Enzyme immunoassay of mumps virus in cell culture with peroxidase-labelled virus specific monoclonal antibodies and its application for determination of antibodies

Tiel, Frank H. van; Kraaijeveld, C. A.; Baller, Jfw; Harmsen, Theo; Oosterlaken, T. A. M.; Snippe, H.

doi:10.1016/0166-0934(88)90092-4

Cited by 18 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used only selected cells derived from African green (vervet) monkeys because primary African green kidney cells are known to be less sensitive than primary rhesus kidney cells for isolating paramyxoviruses [-5, 9]. Consideration was also given to previous studies which had shown some promise with Vero cells [16,17,23,26,27,33], LLC-MK2 [12,21], A-549 cells [30,34], and others [29,31,32].…”

Section: Discussionmentioning

confidence: 99%

NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses

Castells

George

Hierholzer

1990

Archives of Virology

View full text Add to dashboard Cite

Primary rhesus monkey kidney (MK) cells have long been the cells of choice for isolation and propagation of the human paramyxoviruses (parainfluenza 1, 2, 3, 4A, 4B, and mumps). However, problems with the supply and cost of MK cells and the presence of endogenous viruses, including herpes B virus and SV-5, necessitated a search for an alternative cell line. Continuous cell cultures of human origin (L132, A-549, HuT-292, HEK, G-293, G-401, A-498, A-704, CAKI-1, RD) and simian origin (LLC-MK2, BSC-1, MA-104, Vero) were evaluated for their capacity to support the growth of the human paramyxoviruses, as followed by cytopathic effect, hemadsorption, hemagglutination, and EIA. NCI-H292 (HuT-292) human lung mucoepidermoid carcinoma cells (ATCC # CRL-1848) proved to be the most sensitive line for cultivating all serotypes and strains of the paramyxoviruses. These cells were also shown to be a suitable substitute for MK in primary isolation of paramyxoviruses from clinical specimens. RPMI-1640 with 1.5 micrograms/ml trypsin was the preferred maintenance medium; alternatively, Eagle's MEM supplemented with 1.5 micrograms/ml trypsin and 0.1% ITS was satisfactory. NCI-H292 cells are a continuous line with excellent growth characteristics, although the genetic polyploidy of the cells may limit the number of passages of usable cells.

show abstract

Section: Discussionmentioning

confidence: 99%

NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses

Castells

George

Hierholzer

1990

Archives of Virology

View full text Add to dashboard Cite

show abstract

“…The mumps virus preparation contained 4 x 106 PFU/ml and a protein concentration of 0.8 mg/ml. Plaque titration and other general virological methods have been described previously (12,13).…”

Section: Methodsmentioning

confidence: 99%

“…MAb. The production and characterization of mumpsspecific monoclonal antibody (MAb) UM 10B (immunoglobulin G2a) have been described previously in another paper (12). Horseradish peroxidase (HRP) was conjugated to unpurified (ascitic fluid) MAb UM 10B by using the periodate method (8).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps

et al. 1992

View full text Add to dashboard Cite

A 50% neutralization enzyme immunoassay (Nso-EIA) was compared with an indirect enzyme-linked immunosorbent assay (ELISA) for determining mumps virus antibodies in three consecutive serum samples from 138 children vaccinated with a live mumps vaccine at the age (in years) of 1.5. By the N50-EIA, most (132 of 138) preserum samples did not show neutralizing activity. Eight to 12 weeks after vaccination, 17 of the children were still negative, but only 7 remained so after 2.5 years, resulting in a seroconversion rate of 125 of 132 (95%). Over the same period, the neutralization geometric mean titer rose from 3.6 to 9.9. By an indirect ELISA, 128 of 138 preserum samples were found negative. The early and late postvaccination sera of 8 children were ELISA negative, resulting in a seroconversion rate of 120 of 128 (94%). Only two children remained seronegative by both methods. Seven of the late postvaccination serum samples yielded noncorresponding results, reflecting 95% correlation between both methods. Due to cross-reactivity with parainfluenza viruses, the ELISA proved to be less specific, but on the other hand, it showed a greater sensitivity than the N50-EIA.

show abstract

“…For TGE infections, this technique using infected cells as a source of antigen has been used to detect total anti-TGEV antibodies (Kodama et al, 1980a) or IgA antibodies (Kodama et al, 1980b) in the serum of infected swine. For other viruses, it has been used for screening hybridomas (Russell et al, 1983), for diagnosis (Pauli et al, 1984;Groin and Bernard, 1985;Van Tiel et al, 1988) and for identification of epitopes on enveloped or naked viruses (Anderson et al, 1986;Boere et al, 1986;Oosterlaken et ai., 1989;Simkins et al, 1989). Recently, we described a quantitative fixed-cell immunoperoxidase technique for the study of surface viral antigens induced by TGEV (I"6 et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Effect of fixation on the detection of transmissible gastroenteritis coronavirus antigens by the fixed-cell immunoperoxidase technique

Tô

Bernard²

1992

Journal of Immunological Methods

View full text Add to dashboard Cite

The effect of various fixatives and detergents on the in vitro detection of the viral determinants which are expressed in swine testis cells infected with the transmissible gastroenteritis coronavirus (TGEV) was studied using a microwell immunoperoxidase technique. When compared with glutaraldehyde and formaldehyde, 0.1% paraformaldehyde was found to be the fixative of choice for the detection of these determinants on the membranes of infected cells. Among dehydrating fixatives, 80% acetone or a mixture of acetone and ethanol or of acetone, methanol and ethanol were found to be the best fixatives for the detection of these viral determinants which are expressed in infected cells. In the case of acetone, the temperature of fixation and its concentration in the fixative preparation were found to be important. The treatment of 0.05% glutaraldehyde-fixed, infected cells with 0.1% saponin or 0.1% paraformaldehyde-fixed, infected cells with 1%NP-40 led to satisfactory detection of viral determinants. Using Triton X-100 to render cells permeable, the quantities of N and M antigen detected in TGEV-infected cells prefixed with either 0.05% glutaraldehyde or 0.1% paraformaldehyde were equal to those of 80% acetone-fixed, TGEV-infected cells while the quantity of S antigen detected was diminished. The effect of other detergents such as zwittergent, empigen BB, Chaps and N-lauroylsarcosine on the detection of viral determinants was also studied.

show abstract

Enzyme immunoassay of mumps virus in cell culture with peroxidase-labelled virus specific monoclonal antibodies and its application for determination of antibodies

Cited by 18 publications

References 16 publications

NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses

NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses

Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps

Effect of fixation on the detection of transmissible gastroenteritis coronavirus antigens by the fixed-cell immunoperoxidase technique

Contact Info

Product

Resources

About