Enzyme-histochemical Differentiation of Neuroglia and Micro ia: A Contribution to the Cytogenesis of Microglia and Globoid Cells

Oehmichen, M.

doi:10.1016/s0344-0338(80)80272-x

Cited by 24 publications

(11 citation statements)

References 108 publications

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“…In some of these studies, NSE activity has also been demonstrated in cells that are not associated with the blood, including kidney and intestinal epithelial cells (Stadnyk et al, 1990;Akikusa et al, 1984). However, in the mammalian CNS, activity has been reported only in activated microglia and macrophages (Giulian et al, 1989;Ling et al, 1982;Ferrer and Sarmiento, 1980; Oehmichen, 1980; Schelper and Adrian, 1980). Therefore, our observation that astrocytes in the goldfish brain express strong NSE activity is totally unexpected and demonstrates a unique characteristic of the teleost brain.…”

Section: Discussionsupporting

confidence: 46%

“…In addition, it has been particularly useful in identifying macrophages in sections of inflamed tissues (Akikusa et al, 1984) including the brain (Giulian et al, 1989;Ferrer and Sarmiento, 1980 Oehmichen, 1980; Schelper and Adrian, 1980). In some of these studies, NSE activity has also been demonstrated in cells that are not associated with the blood, including kidney and intestinal epithelial cells (Stadnyk et al, 1990;Akikusa et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

“…To our surprise, we discovered that the NSE reaction densely labeled radial glia in the brains of these animals. In all other reports of NSE activity in the (mammalian) CNS, the only cells that label are reactive mononuclear cells (Giulian et al, 1989;Ferrer and Sarmiento, 1980;Oehmichen, 1980;Schel-per and Adrian, 1980). Therefore, our observations in the goldfish are unique and we present them here in some detail.…”

Section: Introductionmentioning

confidence: 92%

“…Consequently, it has found wide use in studies of these ' Supported by the Medical Research Council of Canada and by the cells in a variety of tissues (Stadnyk et al, 1990;Sannes and Randell, 1985;Verspaget and Becken, 1985;Akikusa et al, 1984;Soufleris et al, 1983). In addition, the NSE reaction has been used in mammals to examine reactive mononuclear cells in the damaged central nervous system (CNS) (Giulian et al, 1989;Ferrer and Sarmiento, 1980;Oehmichen, 1980;Schelper and Adrian, 1980) as well as ameboid microglia in the developing brain (Ling et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

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Nonspecific esterase activity in astrocytes of the goldfish brain.

Pepperell¹,

Levine²

1996

J Histochem Cytochem.

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We examined the distribution of nonspecific esterase (NSE) activity in the brain of the goldfish, Carawius auracus, and found that the enzyme is expressed at high levels in cells that appeared to be radial astrocytes. Several instances in which neurons expressed NSE activity were also seen. To confirm the identity of the radial profiles as astrocgtes, similar sections were labeled with antiserum against goldfiih glial fibrillary acidic protein (GFAP). The concordance between the NSE and the anti-GFAP data in both the visual system and the telencephalon was essentially complete, confirming that the NSE reaction was labeling astrocytes in these structures. The two methods also gave similar results in both the cerebellum and the vagal lobes, although the concordance between them in these instances was somewhat less complete.

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Section: Discussionsupporting

confidence: 46%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nonspecific esterase activity in astrocytes of the goldfish brain.

Pepperell¹,

Levine²

1996

J Histochem Cytochem.

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“…Recently, several investigators have tried to prove a relationship between monocytes or macrophages and microglia by means of immunohistochemistry (OEHMICHEN et al, 1979;OEHMICHEN, 1980;FUJITA et al, 1981). However, their immunohistochemical data were negative using anti-macrophage and anti-monocyte sera.…”

Section: Discussionmentioning

confidence: 99%

Distribution and fate of macrophagic ameboid cells in the rat brain.

Imamoto

Fujiwara

Nagai

et al. 1982

Arch. Histol. Jap., Arch. Histol. Jpn

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Regulation of the rat oligodendroglia cell line OLN‐93 by antisense transfection of butyrylcholinesterase

Robitzki

Döll

Richter‐Landsberg

et al. 2000

Glia

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Butyrylcholinesterase (BChE) is a glial cell marker with unknown function. For neuroepithelial cells, BChE has been shown to regulate cell division and expression of the postmitotic marker acetylcholinesterase (AChE), while similar studies are lacking for glial cells. By transducing an antisense‐5′BChE cDNA expression vector via calcium phosphate precipitation, we have analyzed the effect of BChE inhibition on proliferation and differentiation of rat oligodendroglia‐derived OLN‐93 cells. OLN‐93 cells were chosen because they are highly proliferative, while expressing markers of differentiated oligodendrocytes (Richter‐Landsberg and Heinrich, 1996). First, we established that OLN‐93 cells do express BChE protein, albeit chiefly in an inactive state, and that BChE was decreased by antisense‐5′BChE transfection. Cell proliferation was also strongly diminished, protein kinase C (PKCα) was upregulated, and expression of cytoskeletal and cell surface proteins was altered. In particular, immunoreactivities of the intermediate filament proteins vimentin and the cell adhesion protein F11 were detected, indicating that BChE‐inhibited OLN‐93 cells have shifted toward an astrocytic phenotype. These data support a role of the glia marker BChE in CNS glial cell proliferation and differentiation, achieved via a nonenzymatic mechanism. The possible biomedical impact of BChE protein, e.g., on CNS nerve regeneration, is briefly discussed. GLIA 31:195–205, 2000. © 2000 Wiley‐Liss, Inc.

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Enzyme-histochemical Differentiation of Neuroglia and Micro ia: A Contribution to the Cytogenesis of Microglia and Globoid Cells

Cited by 24 publications

References 108 publications

Nonspecific esterase activity in astrocytes of the goldfish brain.

Nonspecific esterase activity in astrocytes of the goldfish brain.

Distribution and fate of macrophagic ameboid cells in the rat brain.

Regulation of the rat oligodendroglia cell line OLN‐93 by antisense transfection of butyrylcholinesterase

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