Enzyme-free electrochemical biosensor based on amplification of proximity-dependent surface hybridization chain reaction for ultrasensitive mRNA detection

Cheng, Yu-Hong; Liu, Sijia; Jiang, Jian‐Hui

doi:10.1016/j.talanta.2020.121536

Cited by 21 publications

(5 citation statements)

References 39 publications

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“…In addition to the strategies mentioned above, other HCR technologies have been developed. A recent study by Cheng et al (2021) used proximity-dependent surface hybridization chain reactions to design an electrochemical biosensor for miRNA detection. MiRNA triggered HCR and the formation of duplexes with single-stranded dangling tails, which were labeled with ferrocene (Fc) and could be used to identify the sequence fixed on the electrode.…”

Section: Dna-based Biosensors Integrated With Hcr or Chamentioning

confidence: 99%

Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly

et al. 2023

Front. Chem.

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As isothermal, enzyme-free signal amplification strategies, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) possess the advantages such as high amplification efficiency, excellent biocompatibility, mild reactions, and easy operation. Therefore, they have been widely applied in DNA-based biosensors for detecting small molecules, nucleic acids, and proteins. In this review, we summarize the recent progress of DNA-based sensors employing typical and advanced HCR and CHA strategies, including branched HCR or CHA, localized HCR or CHA, and cascaded reactions. In addition, the bottlenecks of implementing HCR and CHA in biosensing applications are discussed, such as high background signals, lower amplification efficiency than enzyme-assisted techniques, slow kinetics, poor stability, and internalization of DNA probes in cellular applications.

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Section: Dna-based Biosensors Integrated With Hcr or Chamentioning

confidence: 99%

Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly

et al. 2023

Front. Chem.

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“…This can be used to covalently attach adhesion layers for crosslinkers and enzymes in biosensors, for instance, in the application of mRNA detection. [36] Moreover, thiol chemistry can be used to make excellent sticking layers onto which gold can be deposited, presenting a good alternative to underlayers like Cr. [37] Due to its electrocatalytic properties, gold can be used for nonenzymatic electrochemical sensing of glucose.…”

Section: Metals and Their Compoundsmentioning

confidence: 99%

State‐of‐the‐Art Electronic Materials for Thin Films in Bioelectronics

Gablech

Głowacki

2023

Adv Elect Materials

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This review is dedicated to electronics materials enabling thin‐film‐based neural interface and bioelectronics devices. First‐generation bioelectronic medicine devices feature hand‐crafted bulk interface electrodes, wires and interconnects, and insulators. This review discusses how modern materials science, especially know‐how repurposed from semiconductor and microdevice technologies, enables next‐generation bioelectronics. Those are divided into two subgroups: second and third generation. The former refers to rigid microscaled devices, while the latter is defined as soft, ultrathin, and flexible microdevices. A critical assessment of different biointerface electrodes, conductors for interconnects, and insulators for substrates, passivation, and encapsulation layers is made. The goal is not to give an exhaustive account of every use‐example of given materials, but to point out specific aspects that are relevant to making the right choices for materials for a given device or application. Unique advantages of specific materials are highlighted, while also focusing on weaker points and caveats that those materials may have. The goal is to have an up‐to‐date handbook for persons entering the field which also points out tips and tricks as well as challenging problems that researchers can be inspired to confront and overcome.

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“…Cheng et al designed a biosensor for the typical tumor marker surviving mRNA using cyclic voltammetry to achieve low-cost detection of cancer. [40] Sheng et al developed a dual isothermal amplified electrochemical biosensor based on CHDC and CRISPR/Cas13a to detect TTF-1 mRNA detecting non-small cell lung cancer successfully. [41] However, due to their defects of low abundance, short half-life, and complex types, the current understanding of mRNAs is still limited.…”

Section: Mrnasmentioning

confidence: 99%

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

Sun

Gao

et al. 2023

Adv Materials Technologies

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Exosomes are a type of small extracellular vesicles carrying proteins, nucleic acids, and lipids. Because their expression levels are highly correlated with tumor development, exosomes are regarded as non‐invasive biomarkers for cancer diagnosis. Therefore, many exosome detection techniques are developed to provide a new way for cancer diagnosis. However, conventional techniques require the isolation of exosomes from body fluids before analysis, which usually relies on complex equipment and cumbersome operations. In addition, complex procedures may introduce impurities and thus affect the accuracy of the test. As an emerging analysis technology, microfluidic overcomes these shortcomings by integrating a microchannel for sample transport isolation and an exosome analysis module, showing great prospects in cancer diagnosis. This review will provide an overview of microchannel fabrication and analysis methods with outstanding compatibility for different microfluidic types, with a particular focus on microfluidic‐based exosome analysis methods that have the potential for clinical application.

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Enzyme-free electrochemical biosensor based on amplification of proximity-dependent surface hybridization chain reaction for ultrasensitive mRNA detection

Cited by 21 publications

References 39 publications

Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly

Recent progress in the development of DNA-based biosensors integrated with hybridization chain reaction or catalytic hairpin assembly

State‐of‐the‐Art Electronic Materials for Thin Films in Bioelectronics

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

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