Enzyme capturing and concentration with mixed matrix membrane adsorbers

Saiful, Saiful; Borneman, Zandrie; Weßling, Matthias

doi:10.1016/j.memsci.2006.01.045

Cited by 71 publications

(47 citation statements)

References 45 publications

(58 reference statements)

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“…The porosity of the membrane was calculated to be approximately 74%, which is quite similar to membranes prepared by Wessling's group (Avramescu et al, 2003a;Saiful et al, 2006). The thickness of the membrane after freeze drying was measured by a micrometer to be approximately 200 mm.…”

Section: Structure Of Mixed Matrix Membranessupporting

confidence: 64%

“…Intuitively, one would expect the binding to increase as the pH of binding deviates upwards from the pI of the protein, as the protein would be more highly charged at higher pH values. However, several authors have reported similar results where the highest binding capacity occurs near the pI of the protein (Saiful et al, 2006;Yamamoto and Ishihara, 1999) and it is recognized now that ion exchange properties of proteins cannot be fully explained by the net charge concept alone (Kacar and Arica, 2001). Changes of buffer pH can alter the charge distribution, charged group in the contact regions, conformational structure, and zeta potential of the surface of proteins.…”

Section: Structure Of Mixed Matrix Membranesmentioning

confidence: 80%

“…Membrane porosity was measured according to previously published methods (Avramescu et al, 2003a;Saiful et al, 2006), with porosity, e (%), defined as.…”

Section: Preparation Of Mixed Matrix Membranesmentioning

confidence: 99%

“…Sometimes harsh chemical and physical conditions must be used, which can cause undesirable and irreversible changes in the membrane structure (Liu and Bai, 2006). Recently, Wessling's group at the Membrane Technology Group, The Netherlands, introduced a new method for producing ion exchange membranes, which seems to be versatile and attractive in terms of preparation and performance, called mixed matrix membrane (MMM) chromatography (Avramescu et al, 2003a,b;Saiful et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…The concept is quite simple and it is easy to prepare such mixed matrix membranes while still giving a performance that is competitive with current chromatographic membranes. Wessling et al prepared MMM adsorbers using an ethylene vinyl alcohol (EVAL) base membrane with several types of lewatit cation and anion exchange resins and successfully applied these to the separation of bovine serum albumin (BSA), lysozyme and bilirubin (Avramescu et al, 2003b(Avramescu et al, , 2004Saiful et al, 2006). Although this type of ion exchange MMM adsorber was first reported in 2003, little attention has been given to it for industrial applications.…”

Section: Introductionmentioning

confidence: 99%

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Fractionation of β‐Lactoglobulin from whey by mixed matrix membrane ion exchange chromatography

Saufi

Fee

2009

Biotech & Bioengineering

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Mixed matrix membranes (MMMs), which incorporate adsorptive particles during membrane casting, can be prepared simply and have performances that are competitive with other membrane chromatography materials. The application of MMM chromatography for fractionation of beta-Lactoglobulin from bovine whey is described in this article. MMM chromatography was prepared using ethylene vinyl alcohol polymer and lewatit anion exchange resin to form a flat sheet membrane. The membrane was characterized in terms of structure and its static and dynamic binding capacities were measured. The optimum binding for beta-Lactoglobulin was found to be at pH 6.0 using 20 mM sodium phosphate buffer. The MMM had a static binding capacity of 120 mg/g membrane (36 mg/mL membrane) and 90 mg/g membrane (27 mg/mL membrane) for beta-Lactoglobulin and alpha-Lactalbumin, respectively. In batch fractionation of whey, the MMM showed selective binding towards beta-Lactoglobulin compared to other proteins. The dynamic binding capacity of beta-Lactoglobulin in whey solution was about 80 mg/g membrane (24 mg beta-Lac/mL of MMM), which is promising for whey fractionation using this technology. This is the first reported application of MMM chromatography to a dairy feed stream.

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Section: Structure Of Mixed Matrix Membranessupporting

confidence: 64%

Section: Structure Of Mixed Matrix Membranesmentioning

confidence: 80%

“…Membrane porosity was measured according to previously published methods (Avramescu et al, 2003a;Saiful et al, 2006), with porosity, e (%), defined as.…”

Section: Preparation Of Mixed Matrix Membranesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fractionation of β‐Lactoglobulin from whey by mixed matrix membrane ion exchange chromatography

Saufi

Fee

2009

Biotech & Bioengineering

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Industrial Application of Membrane Chromatography for the Purification of Enzymes

Beutel

Villain

Scheper

2016

Applied Biocatalysis: From Fundamental Science to Industrial Applications

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Preparation and characterization of poly(styrene‐co‐divinylbenzene)/fiberglass cation‐exchange composites

Huang

Líu

Wang

2010

J of Applied Polymer Sci

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Cation-exchange composite materials were prepared by the sulfonation of the copolymer of styrene (St) and divinylbenzene (DVB) coated on glass fibers previously surface-treated by a silane coupling agent. The results show that the surface treatment of the glass fibers by the coupling agent c-(methacryloyloxyethyl) trimethoxysilane led to a vinyl functionalized fiberglass surface, which served to covalently bond the copolymer to the fiberglass. Increasing the amount of the coupling agent increased the degree of coating of the copolymer on the fiberglass. A cation-exchange composite with an exchange capacity of 4.08 mmol/g of resin was prepared by the copolymerization of St and DVB at a weight ratio of 95 : 5 for 30 min; this was followed by sulfonation of the copolymer at 100C for 12 h.

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Enzyme capturing and concentration with mixed matrix membrane adsorbers

Cited by 71 publications

References 45 publications

Fractionation of β‐Lactoglobulin from whey by mixed matrix membrane ion exchange chromatography

Fractionation of β‐Lactoglobulin from whey by mixed matrix membrane ion exchange chromatography

Industrial Application of Membrane Chromatography for the Purification of Enzymes

Preparation and characterization of poly(styrene‐co‐divinylbenzene)/fiberglass cation‐exchange composites

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