Enzyme activities in the tibialis anterior muscle of young moderately active men and women: relationship with body composition, muscle cross‐sectional area and fibre type composition

Jaworowski, Åsa; Porter, M; Holmbäck, Anna Maria; Downham, David; Lexell, Jan

doi:10.1046/j.1365-201x.2002.t01-2-01004.x

Cited by 86 publications

(69 citation statements)

References 49 publications

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“…The proportion of time subjects were physically active at a low, moderate, and high intensity was also comparable between sexes, although %High was significantly higher in men: 18 min/day vs. 9 min/day in women (P Ͻ 0.01, 95% CI: 2.4 to 14.3). No significant differences between men and women were found for CS, SDH, COX, or HAD, which confirms the findings of previous studies (14,26,29). Furthermore, no interaction was observed between sex and PA DL for the capacity of the mitochondrial enzymes.…”

Section: Resultssupporting

confidence: 81%

Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity

Hoed

Hesselink²,

Kranenburg³

2008

Journal of Applied Physiology

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den Hoed M, Hesselink MK, van Kranenburg GP, Westerterp KR. Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity. J Appl Physiol 105: 561-568, 2008. First published May 29, 2008 doi:10.1152/japplphysiol.00091.2008.-Physical exercise training is a powerful tool to maintain or improve mitochondrial density and function (mitochondrial capacity). This study aims to determine whether mitochondrial capacity is also associated with habitual physical activity in daily life (PA DL). The capacity of classic markers for mitochondrial density, i.e., the capacity of citrate synthase (CS) and succinate dehydrogenase (SDH), as well the capacity of cytochrome c oxidase (COX) and ␤-hydroxyacyl-CoA dehydrogenase (HAD), was determined in homogenized muscle biopsy samples obtained from the vastus lateralis muscle of nonexercising healthy young (age 20 Ϯ 2 yr) subjects (31 women, 7 men). PA DL was measured during two periods of 14 days using a triaxial accelerometer for movement registration. CS, SDH, and COX were positively associated with PA DL [P Ͻ 0.05, R ϭ 0.36, 95% confidence interval (CI): 1.3 ⅐ 10 Ϫ4 to 2.2 ⅐ 10 Ϫ3 ; P Ͻ 0.05, R ϭ 0.39, 95% CI: 1.1 ⅐ 10 Ϫ5 to 9.9 ⅐ 10 Ϫ5 ; and P Ͻ 0.05, R ϭ 0.33, 95% CI: 7.5 ⅐ 10 Ϫ6 to 3.6 ⅐ 10 Ϫ4 , respectively], and HAD tended to correlate positively with PA DL (P ϭ 0.06, R ϭ 0.31, 95% CI: Ϫ2.2 ⅐ 10 Ϫ5 to 1.1 ⅐ 10 Ϫ3 ). The population was subsequently stratified based on the intensity of the activities performed. CS was only associated with PADL in subjects spending more time on high-intensity physical activity, whereas HAD was only associated with PADL in subjects spending less time on low intensity physical activity. We are the first to report that even within the range of normal daily life activities, mitochondrial capacity is positively associated with the level of habitual physical activity in daily life. Thus an active lifestyle may help to maintain or improve mitochondrial capacity. accelerometer; intensity; citrate synthase; succinate dehydrogenase; cyctochrome c oxidase; ␤-hydroxyacyl-CoA dehydrogenase IN WESTERNIZED SOCIETIES, the prevalence of Type 2 diabetes and obesity has increased significantly over the past few decades (18,25). A decreased physical activity has been associated with both Type 2 diabetes (13, 17) and obesity (15). Part of the pathophysiology related to these diseases has been attributed to a reduced mitochondrial capacity: the product of mitochondrial density and function (2,16,22).Mitochondria are vital organelles in the oxidative degradation of macronutrients to maintain cellular ATP levels. Mitochondrial aberrations, resulting in a reduced mitochondrial capacity, may therefore seriously impair normal energy and substrate metabolism. Mitochondrial aberrations have not only been reported for metabolic disorders like Type 2 diabetes and obesity but also in diseases like chronic heart failure and chronic obstructive pulmonary disease (11, 24).Proper mitochondrial function and maintenance of mitochondrial capac...

