Enzyme activity in skeletal muscle tissues in guinea pigs under functional load

Abstract

Abstract. The study is aimed at identifying the nature and intensity of age-related changes in the activity of AsAT and AlAT enzymes under a load of “Proserin” in the tissues of the triceps brachii, pectoralis major muscle, rectus abdominis, gluteus and calf muscles in guinea pigs grown under the conditions of the Scientific and Educational Center “Studencheskiy” of FSBEI HE “Chuvash State Agricultural Academy”. Methodology and methods: The activity of the enzymes AsAT and AlAT was determined by the universal method of S. Wrightman and S. Frenkel (1957) in the modification of V. V. Menshikov (1977) by the spectrophotometric method in the scientific laboratory of Chuvash State Agricultural Academy. Statistical reliability was determined by Student's criterion according to R. Kh. Tukshaitov (2001). Results and scope: analysis of changes in the activity of the enzymes aspartate and alanine aminotransferases indicates that during loading by introducing “Proserin” in the tissues of skeletal muscles, regular changes in all studied parameters with certain features depending on the age of the animal and the type of skeletal muscle are detected. The consistency of changes in the studied parameters in guinea pigs of different ages is manifested in the fact that, under experimental conditions, an increase in the activity of alanine and aspartate aminotransferase enzymes in the tissues of skeletal muscles is detected. The scientific novelty of the study lies in the fact that under conditions of functional load it was revealed that changes in the activity of these enzymes are associated with muscle growth and development in the postnatal period, including various alternating phases of improving the structural and functional organization of organs and the body as a whole.