Enzyme Immunoassays for 15-F2t Isoprostane-M, an Urinary Biomarker for Oxidant Stress

Sasaki, D.; Yuan, Ying; Gikas, Katherine; Kanai, Kazuo; Taber, Douglass F.; Morrow, Denise; Roberts, L. Jackson; Callewaert, Denis M.

doi:10.1007/978-1-4615-0193-0_82

Cited by 19 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urine samples were analyzed for 8‐isoPGF levels using a competitive ELISA (OxisResearch, Portland, OR) (Sasaki et al ). Briefly, samples were mixed with an enhancing reagent to eliminating interference due to non‐specific binding.…”

Section: Methodsmentioning

confidence: 99%

A longitudinal study of atrazine and 2,4‐D exposure and oxidative stress markers among iowa corn farmers

Lerro

Freeman

Portengen

et al. 2017

Environ and Mol Mutagen

View full text Add to dashboard Cite

Reactive oxygen species, potentially formed through environmental exposures, can overwhelm an organism’s antioxidant capabilities resulting in oxidative stress. Long-term oxidative stress is linked with chronic diseases. Pesticide exposures have been shown to cause oxidative stress in vivo. We utilized a longitudinal study of corn farmers and non-farming controls in Iowa to examine the impact of exposure to the widely used herbicides atrazine and 2,4-dichlorophenoxyacetic acid (2,4-D) on markers of oxidative stress. 225 urine samples were collected during five agricultural time periods (pre-planting, planting, growing, harvest, off-season) for 30 farmers who applied pesticides occupationally and 10 controls who did not; all were non-smoking men ages 40–60. Atrazine mercapturate (atrazine metabolite), 2,4-D, and oxidative stress markers (malondialdehyde [MDA], 8-hydroxy-2′-deoxyguanosine [8-OHdG], and 8-isoprostaglandin-F2α [8-isoPGF]) were measured in urine. We calculated β estimates and 95% confidence intervals (95%CI) for each pesticide-oxidative stress marker combination using multivariate linear mixed-effect models for repeated measures. Farmers had higher urinary atrazine mercapturate and 2,4-D levels compared to controls. In regression models, after natural log transformation, 2,4-D was associated with elevated levels of 8-OHdG (β=0.066, 95%CI=0.008–0.124) and 8-isoPGF (β=0.088, 95%CI=0.004–0.172). 2,4-D may be associated with oxidative stress because of modest increases in 8-OHdG, a marker of oxidative DNA damage, and 8-isoPGF, a product of lipoprotein peroxidation, with recent 2,4-D exposure. Future studies should investigate the role of 2,4-D-induced oxidative stress in the pathogenesis of human diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

A longitudinal study of atrazine and 2,4‐D exposure and oxidative stress markers among iowa corn farmers

Lerro

Freeman

Portengen

et al. 2017

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…Other interfering contaminants may be released from solid phase cartridges or from plastics used in the assay [12]. Studies comparing the values obtained by immunoassay and GC/MS measurements have shown very different results depending on the antibodies used, the extent of sample purification, and the fluid measured, with some studies finding excellent correlation [10, 13, 14] and other finding either modest [15] or very poor correlation [16-18]. Because of the poorer precision of immunoassays, larger numbers of subjects may be required to detect significant differences between populations when using immunoassays than when using mass spectrometric assays and validation by mass spectrometry of the results obtained by immunoassay, at least in a subset of samples, improves confidence in the overall conclusions.…”

Section: Measuring Lipid Peroxidation In Vivomentioning

confidence: 99%

F2-isoprostanes as an indicator and risk factor for coronary heart disease

Davies

Roberts

2011

Free Radical Biology and Medicine

Self Cite

137

110

View full text Add to dashboard Cite

Coronary heart disease (CHD) is the leading single cause of death in the United States and most Western countries, killing more than 400,000 Americans per year. Although CHD often manifests suddenly as a fatal myocardial infarction, the atherosclerosis that gives rise to the infarction develops gradually and can be markedly slowed or even reversed through pharmacological and lifestyle interventions. These same atherosclerotic processes also drive related vascular diseases such as stroke and peripheral artery disease, and individuals surviving occlusive events often develop additional complications including ischemic cardiomyopathy and heart failure. Therefore, better detection of subclinical atherosclerosis, along with more effective treatments, could significantly reduce the rate of death from CHD and related vascular diseases in the United States. In recent years, oxidation of polyunsaturated fatty acids (PUFA) in plasma lipoproteins has been postulate to be a critical step in the development atherosclerosis. If so, then monitoring lipid peroxidation should be a useful indicator of disease risk and progression. This review will focus on the evidence that specific PUFA peroxidation products, the F 2-isoprostanes, are useful biomarkers that could potentially be utilized as indicators of CHD.

show abstract

“…Although there are commercial immunoassay kits for measuring F 2 -isoprostanes, the reliability of some of these methods has been questioned. For example, some authors report a strong correlations between GC-MS F 2 -isoprostane values and values obtained by immunoassay (Basu, 1998; Sasaki et al, 2002), whereas others report significant but relatively weak correlations (i.e., r = .62, p < .02) between the two methods (Proudfoot et al, 1999). Therefore, future work is required to determine whether immunoassays for F 2 -isoprostanes can reach the sensitivity and reliability of the well-established GC-MS methods.…”

Section: Biomarkers Of Oxidative Damagementioning

confidence: 99%

Experimental Guidelines for Studies Designed to Investigate the Impact of Antioxidant Supplementation on Exercise Performance

Powers¹,

Smuder²,

Kavazis³

et al. 2010

International Journal of Sport Nutrition and Exercise Metabolism

View full text Add to dashboard Cite

Research interest in the effects of antioxidants on exercise-induced oxidative stress and human performance continues to grow as new scientists enter this field. Consequently, there is a need to establish an acceptable set of criteria for monitoring antioxidant capacity and oxidative damage in tissues. Numerous reports have described a wide range of assays to detect both antioxidant capacity and oxidative damage to biomolecules, but many techniques are not appropriate in all experimental conditions. Here, the authors present guidelines for selecting and interpreting methods that can be used by scientists to investigate the impact of antioxidants on both exercise performance and the redox status of tissues. Moreover, these guidelines will be useful for reviewers who are assigned the task of evaluating studies on this topic. The set of guidelines contained in this report is not designed to be a strict set of rules, because often the appropriate procedures depend on the question being addressed and the experimental model. Furthermore, because no individual assay is guaranteed to be the most appropriate in every experimental situation, the authors strongly recommend using multiple assays to verify a change in biomarkers of oxidative stress or redox balance.

show abstract

Enzyme Immunoassays for 15-F2t Isoprostane-M, an Urinary Biomarker for Oxidant Stress

Cited by 19 publications

References 5 publications

A longitudinal study of atrazine and 2,4‐D exposure and oxidative stress markers among iowa corn farmers

A longitudinal study of atrazine and 2,4‐D exposure and oxidative stress markers among iowa corn farmers

F2-isoprostanes as an indicator and risk factor for coronary heart disease

Experimental Guidelines for Studies Designed to Investigate the Impact of Antioxidant Supplementation on Exercise Performance

Contact Info

Product

Resources

About