1994
DOI: 10.1248/bpb.17.440
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Enzyme Immunoassay of Motilin in Human Plasma.

Abstract: A sensitive and specific double-antibody enzyme-linked immunoassay (EIA) for detecting a motilin-like immunoreactive substance (M-IS) in human plasma has been developed. In competitive reactions, the motilin antibody was incubated with a plasma sample (or motilin standard) and beta-D-galactosidase-linked synthetic motilin. Free and antibody-bound enzymes were separated using an anti-rabbit IgG-coated immunoplate. Enzyme activity on the plate was determined by fluorophotometric analysis. This immunoassay allows… Show more

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Cited by 8 publications
(9 citation statements)
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“…Enzyme Immunoassays for Somatostatin-, CGRP-, Gastrin-, Secretin-, and Motilin-Immunoreactive Substances Peptide levels in plasma were measured using a highly sensitive enzyme immunoassay for somatostatin-, 26) CGRP-, 27) gastrin-, 28) secretin-, 29) and motilin-, 30) as previously described. The assay was performed by a delayed addition method.…”
Section: Preparation Of Plasma Extractsmentioning
confidence: 99%
“…Enzyme Immunoassays for Somatostatin-, CGRP-, Gastrin-, Secretin-, and Motilin-Immunoreactive Substances Peptide levels in plasma were measured using a highly sensitive enzyme immunoassay for somatostatin-, 26) CGRP-, 27) gastrin-, 28) secretin-, 29) and motilin-, 30) as previously described. The assay was performed by a delayed addition method.…”
Section: Preparation Of Plasma Extractsmentioning
confidence: 99%
“…19) Antiserum (Y121) was obtained from Peptide Institute, Inc. (Osaka, Japan). Motilin was labeled with b-Dgalactosidase (Boehringer Mannheim Corp., Mannheim, Germany).…”
Section: Preparation Of Plasma Extractsmentioning
confidence: 99%
“…Enzyme Immunoassay for Somatostatin-, Motilin-, Gastrin-, and VIP-IS Peptide levels in plasma were measured using a highly sensitive enzyme immunoassay for somatostatin-, 23) motilin-, 24) gastrin-, 25) and VIP-IS 26) as previously described. The assay was performed by a delayed addition method.…”
Section: Preparation Of Plasma Extractsmentioning
confidence: 99%