Enzyme electrode for the determination of bilirubin

Fortuney, A.; Guilbault, George G.

doi:10.1002/elan.1140080306

Cited by 28 publications

(14 citation statements)

References 15 publications

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“…A graphite epoxy electrode incorporating BOD has been described by Wang and Ozsoz [6]. Recently an enzymic method based on the amperometric detection of hydrogen peroxide using immobilized bilirubin oxidase is described [7], however, according to the literature hydrogen peroxide is not produced in the course of the enzymic reaction [1,2,8]. A ®ber optic¯uorescence biosensor [9] and a multilayer enzyme electrode [10] have also been reported.…”

Section: Introductionmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

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An enzymic method for the determination of bilirubin is described, based on the indirect electrochemical monitoring of bilirubin via its reaction with oxygen to biliverdin in the presence of bilirubin oxidase. Amperometric signals due to oxygen depletion were taken by means of an oxygen electrode incorporating an immobilized bilirubin oxidase membrane. The calibration plot shows linearity up to 2.0610 74 M. This concentration range corresponds to elevated bilirubin concentrations in adults and in newborn infants. The RSD of the method is 3.1 % (1.0610 74 M bilirubin, n 6). The method is interference free, fast (`3min) and easy to perform. The membrane retains almost 80 % of its initial activity after 30 successive measurements.

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Section: Introductionmentioning

confidence: 99%

An Enzymic Method for the Determination of Bilirubin Using an Oxygen Electrode

2000

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“…e l s e v i e r . c o m / l o c a t e / t s f electrochemistry of BR in aqueous solutions [12,14,15,20]. In all these works, a BR stock solution was prepared by dissolving it in a basic solvent.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Unfortunately this enzyme is highly unstable. Some strategies have been employed to overcome this problem (conductive polymers [12], mediators [13] and cross-linking agents [14,15] as well as multilayer enzyme networks [13]). Alternatively, the instability related to the enzymatic detection could be solved by exploiting the spontaneous conversion of BR to BV, occurring once suitable potentials are applied.…”

Section: Introductionmentioning

confidence: 99%

Superior sensing performance of multi-walled carbon nanotube-based electrodes to detect unconjugated bilirubin

et al. 2013

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The direct electrochemical behaviour of bilirubin in the physio-pathological concentration range and at physiological pH was investigated by cyclic voltammetry. Nanostructured electrodes with a thin film of multi-walled carbon nanotubes exhibited a higher sensing performance than bare electrodes. The detection limit obtained with nanostructured electrodes (4.2 ± 0.1 μM) allows the detection of both normal and pathological levels of bilirubin. Due to its sparse solubility in aqueous solvents, in human fluids bilirubin is found in the form of soluble complex with albumin. Therefore, the nanostructured-sensor response was studied in presence of different concentrations of this protein. A signal weakening was observed with increasing concentrations of albumin due to the decrease of free bilirubin. Finally, bilirubin detection was tested at concentrations typical of newborn jaundice (200-500 μM) and in the presence of normal albumin levels. A detection limit of 9.4 ± 0.3 μM was identified. Since this value is below the minimum critical bilirubin concentration for newborns, our sensor, modified with a thin film of carbon nanotubes, could potentially be used for bilirubin detection in cases of newborn jaundice.

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“…The methods can be divided into two groups, viz., the direct and indirect methods. The former include determinations based either on a diazo reaction [6], a spectrophotometric detection [7,8], an enzymatic oxidation to colorless compounds by horseradish peroxidase [9], a fiber-optic sensor estimation [10], or an enzyme electrode method [11]; the latter involve chromatographic separation based on Sephadex [12], TLC [13], and HPLC [14] systems. The major problems with the existing methods are the occurrence of interference with the direct methods and the disturbance of equilibrium between free bilirubin and albumin during the separation process in the indirect methods.…”

Section: Introductionmentioning

confidence: 99%