Enzyme-assisted extraction of anticoagulant polysaccharide from Liparis tessellatus eggs

Ticar, Bernadeth F.; Rohmah, Zuliyati; Ambut, Carmelo V.; Choi, Yeung Joon; Mussatto, Solange I.; Choi, Byeong-Dae

doi:10.1016/j.ijbiomac.2015.01.002

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…Much research has shown that different cellulase concentrations will significantly affect the yield of polysaccharides [31,32]. As shown in Figure 1c the cellulase concentrationcurve indicated that the first increase of enzyme concentration lead to an obvious RAMP extraction yield increase till 2.0%, and then began to decrease slightly.…”

Section: Resultsmentioning

confidence: 96%

Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function Approach

Pu¹,

Xia

Hu³

et al. 2015

Molecules

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Rhizoma Atractylodes macrocephala polysaccharides (RAMP) have been reported to have a variety of important biological activities. In this study, an ultrasonic-assisted enzymatic extraction (UAEE) was employed to obtain the highest extraction yield and strongest antioxidant activity of RAMP and optimized by a multi-response optimization process. A three-level four-factor Box-Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (DF) to attain the optimal extraction parameters. The DPPH scavenging percentage was used to represent the antioxidant ability of RAMP. The maximum D value (0.328), along with the maximum yield (59.92%) and DPPH scavenging percentage (13.28%) were achieved at 90.54 min, 57.99˝C, 1.95% cellulase and 225.29 W. These values were further validated and found to be in good agreement with the predicted values. Compared to the other extraction methods, both the yield and scavenging percentage of RAMP obtained by UAEE was favorable and the method appeared to be time-saving and of high efficiency. These results demostrated that UAEE is an appropriate and effective extraction technique. Moreover, RSM with DF approach has been proved to be adequate for the design and optimization of the extraction parameters for RAMP. This work has a wide range of implications for the design and operation of polysaccharide extraction processes.

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Section: Resultsmentioning

confidence: 96%

Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function Approach

Pu¹,

Xia

Hu³

et al. 2015

Molecules

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“…Polysaccharides from Liparis tessellatus eggs (PLE) were isolated according to the methodology described by Ticar et al (2015). For purification, a concentration of 10 mg/ml of polysaccharide sample was dissolved in deionized water, filtered in a 0.2-µm membrane and applied to a DEAE-sepharose™ fast flow column (2.5 × 30 cm).…”

Section: Isolation and Purification Of Polysaccharides From L Tesselmentioning

confidence: 99%

“…The fish species, L. tessellatus is a very good candidate for polysaccharides extraction, since it has low lipid content (0-0.10%) (Lee et al, 2012) and high carbohydrate content of almost 19.50 ± 1.1 % (Ticar et al, 2015). Its eggs contain mucopolysaccharides which are known for its anticoagulant and anti-thrombotic properties (Arivuselvan et al, 2011;Ticar et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Ticar

Rohmah

Mussatto

et al. 2017

Carbohydrate Polymers

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Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The ESI-MS/MS confirmed the presence of uronic acids on F3, which could be a 0,2 A2 fragment plus loss of methyl group which is very common among nonmethylated, sulfated disaccharides.

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“…This result can be explained by the fact that pH of the enzyme solution influences the enzyme activity. The effect of pH on enzyme activity was similar to the study by Ticar et al [36]. Therefore, pH 3.0–5.0 was chosen for BBD experiment.…”

Section: Resultsmentioning

confidence: 73%

Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment

Guo

Zhao

et al. 2015

Molecules

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A continuous process based on the combination of ultrasounds and/or microwaves pretreatments followed by enzymatic hydrolysis and simultaneous extraction (EHSE) has been proposed to recover genipin from Eucommia ulmoides bark. At first, in the pretreatment step, the mixture of 1.0 g dried bark powder and 10 mL deionized water were irradiated by microwave under 500 W for 10 min. Then, in hydrolysis step, the optimal conditions were as follows: 0.5 mg/mL of cellulase concentration, 4.0 pH of enzyme solution, 24 h of incubation time and 40 °C of incubation temperature. After incubation, 10 mL ethanol was added to extract genipin for 30 min by ultrasound. After EHSE treatment, the yield of genipin could reach 1.71 μmol/g. Moreover, scanning electron micrographs illustrated that severe structural disruption of plant was obtained by EHSE. The results indicated that the EHSE method provided a good alternative for the preparation of genipin from Eucommia ulmoides bark as well as other herbs.

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Enzyme-assisted extraction of anticoagulant polysaccharide from Liparis tessellatus eggs

Cited by 9 publications

References 24 publications

Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function Approach

Multi-Optimization of Ultrasonic-Assisted Enzymatic Extraction of Atratylodes macrocephala Polysaccharides and Antioxidants Using Response Surface Methodology and Desirability Function Approach

Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment

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