Enzyme-Based Fiber Optic Sensor for Glucose Determination

Abdel-Latif, Monzir S.; Aa, Suleiman; Guilbault, George G.

doi:10.1080/00032718808071922

Cited by 32 publications

(10 citation statements)

References 7 publications

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“…However, there are a number of chemical sensors with selective layers which are suitable for catheters, e.g., glucose [126,127], lactate [128], sodium [129], ammonium and nitrates [130]. All of these sensors used electrical readout, but these can also be achieved using optical methods [131].…”

Section: Selective Chemical Measurementmentioning

confidence: 99%

Sensors for Catheter Applications

2003

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Minimally invasive techniques enable diagnostics and surgery to be performed through a small incision. This greatly reduces the damage to healthy tissue leading to faster recovery times for the patient. However, the surgeon is deprived of the sensory feedback available with normal surgical techniques. Miniaturised sensors can provide this information about location, diagnostics and the treatment. This application severely limits the size of the device, with some catheters being less than 1 mm in diameter. Catheters are usually defined in multiples of 1/3 mm or 1 french. Further important issues are bio-compatibility, safety and the ability of the sensor to withstand the working environment. Sensors able to meet these demands are improving the efficiency of operations and are opening new opportunities for minimally invasive techniques.

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Section: Selective Chemical Measurementmentioning

confidence: 99%

Sensors for Catheter Applications

2003

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“…; Hu, K.-R.; Liu, J.-R.; Ma, H.-J. Fengxi Huaxue 1982, 70 (4), 232. (5) Shi, H.-M.; Cui, W.-C. Fengxi Huaxue 1982, 70 (9), 561.…”

Section: Literature Citedunclassified

Avidin-biotin coupling as a general method for preparing enzyme-based fiber-optic sensors

Luo¹,

Walt²

1989

Anal. Chem.

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A general method for preparing enzyme-based fiber-optic sensors Is described. Three different sensors, capable of detecting penicillin, organic esters, and urea, were developed based on this method. Each fiber was constructed first with a layer of polymer containing pendant amino groups that react further with succinlmidyl-derlvatlzed biotin. Later biotin and fluorescein-labeled enzymes were Introduced to the fiber through a sandwich blotln-avidln-biotln Interaction. The substrate concentration is detected Indirectly through the -sensltlve dye, fluorescein, which responds to the microenvironmental pH changes produced by the enzyme catalyzed reactions. The response times for the penicillin, organic ester, and urea optrodes are less than 90, 30, and 70 s, respectively. Each sensor has a detection limit of 0.1 mM and responds to substrate concentrations over 2 orders of magnitude.

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“…Most of these use electrochemical methods 12,13 . Absorption based optical fiber glucose sensors have also been reported in literature 11 .…”

Section: Introductionmentioning

confidence: 98%

“…Therefore, the detection and monitoring of glucose level in blood at regular intervals is essential. Many studies have been performed for the detection of glucose [11][12][13] . Most of these use electrochemical methods 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance based fiber optic glucose biosensor

2012

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A surface plasmon resonance (SPR) based fiber optic biosensor has been fabricated and characterized for the detection of blood glucose. Optical fiber sensor was fabricated by first coating a 50 nm thick gold film on the bare core of optical fiber and then immobilizing glucose oxidase (GOx) over it. Aqueous glucose solutions of different concentrations were prepared. To mimic the blood glucose levels, the concentration of glucose solutions were kept equal to that in human blood. The refractive indices of these sample solutions were equal to that of water up to third decimal place. SPR spectra for the sensor were recorded for these glucose solutions. When the glucose comes in contact to glucose oxidase, chemical reactions take place and as a result, the refractive index of the immobilized GOx film changes, giving rise to a shift in the resonance wavelength. Unlike electrochemical sensors, the present sensor is based on optics and can be miniaturized because of optical fiber. The present study provides a different approach for blood glucose sensing and may be commercialized after optimization of certain parameters.

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Enzyme-Based Fiber Optic Sensor for Glucose Determination

Cited by 32 publications

References 7 publications

Sensors for Catheter Applications

Sensors for Catheter Applications

Avidin-biotin coupling as a general method for preparing enzyme-based fiber-optic sensors

Surface plasmon resonance based fiber optic glucose biosensor

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