Enzyme Chemotaxis on Paper-based Devices

Ilacas, Grenalynn; Basa, Alexis; Sen, Ayusmen; Gomez, Frank A.

doi:10.2116/analsci.34.115

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…Since its introduction by the Whitesides group in 2007, microfluidic paper-based analytical devices (PADs) have become an expanding research field with applications encompassing a breadth of scientific disciplines [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. A wide array of fabrication processes has been detailed in the literature as have detection techniques focused mainly on colorimetric types.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

et al. 2018

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A reusable and simple to fabricate electrochemical sensor for the detection of glucose and acetylthiocholine using thread‐based electrodes and nylon thread is described. The fabrication of the device consisted of two steps. First, three nylon‐based electrodes (reference, working, and counter) were painted with one layer of conductive inks (silver and carbon ink, or silver/silver chloride ink). The electrodes were taped onto parafilm, and a piece of white nylon thread was wrapped around each electrode connecting the three electrodes. For the glucose system, a PBS solution containing glucose oxidase (GOx) (10 mg/mL), and potassium ferricyanide (K3[Fe(CN)6]) (10 mg/mL) as mediator, was dried onto the thread, and increasing concentrations of glucose (0–15 mM) was added to the thread and measured by cyclic voltammetry (CV). The current output from the glucose oxidation was proportional to the concentration of glucose. For the second system, a solution of acetylcholinesterase (AChE) (0.08 U/mL) in PBS was added to the nylon thread, and increasing concentrations of acetylthiocholine (ATC) (0–9.84 mg/mL) was added and measured by CV. The current output from the oxidation of thiocholine (produced by AChE reacting with ATC) was proportional to the concentrations of ATC added to the thread. From both systems, a graph of current output versus substrate concentration was produced and fitted with a linear regression line that gave R2 values of 0.985 (GOX/glucose) and 0.995 (AChE/ATC).

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Section: Introductionmentioning

confidence: 99%

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

et al. 2018

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“…Effective anti‐diabetic drugs, dietary modifications, and exercise are necessary to maintain the glucose level in diabetic patients. Self‐test kits or rapid biosensors become essential for daily checking of glucose levels for proper treatment . Since the introduction of the first PAD by Whitesides group in 2007 , many developments have been emerged to increase the applications of PADs , including for glucose assays.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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“…(6)(7)(8)(9)(10)(11) These devices have been used in diverse medical and clinical applications. (12)(13)(14)(15)(16)(17)(18)(19) Along with their fabrication, different modifications have been proposed to make the output of microPADs more meaningful, (20)(21)(22) but a few issues such as readability still remain to be resolved fully. For example, after taking a biofluid sample (e.g., serum, urine, etc.…”

Section: Introductionmentioning

confidence: 99%

FrameQR®-code-embedded Paper Sensors: One-click Screening Solution to Analyze Colorimetric Output and On-chip Test and Patient Information

Nishat¹,

Asaf²,

Awan³

2021

Sensors and Materials

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Enzyme Chemotaxis on Paper-based Devices

Cited by 13 publications

References 33 publications

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine

Recent applications of paper‐based point‐of‐care devices for biomarker detection

FrameQR®-code-embedded Paper Sensors: One-click Screening Solution to Analyze Colorimetric Output and On-chip Test and Patient Information

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