Enzymatically controlled material design with casein—From defined films to localized deposition of particles

Strube, Oliver I.; Rüdiger, Arne A.; Bremser, Wolfgang

doi:10.1016/j.jbiotec.2014.10.019

Cited by 15 publications

(21 citation statements)

References 15 publications

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“…No single casein micelles are distinguishable as they have undergone full coalescence, which is in accordance with our previous results [20].…”

supporting

confidence: 93%

“…If this destabilization occurs in direct proximity to a support surface, immediate deposition follows due to hydrophobic interactions. Besides other results, it could be shown that the process yields continuous layers of casein if the enzyme is tethered by physical adsorption [20]. 6 In this work, we present a proof of concept study for the applicability of this process in adhesion technology.…”

Section: Conceptmentioning

confidence: 80%

“…Recently, we developed a biological autodeposition process that enables the controlled deposition of biological particles on surfaces [20]. The process utilizes enzymatic reactions to change solubility of certain biopolymers.…”

Section: Conceptmentioning

confidence: 99%

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Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

Strube

Rüdiger

Bremser

2015

International Journal of Adhesion and Adhesives

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“…No single casein micelles are distinguishable as they have undergone full coalescence, which is in accordance with our previous results [20].…”

supporting

confidence: 93%

Section: Conceptmentioning

confidence: 80%

See 1 more Smart Citation

Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

Strube

Rüdiger

Bremser

2015

International Journal of Adhesion and Adhesives

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“…This enables the possibility for a novel kind of material design. [56][57][58] The herein described studies are based on the system casein/chymosin. Usage of proteases like chymosin plays an important role in industrial processes, such as food biotechnology, cleaning processes, or the leather industry, because enzymatic hydrolysis of peptide bonds is very specific and easily controllable.…”

Section: Concept Of the Enzyme-mediated Autodepositionmentioning

confidence: 99%

“…56 Furthermore, this process is not limited to the system casein/chymosin, but is also transferable to other systems. For instance, it can be applied to synthesize and to specifically deposit eumelanin nanoparticles via an in situ reaction close to a given support surface.…”

Section: Concept Of the Enzyme-mediated Autodepositionmentioning

confidence: 99%

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

2016

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The enzyme-mediated autodeposition on the example of casein is reviewed, and deposition of casein structures is investigated in relation to applied immobilization methods of enzyme. First, casein is described in detail with respect to its structure in aqueous environments, followed by presentation of historical and current nonfood applications of casein. The presented process uses enzymes to trigger deposition of bio-based particles in close proximity to a support surface and allows for a high control over film formation and site-specificity. This is ensured by immobilization of enzyme on the support material. The herein described system is based on casein as protein and chymosin as enzyme. Different immobilization methods are investigated with respect to obtainable casein coatings, layers, and structures. Physical adsorption of enzyme enables the formation of casein coatings with controllable film thickness and is suitable for in situ buildup of adhesive layers due to diffusion of enzyme. The highest control over film formation is provided by covalent attachment of enzyme. Based on the attained results, the enzymemediated autodeposition gives new insights into biological material design. Graphical abstractEnzyme Casein micelle intact Bulk zone (no enzyme) Reaction zone Phe Met Support Support Casein micelle cleaved

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Enzyme‐Mediated In Situ Synthesis and Deposition of Nonaggregated Melanin Protoparticles

Strube

Büngeler

Bremser³

2016

Macro Materials & Eng

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The previously introduced process for enzyme‐mediated in situ synthesis and deposition of eumelanin is investigated with covalent immobilization of the tyrosinase. It results in a monolayer structure of non‐coalesced melanin particles, with a film thickness of 5–8 nm. The reaction is self‐terminating due to overlay of the enzymes with particles. The melanin particles are rodlike with lengths down to 6 nm. Isolated melanin structures of such small size have not been observed before and might be a kind of protoparticle in the supramolecular buildup of melanin oligomers. Utilization of melanin particles with such small size can enable nanotechnological applications in the areas of bioelectronics and biosensors.

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Enzymatically controlled material design with casein—From defined films to localized deposition of particles

Cited by 15 publications

References 15 publications

Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

Enzyme‐Mediated In Situ Synthesis and Deposition of Nonaggregated Melanin Protoparticles

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