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Section: Resultssupporting

confidence: 81%

Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity

Hoed

Hesselink²,

Kranenburg³

2008

Journal of Applied Physiology

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“…According to the model, S1 is greater than or equal to S2 , and it is associated with diffusive transport parallel to the fibers. This is in agreement with findings from the literature, 19,25 where 1 has been associated with diffusive transport along the long axis of highly ordered fibers, with 2 and 3 being orthogonal to 1 . From eqns (6a) and (6b), the simulated fractional anisotropy can be calculated, which is given as…”

supporting

confidence: 92%

“…Some of this research includes studying differences in metabolism, 1 venous pressure, 2 fatigue, 3 structure 4,5 and architecture. 6 At present, most of the techniques used for studying muscle physiology and structure have relied on invasive procedures, such as muscle biopsies.…”

Section: Introductionmentioning

confidence: 99%

A diffusion tensor imaging analysis of gender differences in water diffusivity within human skeletal muscle

et al. 2005

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The diffusive properties of adjacent muscles at rest were evaluated in male (n = 12) and female (n = 12) subjects using diffusion tensor imaging (DTI). The principle, second and third eigenvalues, trace of the diffusion tensor [Tr(D)], and two anisotropic parameters, ellipsoid eccentricity (e) and fractional anisotropy (FA), of various muscles in the human calf were calculated from the diffusion tensor. Seven muscles were investigated in this study from images acquired of the left calf: the soleus, lateral gastrocnemius, medial gastrocnemius, posterior tibialis, anterior tibialis, extensor digitorum longus and peroneus longus. A mathematical model was also derived that relates the eigenvalues of the diffusion tensor to the muscle fiber volume fraction, which is defined as the volume of muscle fibers within a well-defined arbitrary muscle volume. Females on average had higher eigenvalues and Tr(D) compared with males, with the majority of muscles being statistically different between the sexes. In contrast, males on average had higher e and FA than females, with the large plantar flexors--soleus, lateral gastrocnemius, and medial gastrocnemius--producing statistically different results. The behavior of the mathematical model for variations in fiber volume fraction produced similar trends to those seen when the experimental data were fit to the model. The model predicts that a larger volume fraction of skeletal muscle in males is devoted to fibers than in females, but the true underlying source of the gender discrepancy remains unclear. Although the model does not fully account for other transport processes, it does provide some insight into the limiting factors that affect the diffusion of water in skeletal muscle measured by DTI.

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“…20 Although MHC isoform composition is a major determinant of muscle contractile properties, prior studies reveal only a weak correlation between human muscle MHC isoform and muscle enzymatic activity. 23,31 This poor correlation may be due in part to different synthesis rates for MHC compared to sarcoplasmic proteins in the aged population. 4 A dissociation between MHC phenotype and metabolic properties has been reported in paralyzed muscle following spinal cord injury.…”

Section: Discussionmentioning

confidence: 95%

Muscle molecular phenotype after stroke is associated with gait speed

et al. 2004

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The disability of patients after stroke is generally attributed to upper motor neuron defects, but secondary changes in paretic muscle may enhance the disability. We analyzed the molecular phenotype and metabolic profile of the paretic and nonparetic vastus lateralis (VL) and we measured the severity of gait deficit in 13 patients at least 6 months after ischemic stroke. The results showed a significant increase in the proportion of fast myosin heavy chain (MHC, 68 +/- 14%) in the paretic compared to the nonparetic VL (50 +/- 13%). The specific activity of citrate synthase and glyceraldehyde phosphodehydrogenase was not significantly different between the two sides. The proportion of fast MHC was inversely associated with severity of gait deficit indexed by self-selected walking speed in the paretic leg, but not the nonparetic leg. Our findings demonstrate significant and potentially modifiable secondary biologic changes in hemiparetic muscle phenotype that may contribute to the disability of stroke.

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Enzyme activities in the tibialis anterior muscle of young moderately active men and women: relationship with body composition, muscle cross‐sectional area and fibre type composition

Abstract: Enzyme activities in the tibialis anterior muscle of young moderately active men and women: relationship with body composition, muscle cross-sectional area and fibre type composition.

Cited by 86 publications

References 49 publications

Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity

Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity

A diffusion tensor imaging analysis of gender differences in water diffusivity within human skeletal muscle

Muscle molecular phenotype after stroke is associated with gait speed

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