Enzymatisch gesteuerte Freisetzung von Zoosporen bei Chlamydomonas reinhardii Dangeard in Synchronkultur

Schlösser, U. G.

doi:10.1007/bf00408711

Cited by 53 publications

(8 citation statements)

References 22 publications

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“…As reported previously, the culture medium of Chlamydomonas wild-type strains contains fragments of the insoluble glycoprotein framework of the Chlamydomonas cell wall that are released both during the cell enlargement period (Liebich and Voigt, 1995;Voigt et al, 1996) and during the liberation of zoospores by degradation of the mother cell wall (Schlösser, 1966;Mihara and Hase, 1975;Waffenschmidt et al, 1988). The culture medium of wall-deficient strains such as cw-15, however, contains constituents of the chaotrope-soluble wall layers and components normally localized in the periplasm (Loppes and Deltour, 1975;Voigt, 1986;Voigt et al, 1997).…”

Section: Analyses Of Macromolecules Accumulated In the Culture Mediummentioning

confidence: 80%

14-3-3 Proteins Are Constituents of the Insoluble Glycoprotein Framework of the Chlamydomonas Cell Wall

Voigt

Frank

2003

Plant Cell

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The cell wall of the unicellular green alga Chlamydomonas reinhardtii consists predominantly of Hyp-rich glycoproteins, which also occur in the extracellular matrix of multicellular green algae and higher plants. In addition to the Hyp-rich polypeptides, the insoluble glycoprotein framework of the Chlamydomonas cell wall contains minor amounts of 14-3-3 proteins, as revealed by immunochemical studies and mass spectroscopic analysis of tryptic peptides. Polypeptides immunologically related to the 14-3-3 proteins also were found in the culture medium of Chlamydomonas. The levels of two of these 14-3-3-related polypeptides were decreased in the culture medium of the wall-deficient mutant cw-15 . These findings indicate that 14-3-3 proteins are involved in the cross-linking of Hyp-rich glycoproteins in the Chlamydomonas cell wall.

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Section: Analyses Of Macromolecules Accumulated In the Culture Mediummentioning

confidence: 80%

14-3-3 Proteins Are Constituents of the Insoluble Glycoprotein Framework of the Chlamydomonas Cell Wall

Voigt

Frank

2003

Plant Cell

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“…For determination of flagellar lengths cells and sporangia were fixed in Lugol's solution according to Schlosser (1966). Flagellar lengths of 30 different cells from each sample were measured by light microscopy using a Zeiss inverted microscope (IM35) equipped with Nomarski interference optics and a 100x/1.25 oil immersion planachromate objective lens.…”

Section: Measurements Ofjlagellar Lengthmentioning

confidence: 99%

Flagellar Development During the Cell Cycle in Chlamydomonas reinhardtii

Madey

Melkonian

1990

Botanica Acta

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Flagellar development during the asexual synchronous cell cycle of Chlamydomonas reinhardtii (11.32 aM) was studied by light microscopy. Cell walls of sporangia of different developmental status were dissolved using gamete lysin (g-lysin) enabling direct observation of flagellar development. Flagellar growth in progeny cells exhibits a linear kinetic with a growth rate of 28nm/min at 30°C leading to a flagellar length of 7-7.5 pm in 4-4.5 h. After this time the flagellar growth rate drops to 2.8 n d m i n (as in interphase). Both flagella of a single cell and all flagella within a sporangium grow out at the same time and with the same rate. Cycloheximide (10 pg/ml) completely blocks flagellar development. If cycloheximide is removed flagellar growth resumes at the normal rate with no lag-phase.Flagellar development during the cell cycle in C. reinhardtii differs considerably from the wellstudied model system of flagellar regeneration following amputation in the same species.

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“…A delay in cell division was induced by blue light but not by illumination with red or far-red light. The equal intensity action spectrum revealed two peaks at 400 and 500 nm.Photoautotrophically growing cultures of unicellular green algae can be synchronized by alternating periods of light and darkness (18,20,23). In the case of Chlamydomonas reinhardtii, synchronization by light/dark cycling was also achieved in the presence of acetate (8,26), which is metabolized by this phytoflagellate.…”

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confidence: 99%

Blue Light Regulation of Cell Division in Chlamydomonas reinhardtii

Münzner

Voigt²

1992

Plant Physiol.

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A delay in cell division was observed when synchronized cultures of the unicellular green alga Chiamydomonas reinhardtii growing under heterotrophic conditions were exposed to white light during the second half of the growth period. This effect was also observed when photosynthesis was blocked by addition of the photosystem 11 inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Light pulses of 10 minutes were sufficient to induce a delay in cell division in the presence or absence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. A delay in cell division was induced by blue light but not by illumination with red or far-red light. The equal intensity action spectrum revealed two peaks at 400 and 500 nm.Photoautotrophically growing cultures of unicellular green algae can be synchronized by alternating periods of light and darkness (18,20,23). In the case of Chlamydomonas reinhardtii, synchronization by light/dark cycling was also achieved in the presence of acetate (8,26), which is metabolized by this phytoflagellate. C reinhardtii cultures synchronized in the presence of acetate continue to divide synchronously for one cell cycle period when transferred to heterotrophic growth conditions (26). This finding enabled us to investigate the differential effects of light on cell growth and cell division. Illumination at the beginning of the growth period caused an increased growth rate. As a consequence, the cells entered the division phase earlier than dark-grown cells (26). However, when the cultures were exposed to light after the cells had doubled their mass, a considerable delay in cell division was observed, which was accompanied by an extended growth period (26). The light-induced delay in cell division was also observed in the presence of DCMU, an inhibitor of PSII.These observations indicate that the transition from cell growth to cell division is regulated by a light/dark-responsive cell cycle switch in C. reinhardtii (26) and that photosynthesis is apparently not involved in this process. Therefore, we have investigated which wavelengths and intensities of light are effective in inducing a delay in cell division. The aim of these 'Supported by a grant (Vo 327/1) from the Deutsche Forschungsgemeinschaft.

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Enzymatisch gesteuerte Freisetzung von Zoosporen bei Chlamydomonas reinhardii Dangeard in Synchronkultur

Cited by 53 publications

References 22 publications

14-3-3 Proteins Are Constituents of the Insoluble Glycoprotein Framework of the Chlamydomonas Cell Wall

14-3-3 Proteins Are Constituents of the Insoluble Glycoprotein Framework of the Chlamydomonas Cell Wall

Flagellar Development During the Cell Cycle in Chlamydomonas reinhardtii

Blue Light Regulation of Cell Division in Chlamydomonas reinhardtii

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