Enzyme immobilization in a photosensitive conducting polymer bearing azobenzene in the main chain

Abstract

A new photosensitive and thermosensitive monomer, namely bis(4-(3thienyl ethylene)-oxycarbonyl)diazobenzene (TDAZO), was synthesized. The photochemical and thermal cis-trans isomerization of the monomer has been investigated. The rate constants of the photoisomerization of TDAZO in ACN and DCM were 0.195 and 0.308 min-1 , respectively. For spectroelectrochemical investigation and enzyme immobilization application, TDAZO copolymerized with thiophene and pyrrole. Electrochemical and spectroelectrochemical properties of P(TDAZO-co-Th) were investigated and invertase was immobilized in P(TDAZOco-Py) copolymer. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the TDAZO. Optimum conditions for this electrode, such as pH, temperature, kinetic parameters (K m and V max) and operational stability were investigated. Kinetic parameters invertase-immobilized in copolymer were smaller than free enzyme. The optimum operational temperature was 10°C higher for immobilized enzyme than that of the free enzyme. Due to

Nanokarbon ile Desteklenmiş Piren Sübstitüye İletken Polimerin Elektrokimyasal Karakterizasyonu

“…Kompozit malzemeler, organik iletken polimerler ile çok farklı özelliklere sahip maddelerin kombinasyonu ile yeni araştırma alanlarında, performans ve kaliteyi arttırmak için tasarlanmıştır [18][19][20][21]. Bu nedenle, çeşitli bilim dallarından araştırmacılar, birbirinden tamamen farklı yapıya sahip hibrit organik-inorganik polimerik materyallerle ilgilenmektedir.…”

An update on smart biocatalysts for industrial and biomedical applications

“…After conjugation, the kinetic parameters of the enzyme were unchanged; however, an increase of 108C in the optimum temperature of the enzyme was observed. The interactions between the enzyme and the diazo group of the polymer enhanced the thermal stability, pH and reusability of the enzyme several-fold [87].…”

Experimental and Theoretical Investigations of an Electrochromic Azobenzene and 3,4‐Ethylenedioxythiophene‐based Electrochemically Formed Polymeric Semiconductor

“…Early attempts to obtain polymeric azobenzenes in the main polymer chain resulted in black or brown products of low molecular weight and poor solubility. [21,22] In this study, we report a novel electrochromic polymer, which contains azobenzene and 3,4-ethylenedioxythiophene (EDOT) moieties in the polymer backbone. [18] A successful approach to include azobenzene unit into conjugated polymer main chain was demonstrated by Zaytungul et al by coupling azobenzene with thiophene and obtaining a polymer which exhibited chromic response to organic acids.…”

“…On the other hand, conjugated polymers based on thiophene have attracted great interest towards their application in various semiconducting devices where the electronic properties of corresponding materials can be manipulated through doping or chemical modification. [21,22] In this study, we report a novel electrochromic polymer, which contains azobenzene and 3,4-ethylenedioxythiophene (EDOT) moieties in the polymer backbone. Electrochemical synthesis as well as electrochemical and electrochromic properties of novel conjugated polymer were investigated on transparent ITO electrodes and the obtained experimental data was supplemented and further explained by using density functional theory calculations.…”