Enzyme activities of human myocardium: Creatine kinase and hexose phosphate isomerase

Haralambie, G; Uy, J.

doi:10.1002/clc.4960030307

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…However, storage of freeze-dried material and subsequent exposure to room air pressure lowers the CK activities and such an exposure should thus be minimized. In the present study, the room exposure was less than 2 h. Assuming the water content of skeletal muscle and myocardium to be 77 and 79% respectively (Bergstrom, 1962;Documenta Geigy, 1971) the CK values obtained for these muscles were similar to those obtained from determinationson fresh muscle tissue (Haralambie, 1980).…”

Section: Methodological Considerationssupporting

confidence: 77%

“…An increased serum CK-MB activity has therefore been accepted to be a highly specific sign of myocardial injury (Sobeletal., 1979;Sylven & Bendz, 1978). However, reports on CK and CK-MB activities in human myocardium and skeletal muscle are inconsistent (Thorstensson et al, 1977;Grande et al, 1979;Haralambie, 1980). Furthermore, the functional role of the MB isozyme is as yet uncertain.…”

Section: Introductionmentioning

confidence: 99%

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Human myocardial and skeletal muscle enzyme activities: creatine kinase and its isozyme MB as related to citrate synthase and muscle fibre types

Sylvén

Jansson

Olin

1983

Clinical Physiology

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Activities of myocardial and skeletal muscle total creatine kinase (CK) and its isozyme MB were related to the oxidative capacity [measured as the citrate synthase (CS) activity] and to the contractile characteristics (estimated as the percentage of type I muscle fibres). Skeletal muscle biopsies were obtained both from physically trained and untrained men and myocardial biopsies from patients subjected to open-heart surgery performed because of mitral or aortic valve disease. Enzyme activities were determined on freeze-dried muscle specimens. The CK-MB activity was about twice as high in trained skeletal muscle as in untrained ones reaching the myocardial level. The total CK activity was about three times higher in skeletal muscle than in myocardium; the myocardium, however, had CS activity 3-4 times larger than that of skeletal muscle. A close correlation was demonstrated between activities of CK-MB on one hand and CS (r = 0.76) or percentage type I fibres (r = 0.83) on the other hand suggesting a connection between CK-MB activity and the oxidative capacity of the cell. This was in contrast to total CK where different regressions were obtained when comparing the myocardium and the skeletal muscle of trained or untrained men. In conclusion, CK-MB activity in trained skeletal muscle in athletes were similar to that in myocardium. CK-MB was related to the oxidative capacity and formation of cellular energy in skeletal and heart muscle.

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Section: Methodological Considerationssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Human myocardial and skeletal muscle enzyme activities: creatine kinase and its isozyme MB as related to citrate synthase and muscle fibre types

Sylvén

Jansson

Olin

1983

Clinical Physiology

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“…The absolute creatine kinase values for autopsy specimens from our study are generally higher äs compared to former studies using biopsies (54,58,(70)(71)(72)(73), a finding arguing indirectly against a marked autolytic change for this enzyme in heart and skeletal muscle. Higher values have been reported only by Haralambie & Uy (74) for human left papillary muscle. This is probably due to the well known differences between the various functional areas of the heart (73).…”

Section: Ultra-turrax) and Fractionated Extraction (Bücher)mentioning

confidence: 51%

Catalytic Enzyme Activity Concentration in Tissues of Man, Dog, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology, III. Communication

Lindena¹,

Sommerfeld²,

Höpfel³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary:The catalytic aetivity of up to fifteen enzymes was investigated in the liver, heart, skeletal muscle, kidney (medulla, cortex), brain, hing, duodenum, spieen and pancreas from man and animals. Human specimens were obtained from autopsies and immediately post-mortem from dogs, rabbits, guinea pigs, rats and mice. The differences between our results and previous reports of considerably lower activities for structural enzymes (e. g. creatine kinase) and for enzymes partly of mitochondrial origin (e. g. glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase), is attributed to our use of a detergent extraction technique. The superiority of the detergent technique with regard to enzyme yield is exemplified by a comparison of various methods of extraction in rat liver, heart and skeletal muscle. Use of standardized assays allows a qualitative inter-species comparison of results. The influence of autolysis on catalytic aetivity of human autopsies is considered of minor importance. Katalytische Enzymaktivitätskonzentration in Geweben von

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The Creatine Kinase System in Normal and Diseased Human Myocardium

Ingwall¹,

Kramer²,

Fifer³

et al. 1985

N Engl J Med

376

141

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We measured creatine kinase activity, isozyme composition, and total creatine content in biopsy samples of left ventricular myocardium from 34 adults in four groups: subjects with normal left ventricles, patients with left ventricular hypertrophy due to aortic stenosis, patients with coronary artery disease without left ventricular hypertrophy, and patients with coronary artery disease and left ventricular hypertrophy due to aortic stenosis. As compared with specimens of normal left ventricles, those from all patients with left ventricular hypertrophy had lower creatine kinase activity, higher MB creatine kinase isozyme content and activity, and lower creatine content. Specimens from the patients without left ventricular hypertrophy had normal creatine kinase activity, increased MB creatine kinase isozyme content and activity, and decreased total creatine content. The normal ventricles showed almost no MB isozyme content or activity. These data suggest that changes in the creatine kinase system occur in both pressure-overload hypertrophy and coronary artery disease. Patients with myocardial infarction who have mild or no preexisting fixed coronary artery disease or pressure-overload hypertrophy would not be expected to have elevation of serum MB creatine kinase.

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Enzyme activities of human myocardium: Creatine kinase and hexose phosphate isomerase

Cited by 3 publications

References 15 publications

Human myocardial and skeletal muscle enzyme activities: creatine kinase and its isozyme MB as related to citrate synthase and muscle fibre types

Human myocardial and skeletal muscle enzyme activities: creatine kinase and its isozyme MB as related to citrate synthase and muscle fibre types

Catalytic Enzyme Activity Concentration in Tissues of Man, Dog, Rabbit, Guinea Pig, Rat and Mouse. Approach to a Quantitative Diagnostic Enzymology, III. Communication

The Creatine Kinase System in Normal and Diseased Human Myocardium

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