Enzyme free detection of staphylococcal enterotoxin B (SEB) using ferrocene carboxylic acid labeled monoclonal antibodies: an electrochemical approach

Sharma, Arun; Rao, Venkat K.; Kamboj, Dev Vrat; Gaur, Ritu; Shaik, Mahabul; Shrivastava, Anchal Roy

doi:10.1039/c5nj03460d

Cited by 21 publications

(14 citation statements)

References 38 publications

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“…Some of the compounds detected electrochemically using graphene and graphene‐based materials as electrode material include dopamine, glucose, H 2 O 2 , haemoglobin, DNA, glycoproteins, cancer cells, adenosine triphosphate (ATP), angiogenin, K562 leukemia cancer cells, human epidermal growth factor receptor 2; electrochemical DNA biosensor is developed for quantitative detection of breast cancer 1 (BRCA1) gene, impedimetric immunosensor for the direct detection of bacterial toxin botulinum has been developed using gold nanoparticles/graphene–chitosan composite, staphylococcal enterotoxin B has been detected using Au–Gr–Cs‐modified glassy carbon electrode, Au nanoparticles (NPs) nanocomposite and polypyrene electrochemically reduced graphene oxide flm‐coated disposable screen‐printed carbon electrode based on the voltammetric immunosensing method for fast, sensitive, and selective detection of two mycotoxins, deoxynivalenol, and fumonisin B1 (FB1) in spiked corn samples . Another impedimetric immunosensor has been developed for the rapid detection of ochratoxin (OTA) in food samples by immobilizing both anti‐OTA antibody and amine‐terminated dendrimer on the graphene oxide sheets .…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Graphene and Graphene‐Based Materials in Biomedical Science

Swetha

Manisha

Prasad

2018

Part & Part Syst Charact

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Graphene and its composite materials are very important in many disciplines of science and have been used enormously by researchers since their discovery in 2004. These are a new group of compounds, and are also wonderful model systems for quantum behavior studies. Their properties like exceptional conductivity, biocompatibility, surface area, mechanical strength, and thermal properties make them rising stars in the scientific community. Graphene and its composite compounds are utilized widely in different medical applications, for example, biosensing of biological compounds responsible for disease development, bioimaging of various cells, tissues, microorganisms, animal models, etc. In addition, they are used for enhancing and supporting the stem cell differentiation, i.e., regenerative medicine for regeneration studies of various human organs, tissue engineering in biology for the development of carrier materials, as well as in bone reformation. This review focuses on the modification procedure involved in the fabrication of graphene‐based biomaterials for various applications and recent developments in research related to graphene and graphene‐based materials in biosensing, optical sensing, gas sensing, drug, gene, protein delivery, tissue engineering, and bioimaging. In addition, the potential toxicological effects of graphene‐based biomaterials are discussed.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Graphene and Graphene‐Based Materials in Biomedical Science

Swetha

Manisha

Prasad

2018

Part & Part Syst Charact

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“…The role of the 2D nanomaterials including graphene, MoS 2 , and MOF was mainly as large-area support facilitating the efficient immobilization of the biorecognition elements. To enhance the sensitivity of the assay, the electrical conductivity of the sensor was improved by using nanomaterial composites, typically with Au nanostructures [ 128 , 129 ], or by using enzyme-based signal amplification systems [ 106 , 130 ]. Meanwhile, the preferred detection methods were DPV and EIS with [Fe(CN) 6 ] 4−/3− as a redox probe.…”

Section: Biosensing Applications Of 2d Nanomaterials For Food and Environmental Analysismentioning

confidence: 99%

Two-Dimensional Nanostructures for Electrochemical Biosensor

Khan

Radoi

Rashid

et al. 2021

Sensors

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Current advancements in the development of functional nanomaterials and precisely designed nanostructures have created new opportunities for the fabrication of practical biosensors for field analysis. Two-dimensional (2D) and three-dimensional (3D) nanomaterials provide unique hierarchical structures, high surface area, and layered configurations with multiple length scales and porosity, and the possibility to create functionalities for targeted recognition at their surface. Such hierarchical structures offer prospects to tune the characteristics of materials—e.g., the electronic properties, performance, and mechanical flexibility—and they provide additional functions such as structural color, organized morphological features, and the ability to recognize and respond to external stimuli. Combining these unique features of the different types of nanostructures and using them as support for bimolecular assemblies can provide biosensing platforms with targeted recognition and transduction properties, and increased robustness, sensitivity, and selectivity for detection of a variety of analytes that can positively impact many fields. Herein, we first provide an overview of the recently developed 2D nanostructures focusing on the characteristics that are most relevant for the design of practical biosensors. Then, we discuss the integration of these materials with bio-elements such as bacteriophages, antibodies, nucleic acids, enzymes, and proteins, and we provide examples of applications in the environmental, food, and clinical fields. We conclude with a discussion of the manufacturing challenges of these devices and opportunities for the future development and exploration of these nanomaterials to design field-deployable biosensors.

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“…The following examples of sandwich type amperometric immunosensors will be presented herein (Figure 3). Another strategy for signal amplification has been proposed by Sharma et al [47]. The bionanocomposite containing graphene-chitosan-gold nanoparticles capturing antibodies has been used for the construction of electrochemical sandwich immunoassay using ferrocene-labelled detection antibodies for the detection of Staphylococcus enterotoxin B (SEB).…”

Section: Sandwich Immunosensors Based On Antibody-electroactive Probementioning

confidence: 99%

Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

Kondzior

Grabowska

2020

Sensors

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Suitable immobilization of a biorecognition element, such as an antigen or antibody, on a transducer surface is essential for development of sensitive and analytically reliable immunosensors. In this review, we report on (1) methods of antibody prefunctionalization using electroactive probes, (2) methods for immobilization of such conjugates on the surfaces of electrodes in electrochemical immunosensor construction and (3) the use of antibody-electroactive probe conjugates as bioreceptors and sensor signal generators. We focus on different strategies of antibody functionalization using the redox active probes ferrocene (Fc), anthraquinone (AQ), thionine (Thi), cobalt(III) bipyridine (Co(bpy)33+), Ru(bpy)32+ and horseradish peroxidase (HRP). In addition, new possibilities for antibody functionalization based on bioconjugation techniques are presented. We discuss strategies of specific, quantitative antigen detection based on (i) a sandwich format and (ii) a direct signal generation scheme. Further, the integration of different nanomaterials in the construction of these immunosensors is presented. Lastly, we report the use of a redox probe strategy in multiplexed analyte detection.

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Enzyme free detection of staphylococcal enterotoxin B (SEB) using ferrocene carboxylic acid labeled monoclonal antibodies: an electrochemical approach

Abstract: We report a ferrocene based electrochemical immunosensor for staphylococcal enterotoxin B.

Cited by 21 publications

References 38 publications

Graphene and Graphene‐Based Materials in Biomedical Science

Graphene and Graphene‐Based Materials in Biomedical Science

Two-Dimensional Nanostructures for Electrochemical Biosensor

Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

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