Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

Strube, Oliver I.; Rüdiger, Arne A.; Bremser, Wolfgang

doi:10.1016/j.ijadhadh.2015.08.001

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…This enables the possibility for a novel kind of material design. [56][57][58] The herein described studies are based on the system casein/chymosin. Usage of proteases like chymosin plays an important role in industrial processes, such as food biotechnology, cleaning processes, or the leather industry, because enzymatic hydrolysis of peptide bonds is very specific and easily controllable.…”

Section: Concept Of the Enzyme-mediated Autodepositionmentioning

confidence: 99%

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The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

2016

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The enzyme-mediated autodeposition on the example of casein is reviewed, and deposition of casein structures is investigated in relation to applied immobilization methods of enzyme. First, casein is described in detail with respect to its structure in aqueous environments, followed by presentation of historical and current nonfood applications of casein. The presented process uses enzymes to trigger deposition of bio-based particles in close proximity to a support surface and allows for a high control over film formation and site-specificity. This is ensured by immobilization of enzyme on the support material. The herein described system is based on casein as protein and chymosin as enzyme. Different immobilization methods are investigated with respect to obtainable casein coatings, layers, and structures. Physical adsorption of enzyme enables the formation of casein coatings with controllable film thickness and is suitable for in situ buildup of adhesive layers due to diffusion of enzyme. The highest control over film formation is provided by covalent attachment of enzyme. Based on the attained results, the enzymemediated autodeposition gives new insights into biological material design. Graphical abstractEnzyme Casein micelle intact Bulk zone (no enzyme) Reaction zone Phe Met Support Support Casein micelle cleaved

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Section: Concept Of the Enzyme-mediated Autodepositionmentioning

confidence: 99%

“…Subsequently, protein layers form on both surfaces and generate an adhesive layer after contact, gluing them together. 57 Figure 4 shows the general setup for the buildup of adhesive layers via enzyme-mediated autodeposition.…”

Section: Casein Deposition Via Adsorbed Enzymementioning

confidence: 99%

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

2016

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“…There has been a growing interest in micellar casein and casein fractions (αS-, β-and κ-casein fractions) because of its valuable properties, such as emulsifying and foam-stabilising properties [20], bio-functional properties [21], non-food applications (e.g., coating agents and glues [6,22]. Therefore, isolation of casein and casein fractions have been intensively studied.…”

Section: Resultsmentioning

confidence: 99%

Kazein Bazlı Yapıştırıcıların Üretimi ve Fizikokimyasal Özellikleri

ATAMER

2022

Akademik Gıda

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In this study, different adhesives manufactured by using various casein powders (micellar casein, αS-, β-and ĸ-casein fractions, sodium caseinate and calcium caseinate) were investigated for their properties and potential application in the food industry. Casein-based adhesives using different sources of caseins were produced and the differences in their adhesive strength were determined. For the isolation of casein fractions, the methods of selective solubility, precipitation and a decanter centrifuge (for separation of precipitated casein and supernatant) were used. Achieved purities of αS-, β-and κ-casein fractions were higher than 25, 91 and 54%, respectively. Results showed that the type of casein raw material used in the production of adhesives had an influence on the adhesive properties and the highest adhesive strength was achieved with the enriched αS-casein fraction and micellar casein.

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“…The preparation of the protein layers on the surface of the transducers is among common applications of acoustic biosensors. For example, the preparation of casein layers is attractive for the study of their physicochemical properties and for applications in the pharmaceutical and food industries [ 58 ].…”

Section: Surface Acoustic Methodsmentioning

confidence: 99%

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Dizon

Tatarko

Hianik

2020

Sensors

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This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.

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Buildup of biobased adhesive layers by enzymatically controlled deposition on the example of casein

Cited by 15 publications

References 19 publications

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

The enzyme-mediated autodeposition of casein: effect of enzyme immobilization on deposition of protein structures

Kazein Bazlı Yapıştırıcıların Üretimi ve Fizikokimyasal Özellikleri

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

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