Enzyme immunassay of alternariol for the assessment of risk of agricultural products contamination

Буркин, А. А.; Кононенко, Г. П.

doi:10.1134/s0003683811010030

Cited by 26 publications

(15 citation statements)

References 9 publications

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“…There was a significant positive correlation between the levels of AOH and AME (r = 0.85) and between total dibenzo-α-pyrones (sum of AOH and AME) and TeA (r = 0.796) (Li and Yoshizawa, 2000). AOH was also found in feed and raw materials from Russia with the highest level of 760 µg/kg quantified in one silage sample (Burkin and Kononenko, 2011).…”

Section: Previously Reported Occurrence Results Of Alternaria Toxins mentioning

confidence: 93%

“…Direct competitive enzyme-linked immunosorbent assays (ELISA) have been used for the analysis of AAL-TA in hay, silage and mixed feed with a LOQ of 50 µg/kg (Yu et al, 1999). ELISA using polyclonal rabbit antibodies has recently been reported for AOH and applied to contaminated maize and animal feed with a LOQ of 20 µg/kg (Burkin and Kononenko, 2011). Likewise, polyclonal rabbit antibodies and monoclonal mouse antibodies have been generated against AOH and implemented in an enzyme immunoassay (EIA) with a LOD of 1-2 µg/kg (Ackermann et al, 2011).…”

Section: Immunochemical Methodsmentioning

confidence: 99%

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Scientific Opinion on the risks for animal and public health related to the presence ofAlternariatoxins in feed and food

2011

EFSA Journal

399

188

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The European Commission asked the European Food Safety Authority to review the safety of Alternaria toxins in food and feed. In addition to causing plant diseases on many crops such as cereals, oilseeds, tomatoes, apples and olives, some of these toxins are genotoxic in vitro and/or fetotoxic in rats. This opinion deals with alternariol, alternariol monomethyl ether, tenuazonic acid, iso-tenuazonic acid, altertoxins, tentoxin, altenuene and AAL-toxins (Alternaria alternata f. sp. lycopersici toxins). Two Member States provided 11,730 occurrence results in food and also data published in the scientific literature were considered. Generally these toxins were found in grains and grain-based products, tomato and tomato products, sunflower seeds and sunflower oil, fruits and fruit products, and beer and wine. The feed occurrence data (1150 results) were collected from the literature only. The knowledge on toxic effects of Alternaria toxins on farm and companion animals and occurrence data in feed were insufficient to assess the health risk for different species. For chicken there are indications that alternariol represents a health risk but it cannot be excluded that tenuazonic acid could also be of concern. Considering: (1) there are few or no relevant toxicity data on Alternaria toxins, (2) the chemical structure of several of them is known, (3) dietary exposure data exist for some of them, the Panel on Contaminants in the food chain used the threshold of toxicological concern (TTC) approach to assess the relative level of concern for dietary exposure of humans to these mycotoxins. For the genotoxic Alternaria toxins, alternariol and alternariol monomethyl ether, the estimated chronic dietary exposure exceeded the relevant TTC value indicating a need for additional toxicity data. The dietary exposure estimates for nongenotoxic tentoxin and tenuazonic acid are lower than the relevant TTC value and are considered unlikely to be a human health concern. SUMMARYAlternaria toxins are mycotoxins produced by Alternaria species that cause plant diseases on many crops. They are the principal contaminating fungi in wheat, sorghum and barley, and have also been reported to occur in oilseeds such as sunflower and rapeseed, tomato, apples, citrus fruits, olives and several other fruits and vegetables. Alternaria alternata is the most common Alternaria species in harvested fruits and vegetables, and is the most important mycotoxin-producing species. Due to their growth even at low temperature, Alternaria species are also responsible for spoilage of these commodities during refrigerated transport and storage.Alternaria species produce more than 70 phytotoxins, but a small proportion of them have been chemically characterised and reported to act as mycotoxins to humans and animals. Some toxins such as alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA) and altertoxins (ATX) are described to induce harmful effects in animals, including fetotoxic and teratogenic effects. Culture extracts of A. altern...

