levels; considering the same area, 11-dehydro-Tx B2 and LTC 4 concentrations were not significantly different whatever the condition, but there is a trend for lower 6-keto-PGF1 levels in FC after HBO seizure. Biochemical mechanisms are discussed. Eicosanoids do not seem to play a major role in HBO seizures, although some modifications of their metabolism may take place.

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Enzyme immunoassays of eicosanoids using acetylcholine esterase as label: an alternative to radioimmunoassay

Cited by 679 publications

References 17 publications

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Efeito de inibidores da sintase de óxido nítrico na dor inflamatória articular e influxo celular da artrite induzida por zymosan em ratos

The influence of one hyperbaric oxygen-induced seizure on brain eicosanoid content

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