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Section: Previously Reported Occurrence Results Of Alternaria Toxins mentioning

confidence: 93%

Section: Immunochemical Methodsmentioning

confidence: 99%

Scientific Opinion on the risks for animal and public health related to the presence ofAlternariatoxins in feed and food

2011

EFSA Journal

399

188

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“…There are few studies around the world on the occurrence of Alternaria mycotoxins in barley grains, and when data are available, the frequency and mean concentration of the toxins reported elsewhere are lower than the ones detected in our study. For instance, Burkin and Kononenko detected 18 out of 62 barley samples positive to AOH with a mean concentration of 48 μg kg −1 in a study carried out on cereal samples from Russia, in which the detection method was enzyme‐linked immunosorbent assay (ELISA). Kütt et al .…”

Section: Discussionmentioning

confidence: 99%

Natural occurrence of Alternaria mycotoxins in malting barley grains in the main producing region of Argentina

et al. 2019

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BACKGROUND: Barley (Hordeum vulgare L.) is one of the most important cereals worldwide, and its quality is affected by fungal contamination such as species of the genus Alternaria. No information is available about the occurrence of Alternaria mycotoxins in Argentinean barley grains, which is of concern, because they can be transferred into malt and beer. The aim of this study was to analyze the occurrence of alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TeA) in malting barley grains from the main producing region of Argentina during the 2014 and 2015 growing seasons. RESULTS:The most frequent mycotoxin was AOH (64%), which was detected at higher levels (712 g kg −1 ) compared with other studies, followed by TeA (37%, 1522 g kg −1 ), while AME was present in five samples in the 2015 growing season only, with a mean of 4876 g kg −1 . A similar frequency of mycotoxin occurrence was observed in both years (80.8 vs 85.3%), but more diverse contamination was found in 2015, which was characterized by lower accumulated precipitation. Nevertheless, AOH was more frequently found in 2014 than in 2015 (80.8 and 47.1% respectively). A negative correlation between AOH concentration and temperature was observed. The susceptibility of different barley varieties to mycotoxin accumulation varied with the mycotoxin, geographical location and meteorological conditions. CONCLUSION: The results obtained in the present work represent a tool for risk assessment of exposition to these mycotoxins and could be used by food safety authorities to determine the need for their regulation. Furthermore, the establishment of a hazard analysis and critical control point (HACCP) system to minimize fungal and mycotoxin contamination in barley from farm to processing could be apply to ensure food safety.

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“…Enzyme-linked immunosorbent assay (ELISA) is a sensitive and high-throughput method for mycotoxin analysis (Kong, Xie, et al, 2017). Ackermann and Burkin developed an enzyme immunoassay for AOH determination based on monoclonal and polyclonal antibodies (Ackermann et al, 2011;Burkin & Kononenko, 2011). Compared with the ELISA method, the immunochromatographic assay is a rapid, simple, and instrument-free diagnostic tool for on-site screening with qualitative/semi-quantitative detection ability and will become the new trend for sample analysis (Kong, Liu, Song, Kuang, & Xu, 2016a).…”

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

Kong

Xie

Liu

et al. 2017

Food and Agricultural Immunology

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An immunochromatographic strip was developed for the determination of alternariol (AOH) using both semi-quantitative and quantitative methods based on a sensitive monoclonal antibody we produced. With AOH-spiked samples, the visual limit of detection for cereal and juice were 160 ng/g and 25 ng/mL, and the cut-off values were 640 ng/g and 100 ng/mL, respectively. For quantitative analysis based on a strip scan reader, the calculated limit of detection was 15.5-19.4 ng/g for cereal and 2.8-3.5 ng/mL for fruit juice. The recovery rates ranged from 116% to 125%, and 113% to 123%, respectively. Therefore, this method was effective for AOH detection and suitable for on-site detection and rapid screening of samples. ARTICLE HISTORY

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Enzyme immunassay of alternariol for the assessment of risk of agricultural products contamination

Cited by 26 publications

References 9 publications

Scientific Opinion on the risks for animal and public health related to the presence ofAlternariatoxins in feed and food

Scientific Opinion on the risks for animal and public health related to the presence ofAlternariatoxins in feed and food

Natural occurrence of Alternaria mycotoxins in malting barley grains in the main producing region of Argentina

Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

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